JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB191496

Anti-Mkl2 antibody

Be the first to review this product! Submit a review

|

(4 Publications)

Rabbit Polyclonal Mkl2 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human MRTFB.

View Alternative Names

KIAA1243, MKL2, MRTFB, Myocardin-related transcription factor B, MRTF-B, MKL/myocardin-like protein 2, Megakaryoblastic leukemia 2

1 Images
Western blot - Anti-Mkl2 antibody (AB191496)
  • WB

Supplier Data

Western blot - Anti-Mkl2 antibody (AB191496)

All lanes:

Western blot - Anti-Mkl2 antibody (ab191496)

Lane 1:

HEK293T whole cell lysate

Lane 2:

NIH-3T3 whole cell lysate

Lane 3:

H9C2 whole cell lysate

Predicted band size: 118 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human MRTFB. The exact immunogen used to generate this antibody is proprietary information.

Q9ULH7

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1 - 1/5", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1 - 1/5", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1 - 1/5", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purity >95%.
Storage buffer
Preservative: 0.1% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Mkl2 protein also known as Myocardin-like 2 or MRTFB is a transcriptional coactivator that primarily interacts with serum response factor (SRF) to regulate gene expression. It has a molecular weight of approximately 118 kDa. Mkl2 is ubiquitously expressed but shows high levels of expression in heart and skeletal muscles. It predominantly resides in the cytoplasm but translocates to the nucleus upon activation.
Biological function summary

Mkl2 influences cytoskeletal dynamics and cellular motility by modulating actin cytoskeleton organization. It forms a complex with SRF to control the transcription of genes involved in these processes. This complex is essential for responding to environmental cues that promote cell differentiation and proliferation.

Pathways

Mkl2 is integral to the RhoA-MRTFA-SRF signaling pathway which links changes in the actin cytoskeleton to transcriptional responses. Mkl2 works in conjunction with proteins like MKL1 to mediate these pathway interactions. Together they coordinate responses to external stimuli that affect cellular architecture and movement.

Mkl2 has implications in cardiac hypertrophy and cancer progression. Aberrant expression or regulation of Mkl2 can lead to pathological cardiac growth impacting heart function. It also associates with oncogenes in the context of cancer promoting tumor cell invasion and metastasis. Understanding its interactions with proteins like MRTFA in these conditions provides potential therapeutic targets for treatment.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Acts as a transcriptional coactivator of serum response factor (SRF). Required for skeletal myogenic differentiation.
See full target information MRTFB
style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:3329 PubMed38337011

2024

Cellular senescence is associated with osteonecrosis of the femoral head while mesenchymal stem cell conditioned medium inhibits bone collapse.

Applications

Unspecified application

Species

Unspecified reactive species

Masanori Okamoto,Hiroaki Nakashima,Kiyoshi Sakai,Yasuhiko Takegami,Yusuke Osawa,Junna Watanabe,Sadayuki Ito,Hideharu Hibi,Shiro Imagama

Life science alliance 4: PubMed34389686

2021

Dystrophin involvement in peripheral circadian SRF signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Corinne A Betts,Aarti Jagannath,Tirsa LE van Westering,Melissa Bowerman,Subhashis Banerjee,Jinhong Meng,Maria Sofia Falzarano,Lara Cravo,Graham McClorey,Katharina E Meijboom,Amarjit Bhomra,Wooi Fang Lim,Carlo Rinaldi,John R Counsell,Katarzyna Chwalenia,Elizabeth O'Donovan,Amer F Saleh,Michael J Gait,Jennifer E Morgan,Alessandra Ferlini,Russell G Foster,Matthew Ja Wood

Iranian journal of basic medical sciences 22:745-751 PubMed32373295

2020

The effect of titanium dioxide nanoparticles on mice midbrain substantia nigra.

Applications

Unspecified application

Species

Unspecified reactive species

Zahra Heidari,Abbas Mohammadipour,Parisa Haeri,Alireza Ebrahimzadeh-Bideskan

Molecular medicine reports : PubMed32626937

2020

Downregulation of miR‑142‑5p inhibits human aortic smooth muscle cell proliferation and migration by targeting MKL2.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wu,Yuqiang Shang,Shiling Dai,Chunjun Yu,Jie Wang
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB242312

Anti-Mkl2 antibody [CL1546]

Immunocytochemistry/ Immunofluorescence - Anti-Mkl2 antibody [CL1546] (AB242312)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150155

Donkey Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed (AB150155)

  • 5
  • 2
Primary Antibodies

AB108986

Anti-PGP9.5 antibody [EPR4118] - Neuronal Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-PGP9.5 antibody [EPR4118] - Neuronal Marker (AB108986)

  • 5
  • 12
Primary Antibodies

AB221547

Anti-ZO1 tight junction protein antibody [EPR19945-296]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (AB221547)

  • 5
  • 6
Primary Antibodies

AB22048

Anti-TLR4 antibody [76B357.1]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR4 antibody [76B357.1] (AB22048)

  • 4
  • 29

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com