Rabbit Polyclonal MKK7 phospho S271 + T275 antibody. Suitable for WB and reacts with Recombinant full length protein samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human MAP2K7 phospho S271 + T275.
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Publications
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- Cell systems 13:885-894.e42022Systematic analysis of the MAPK signaling network reveals MAP3K-driven control of cell fate.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmy F Peterson,Kayla Ingram,E J Huang,Jeeun Parksong,Connor McKenney,Gabriel S Bever,Sergi RegotPubMed 36356576
- Oncology letters 22:8412021miR-152-mediated MKK7 downregulation is attenuated by MYCNOS in ovarian adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuifang Zhang,Dan Zheng,Xiaoqing Chen,Li Li,Jingrong YuPubMed 34733360
- Cell death and differentiation 25:1581-15972018Inhibition of the JNK/MAPK signaling pathway by myogenesis-associated miRNAs is required for skeletal muscle development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShu-Juan Xie et. al.PubMed 29449644
- PLoS pathogens 13:e10065342017Bacterial effector NleL promotes enterohemorrhagic E. coli-induced attaching and effacing lesions by ubiquitylating and inactivating JNK.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiangpeng Sheng et. al.PubMed 28753655
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human MAP2K7 phospho S271 + T275. The exact immunogen used to generate this antibody is proprietary information. Database link O14733
Reactivity data
Select an application| WB | |
|---|---|
| Mouse | Predicted |
| Recombinant full length protein | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
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Target data
Function
Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K4/MKK4, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4/MKK4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The monophosphorylation of JNKs on the Thr residue is sufficient to increase JNK activity indicating that MAP2K7/MKK7 is important to trigger JNK activity, while the additional phosphorylation of the Tyr residue by MAP2K4/MKK4 ensures optimal JNK activation. Has a specific role in JNK signal transduction pathway activated by pro-inflammatory cytokines. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Part of a non-canonical MAPK signaling pathway, composed of the upstream MAP3K12 kinase and downstream MAP kinases MAPK1/ERK2 and MAPK3/ERK1, that enhances the AP-1-mediated transcription of APP in response to APOE (PubMed:28111074).
Alternative names
JNKK2, MEK7, MKK7, PRKMK7, SKK4, MAP2K7, Dual specificity mitogen-activated protein kinase kinase 7, MAP kinase kinase 7, MAPKK 7, JNK-activating kinase 2, MAPK/ERK kinase 7, Stress-activated protein kinase kinase 4, c-Jun N-terminal kinase kinase 2, MEK 7, SAPK kinase 4, SAPKK-4, SAPKK4, JNK kinase 2, JNKK 2
Recommended products
Rabbit Polyclonal MKK7 phospho S271 + T275 antibody. Suitable for WB and reacts with Recombinant full length protein samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human MAP2K7 phospho S271 + T275.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human MAP2K7 phospho S271 + T275. The exact immunogen used to generate this antibody is proprietary information. Database link O14733
- Purification technique
- Affinity purification Immunogen
- Specificity
This antibody does not react with other MEK isoforms.
- Concentration
- Purification notes
Purified from rabbit serum by sequential epitope specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated MEK 7. The final product is generated by affinity chromatography using an MEK 7 derived peptide that is phosphorylated at serine 271 and threonine 275.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MKK7 also known as MAP2K7 or Mitogen-activated protein kinase kinase 7 is a dual-specificity kinase involved in the phosphorylation of target proteins. It functions as a critical member of the MAP2K family with a molecular mass of approximately 44 kDa. MKK7 is widely expressed in various tissues including the brain and immune cells which indicates its diverse functional roles across different biological systems.
- Biological function summary
MKK7 plays an essential role in the activation of the JNK (c-Jun N-terminal kinases) signaling pathway by phosphorylating and activating JNK. It operates as part of a signaling complex that responds to stress stimuli. This kinase is involved in numerous cellular processes such as apoptosis differentiation and inflammatory responses. The regulation of these processes by MKK7 impacts many aspects of cellular homeostasis and response to environmental changes.
- Pathways
MKK7 is integrally involved in the stress-activated MAPK pathway and the cellular response to inflammatory cytokines. It closely collaborates with related proteins such as JNK1 JNK2 and JNK3 to mediate its downstream effects. Activation of this pathway leads to the modulation of gene expression that governs cell survival proliferation and other critical cellular functions.
- Associated diseases and disorders
Dysregulation of MKK7 activity connects the protein to disorders like cancer and neurodegenerative diseases. Altered MKK7-JNK signaling contributes to the pathogenesis of certain cancers by affecting cell proliferation and survival mechanisms. In neurodegenerative diseases MKK7-mediated pathways are associated with neuronal stress responses and cell death highlighting its interaction with proteins like Amyloid-beta in Alzheimer's disease. Understanding MKK7's role in these conditions offers potential for therapeutic interventions.
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Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-MKK7 (phospho S271 + T275) antibody (ab4762)
Peptide Competition: Extracts prepared from background extracts with tagged fusion protein expressing MEK 7 added were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 0.50 μg/mL ab4762 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the
All lanes: Western blot - Anti-MKK7 (phospho S271 + T275) antibody (ab4762)
Predicted band size: 47 kDa
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