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AB168964

Anti-MKLP1 antibody

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(2 Publications)

Mouse Polyclonal MKLP1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human KIF23.

View Alternative Names

KNSL5, MKLP1, KIF23, Kinesin-like protein KIF23, Kinesin-like protein 5, Mitotic kinesin-like protein 1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-MKLP1 antibody (AB168964)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MKLP1 antibody (AB168964)

Immunofluorescence of paraformaldehyde-fixed HeLa cells labeling MKLP1 using ab168964 at 10 µg/ml.

Western blot - Anti-MKLP1 antibody (AB168964)
  • WB

Unknown

Western blot - Anti-MKLP1 antibody (AB168964)

All lanes:

Western blot - Anti-MKLP1 antibody (ab168964) at 1 µg/mL

Lane 1:

MKLP1 transfected 293T cell lysate at 15 µL

Lane 2:

Non-transfected 293T cell lysate at 15 µL

Predicted band size: 110 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human KIF23.

Q02241

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Alternative Products

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  • 0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MKLP1 also known as mitotic kinesin-like protein 1 is a motor protein with a molecular weight of around 95 kDa. This protein plays an important role in cell division by facilitating the movement of chromosomes and the formation of the spindle apparatus during mitosis. MKLP1 is widely expressed in proliferating cells and tissues especially in those undergoing rapid cell cycles such as embryonic tissues and tumors.
Biological function summary

MKLP1 functions as a component of the centralspindlin complex where it interacts with other proteins to regulate cytoskeletal dynamics. It is critical in the formation and resolution of the central spindle during mitosis ensuring proper separation of cells. By coordinating microtubule bundling and motor activities MKLP1 assists in the maintenance of cell polarity and cell shape changes necessary for mitosis completion.

Pathways

MKLP1 is integral to the mitotic and cell cycle pathways. In particular it is important in the mechanics of the spindle assembly checkpoint which ensures chromosomes are correctly segregated before cell division proceeds. MKLP1 interacts with proteins like Aurora B kinase and ECT2 which regulate the dynamics of cytoskeletal reorganization during these critical phases.

MKLP1 has associations with cancers and neurodevelopmental disorders. In tumors overexpression or mutation of MKLP1 often leads to aneuploidy contributing to tumor progression and metastasis. In the context of neurodevelopmental disorders disruptions in MKLP1 function can relate to conditions such as microcephaly due to its role in cell proliferation. Altered interactions between MKLP1 and proteins like BRCA2 in cancer highlight its relevance in both cell division and stability of the genome.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Essential for cytokinesis in Rho-mediated signaling. Required for the localization of ECT2 to the central spindle. Plus-end-directed motor enzyme that moves antiparallel microtubules in vitro.
See full target information KIF23
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 134: PubMed34152390

2021

Rab11FIP1 maintains Rab35 at the intercellular bridge to promote actin removal and abscission.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas V G Iannantuono,Gregory Emery

Disease markers 2019:9751923 PubMed31007778

2019

KIF23 Promotes Gastric Cancer by Stimulating Cell Proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Long Li,Ya-Ming Ji,Rui Song,Xiao-Ning Li,Lan-Shuan Guo
View all publications
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Product promise

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