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AB242312

Anti-Mkl2 antibody [CL1546]

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(1 Publication)

Mouse Monoclonal Mkl2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MRTFB aa 100-300.

View Alternative Names

KIAA1243, MKL2, MRTFB, Myocardin-related transcription factor B, MRTF-B, MKL/myocardin-like protein 2, Megakaryoblastic leukemia 2

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Mkl2 antibody [CL1546] (AB242312)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Mkl2 antibody [CL1546] (AB242312)

Immunocytochemistry/Immunofluorescence analysis of A549 (Human lung carcinoma cell line) cells labeling Mkl2 using ab242312 at 4 μg/ml (green). Microtubules in red.

Western blot - Anti-Mkl2 antibody [CL1546] (AB242312)
  • WB

Supplier Data

Western blot - Anti-Mkl2 antibody [CL1546] (AB242312)

All lanes:

Western blot - Anti-Mkl2 antibody [CL1546] (ab242312) at 1 µg/mL

All lanes:

RT4 (Human urinary bladder cancer cell line) cell lysate

Predicted band size: 118 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-Mkl2 antibody [CL1546] (AB242312)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Mkl2 antibody [CL1546] (AB242312)

Immunocytochemistry/Immunofluorescence analysis of A549 (Human lung carcinoma cell line) cells labeling Mkl2 using ab242312 at 10 μg/ml (green). Microtubules in red.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL1546

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human MRTFB aa 100-300. The exact immunogen used to generate this antibody is proprietary information.

Q9ULH7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "<p>Fixation: PFA/Triton X-100</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Purified from TCS.
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Mkl2 protein also known as Myocardin-like 2 or MRTFB is a transcriptional coactivator that primarily interacts with serum response factor (SRF) to regulate gene expression. It has a molecular weight of approximately 118 kDa. Mkl2 is ubiquitously expressed but shows high levels of expression in heart and skeletal muscles. It predominantly resides in the cytoplasm but translocates to the nucleus upon activation.
Biological function summary

Mkl2 influences cytoskeletal dynamics and cellular motility by modulating actin cytoskeleton organization. It forms a complex with SRF to control the transcription of genes involved in these processes. This complex is essential for responding to environmental cues that promote cell differentiation and proliferation.

Pathways

Mkl2 is integral to the RhoA-MRTFA-SRF signaling pathway which links changes in the actin cytoskeleton to transcriptional responses. Mkl2 works in conjunction with proteins like MKL1 to mediate these pathway interactions. Together they coordinate responses to external stimuli that affect cellular architecture and movement.

Mkl2 has implications in cardiac hypertrophy and cancer progression. Aberrant expression or regulation of Mkl2 can lead to pathological cardiac growth impacting heart function. It also associates with oncogenes in the context of cancer promoting tumor cell invasion and metastasis. Understanding its interactions with proteins like MRTFA in these conditions provides potential therapeutic targets for treatment.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a transcriptional coactivator of serum response factor (SRF). Required for skeletal myogenic differentiation.
See full target information MRTFB
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 81:1337-1354.e8 PubMed33545068

2021

Systematically defining selective autophagy receptor-specific cargo using autophagosome content profiling.

Applications

Unspecified application

Species

Unspecified reactive species

Susanne Zellner,Martina Schifferer,Christian Behrends
View all publications
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Product promise

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