AB113264

Anti-Mkl1/MRTFA antibody

Name: Anti-Mkl1/MRTFA antibody (ab113264) | Abcam
Brand: Abcam Limited
SKU: AB113264

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(7 Publications)

Rabbit Polyclonal Mkl1/MRTFA antibody. Suitable for IHC-P and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human MRTFA aa 700-750.

View Alternative Names

KIAA1438, MAL, MKL1, MRTFA, Myocardin-related transcription factor A, MRTF-A, MKL/myocardin-like protein 1, Megakaryoblastic leukemia 1 protein, Megakaryocytic acute leukemia protein

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mkl1/MRTFA antibody (AB113264)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mkl1/MRTFA antibody (AB113264)

Formalin-fixed, paraffin-embedded section of Human breast carcinoma.
Antibody : ab113264 used at a dilution of 1/250.
Detection : DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human MRTFA aa 700-750. The exact immunogen used to generate this antibody is proprietary information.

Q969V6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: 99% Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mkl1 also known as MRTFA is a transcriptional coactivator that plays a role in regulating gene expression. It has a molecular weight of approximately 142 kDa. Mkl1 is widely expressed in various tissues including the heart lung and skeletal muscle. As a coactivator it interacts with serum response factor (SRF) to facilitate the transcription of target genes involved in cytoskeletal dynamics and cell differentiation processes.

Biological function summary

Mkl1 is important in the regulation of actin cytoskeleton remodeling. It does not act alone; it forms part of a dynamic complex with actin monomers. These interactions are essential for the modulation of gene transcription. Mkl1 binds to gene promoters enabling transcriptional activation in response to cellular signals that involve changes in actin polymerization.

Pathways

Mkl1 integrates into the RhoA signaling pathway and the Hippo pathway important for cytoskeleton organization and cell growth control respectively. Within these pathways Mkl1 regulates the activity of SRF coordinating cellular responses to mechanical stress and environmental stimuli. Mkl1 also interacts with other proteins such as myocardin and its family members which are relevant in smooth muscle cell differentiation.

Mutated or dysregulated Mkl1 has been connected to certain leukemias particularly acute megakaryoblastic leukemia. It also relates to cardiac hypertrophy a condition associated with abnormal heart muscle growth. Mkl1 dysregulation leads to altered SRF pathway activity impacting the expression of genes involved in cell growth and differentiation which is critical in these disease processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription coactivator that associates with the serum response factor (SRF) transcription factor to control expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration (PubMed : 26224645). The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics. MRTFA binds G-actin via its RPEL repeats, regulating activity of the MRTFA-SRF complex. Activity is also regulated by filamentous actin (F-actin) in the nucleus.

See full target information MRTFA

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

CNS neuroscience & therapeutics 31:e70578 PubMed40904192

2025

Endothelin-Converting Enzyme-Like 1 Regulated by LIF Contributes to Chronic Constriction Injury-Induced Neuropathic Pain in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Gao,Yuchen Pan,Yong Huang,Chen Gu,Xiaowei Song,Cunjin Wang

Molecular biology of the cell 35:ar133 PubMed39196658

2024

mDia2 is an important mediator of MRTF-A-dependent regulation of breast cancer cell migration.

Applications

Unspecified application

Species

Unspecified reactive species

Ian Eder,Virginia Yu,Jacob Antonello,Fangyuan Chen,David Gau,Pooja Chawla,Marion Joy,Peter C Lucas,David Boone,Adrian V Lee,Partha Roy

International journal of experimental pathology 104:64-75 PubMed36694990

2023

Synergistic activation of genes promoting invasiveness by dual deprivation in oxygen and nutrients.

Applications

Unspecified application

Species

Unspecified reactive species

Charly Jehanno,Yann Le Page,Gilles Flouriot,Pascale Le Goff,Denis Michel

Scientific reports 11:16096 PubMed34373467

2021

Benzalkonium chloride-induced myofibroblastic transdifferentiation of Tenon's capsule fibroblasts is inhibited by coculture with corneal epithelial cells or by interleukin-10.

Applications

Unspecified application

Species

Unspecified reactive species

Chiemi Yamashiro,Kazuhiro Tokuda,Yuka Kobayashi,Fumiaki Higashijima,Takuya Yoshimoto,Manami Ota,Tadahiko Ogata,Atsushige Ashimori,Masaaki Kobayashi,Makoto Hatano,Sho-Hei Uchi,Makiko Wakuta,Shinichiro Teranishi,Kazuhiro Kimura

Scientific reports 11:515 PubMed33436872

2021

Generation of proliferating human adult hepatocytes using optimized 3D culture conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Sophie Rose,Frédéric Ezan,Marie Cuvellier,Arnaud Bruyère,Vincent Legagneux,Sophie Langouët,Georges Baffet

Communications biology 3:637 PubMed33127987

2020

Tissue-scale tensional homeostasis in skin regulates structure and physiological function.

Applications

Unspecified application

Species

Unspecified reactive species

Shun Kimura,Ayako Tsuchiya,Miho Ogawa,Miki Ono,Nao Suda,Kaori Sekimoto,Makoto Takeo,Takashi Tsuji

Molecular and cellular endocrinology 390:34-44 PubMed24721635

2014

Activation of the MKL1/actin signaling pathway induces hormonal escape in estrogen-responsive breast cancer cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Gwenneg Kerdivel,Antoine Boudot,Denis Habauzit,Frederic Percevault,Florence Demay,Farzad Pakdel,Gilles Flouriot

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Mkl1/MRTFA antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mkl1/MRTFA antibody (AB113264)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise