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AB219981

Anti-Mkl1/MRTFA antibody [CL4281]

3

(1 Review)

|

(1 Publication)

Mouse Monoclonal Mkl1/MRTFA antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MRTFA aa 550-700.

View Alternative Names

KIAA1438, MAL, MKL1, MRTFA, Myocardin-related transcription factor A, MRTF-A, MKL/myocardin-like protein 1, Megakaryoblastic leukemia 1 protein, Megakaryocytic acute leukemia protein

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mkl1/MRTFA antibody [CL4281] (AB219981)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mkl1/MRTFA antibody [CL4281] (AB219981)

Immunohistochemical analysis of paraffin embedded Human testis tissue labeling Mkl1/MRTFA with ab219981 at 1/50 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mkl1/MRTFA antibody [CL4281] (AB219981)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mkl1/MRTFA antibody [CL4281] (AB219981)

Immunohistochemical analysis of paraffin embedded Human lymph node tissue labeling Mkl1/MRTFA with ab219981 at 1/50 dilution.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL4281

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MRTFA aa 550-700. The exact immunogen used to generate this antibody is proprietary information.

Q969V6

Epitope

Binds to an epitope located within the peptide sequence PTLITDSTGTHLVLT as determined by overlapping synthetic peptides.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mkl1 also known as MRTFA is a transcriptional coactivator that plays a role in regulating gene expression. It has a molecular weight of approximately 142 kDa. Mkl1 is widely expressed in various tissues including the heart lung and skeletal muscle. As a coactivator it interacts with serum response factor (SRF) to facilitate the transcription of target genes involved in cytoskeletal dynamics and cell differentiation processes.
Biological function summary

Mkl1 is important in the regulation of actin cytoskeleton remodeling. It does not act alone; it forms part of a dynamic complex with actin monomers. These interactions are essential for the modulation of gene transcription. Mkl1 binds to gene promoters enabling transcriptional activation in response to cellular signals that involve changes in actin polymerization.

Pathways

Mkl1 integrates into the RhoA signaling pathway and the Hippo pathway important for cytoskeleton organization and cell growth control respectively. Within these pathways Mkl1 regulates the activity of SRF coordinating cellular responses to mechanical stress and environmental stimuli. Mkl1 also interacts with other proteins such as myocardin and its family members which are relevant in smooth muscle cell differentiation.

Mutated or dysregulated Mkl1 has been connected to certain leukemias particularly acute megakaryoblastic leukemia. It also relates to cardiac hypertrophy a condition associated with abnormal heart muscle growth. Mkl1 dysregulation leads to altered SRF pathway activity impacting the expression of genes involved in cell growth and differentiation which is critical in these disease processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription coactivator that associates with the serum response factor (SRF) transcription factor to control expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration (PubMed : 26224645). The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics. MRTFA binds G-actin via its RPEL repeats, regulating activity of the MRTFA-SRF complex. Activity is also regulated by filamentous actin (F-actin) in the nucleus.
See full target information MRTFA
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biomaterials science 11:2886-2897 PubMed36880435

2023

Hydrogel mechanics regulate fibroblast DNA methylation and chromatin condensation.

Applications

Unspecified application

Species

Unspecified reactive species

Jenna L Sumey,Peyton C Johnston,Abigail M Harrell,Steven R Caliari
View all publications
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Product promise

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