AB178403

Anti-Mkln1 antibody [EPR13519]

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal MKLN1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

Muskelin, MKLN1

2 Images

Supplier Data

Western blot - Anti-Mkln1 antibody [EPR13519] (AB178403)

All lanes:

Western blot - Anti-Mkln1 antibody [EPR13519] (ab178403) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

A431 cell lysate at 10 µg

Lane 3:

Y79 (Human retinoblastoma cell line) cell lysate at 10 µg

Lane 4:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 85 kDa

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Supplier Data

Immunoprecipitation - Anti-Mkln1 antibody [EPR13519] (AB178403)

Western blot analysis on immunoprecipitation pellet from 293T cell lysate labeling Mkln1 with ab178403 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-Mkln1 antibody [EPR13519] (ab178403)

Predicted band size: 85 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13519

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mkl1 also known as megakaryoblastic leukemia (translocation) 1 is a protein encoded by the MKL1 gene. The mass of Mkl1 approximately measures around 130 kDa. This protein is expressed in multiple tissues but it exhibits higher expression in the heart and skeletal muscle. Mkl1 localizes mainly in the nucleus where it functions as a coactivator of serum response factor (SRF) playing an important role in actin dynamics and transcriptional regulation.

Biological function summary

Mkl1 coordinates transcriptional programs required for cell differentiation and cytoskeletal organization. As a component of the MRTF-SRF complex Mkl1 regulates genes involved in actin cytoskeleton remodeling. This function impacts cell migration adhesion and morphology influenced by external signals. Additionally it plays a role in the regulation of smooth muscle and cardiac-specific gene expression which highlights its importance in muscle physiology.

Pathways

Mkl1 serves an important function in the RhoA-actin signaling pathway. This pathway controls the organization of the actin cytoskeleton and is connected with several cellular functions including cell shape and motility. Mkl1 interacts closely with SRF as well as other proteins like RhoA GTPase which are vital for transmitting signals that promote actin polymerization and cell movement. This pathway integration contributes to understanding how extracellular cues translate into cytoskeletal rearrangements.

Mkl1 has significant connections to megakaryoblastic leukemia and cardiac hypertrophy. In leukemia a chromosomal translocation involving Mkl1 and another protein MOZ leads to the production of an oncogenic fusion protein that causes dysregulation of normal gene expression contributing to malignancy. Additionally upregulated Mkl1 expression links to cardiac hypertrophy where it facilitates pathological gene expression in heart muscle cells. The role of Mkl1 in these disorders highlights its significance in disease progression and susceptibility.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1 (PubMed : 29911972). Required for internalization of the GABA receptor GABRA1 from the cell membrane via endosomes and subsequent GABRA1 degradation (By similarity). Acts as a mediator of cell spreading and cytoskeletal responses to the extracellular matrix component THBS1 (PubMed : 18710924).

See full target information MKLN1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nutrients 14: PubMed35683984

2022

Disruption of Glutamate Homeostasis in the Brain of Rat Offspring Induced by Prenatal and Early Postnatal Exposure to Maternal High-Sugar Diet.

Applications

Unspecified application

Species

Unspecified reactive species

Jozef Mizera,Bartosz Pomierny,Anna Sadakierska-Chudy,Beata Bystrowska,Lucyna Pomierny-Chamiolo

Neuron 99:1155-1169.e9 PubMed30174115

2018

Muskelin Coordinates PrP Lysosome versus Exosome Targeting and Impacts Prion Disease Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Frank F Heisler,Yvonne Pechmann,Ines Wieser,Hermann C Altmeppen,Leonhard Veenendaal,Mary Muhia,Michaela Schweizer,Markus Glatzel,Susanne Krasemann,Matthias Kneussel

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Mkln1 antibody [EPR13519]

Western blot - Anti-Mkln1 antibody [EPR13519] (AB178403)

Immunoprecipitation - Anti-Mkln1 antibody [EPR13519] (AB178403)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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