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AB278619

Anti-MKK4 (phospho S257) antibody [SEK1MKK4S257-C5]

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(1 Publication)

Rabbit Recombinant Monoclonal MEK4/MKK4 phospho S257 antibody. Suitable for Flow Cyt, WB and reacts with Mouse, Human, African green monkey samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MAP2K4 phospho S257.

View Alternative Names

JNKK1, MEK4, MKK4, PRKMK4, SEK1, SERK1, SKK1, MAP2K4, Dual specificity mitogen-activated protein kinase kinase 4, MAP kinase kinase 4, MAPKK 4, JNK-activating kinase 1, MAPK/ERK kinase 4, SAPK/ERK kinase 1, Stress-activated protein kinase kinase 1, c-Jun N-terminal kinase kinase 1, MEK 4, SAPK kinase 1, SAPKK-1, SAPKK1, JNKK

5 Images
Flow Cytometry - Anti-MKK4 (phospho S257) antibody [SEK1MKK4S257-C5] (AB278619)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-MKK4 (phospho S257) antibody [SEK1MKK4S257-C5] (AB278619)

Flow cytometric analysis of HEK-293T cells treated with lambda phosphatase (red) or treated with UV+TPA (green) using ab278619, at 0.1 ug/mL, or concentration-matched Rabbit (G9) mAb IgG Isotype Control for cells treated with phosphatase (black) or treated with UV+TPA (blue).

Flow Cytometry - Anti-MKK4 (phospho S257) antibody [SEK1MKK4S257-C5] (AB278619)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-MKK4 (phospho S257) antibody [SEK1MKK4S257-C5] (AB278619)

Flow cytometric analysis of 3T3 cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using ab278619 at 0.1 µg/mL.

Western blot - Anti-MKK4 (phospho S257) antibody [SEK1MKK4S257-C5] (AB278619)
  • WB

Supplier Data

Western blot - Anti-MKK4 (phospho S257) antibody [SEK1MKK4S257-C5] (AB278619)

All lanes:

Western blot - Anti-MKK4 (phospho S257) antibody [SEK1MKK4S257-C5] (ab278619) at 0.05 µg/mL

Lane 1:

THP1 cell extract treated with sorbitol

Lane 2:

THP1 cell extract untreated

false

Western blot - Anti-MKK4 (phospho S257) antibody [SEK1MKK4S257-C5] (AB278619)
  • WB

Supplier Data

Western blot - Anti-MKK4 (phospho S257) antibody [SEK1MKK4S257-C5] (AB278619)

All lanes:

Western blot - Anti-MKK4 (phospho S257) antibody [SEK1MKK4S257-C5] (ab278619) at 0.05 µg/mL

Lane 1:

COS7 cell extract untreated

Lane 2:

COS7 cell extract treated with UV plus TPA

false

Western blot - Anti-MKK4 (phospho S257) antibody [SEK1MKK4S257-C5] (AB278619)
  • WB

Supplier Data

Western blot - Anti-MKK4 (phospho S257) antibody [SEK1MKK4S257-C5] (AB278619)

All lanes:

Western blot - Anti-MKK4 (phospho S257) antibody [SEK1MKK4S257-C5] (ab278619) at 0.05 µg/mL

Lane 1:

THP1 cell extract treated with sorbitol

Lane 2:

THP1 cell extract untreated

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SEK1MKK4S257-C5

Isotype

IgG

Light chain type

kappa

Carrier free

No

Reacts with

Mouse, Human, African green monkey

Applications

Flow Cyt, WB

applications

Immunogen

Synthetic Peptide within Human MAP2K4 phospho S257. The exact immunogen used to generate this antibody is proprietary information.

P45985

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.001-1 µg/mL", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.05 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.001-1 µg/mL", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "African green monkey": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.05 µg/mL", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 seems to be the prerequisite for JNK activation at least in response to pro-inflammatory cytokines, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs. MAP2K4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Whereas MAP2K7/MKK7 exclusively activates JNKs, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAPK12, MAPK13 and MAPK14.
See full target information MAP2K4 phospho S257
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biochemistry and biophysics reports 31:101329 PubMed36032400

2022

Development of a novel tocopheryl ester for suppression of lipid accumulation without cytotoxicity by optimization of dicarboxylic ester moiety.

Applications

Unspecified application

Species

Unspecified reactive species

Misaki Yamasaki,Yuika Seto,Mizune Ozono,Michiyasu Nakao,Akira Shigenaga,Akira Otaka,Shigeki Sano,Kentaro Kogure
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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