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AB235955

Anti-MKLP1 antibody

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(5 Publications)

Rabbit Polyclonal MKLP1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KIF23 aa 850 to C-terminus.

View Alternative Names

KNSL5, MKLP1, KIF23, Kinesin-like protein KIF23, Kinesin-like protein 5, Mitotic kinesin-like protein 1

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MKLP1 antibody (AB235955)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MKLP1 antibody (AB235955)

Paraffin-embedded human kidney tissue stained for MKLP1 using ab235955 at 1/400 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human KIF23 aa 850 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q02241

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MKLP1 also known as mitotic kinesin-like protein 1 is a motor protein with a molecular weight of around 95 kDa. This protein plays an important role in cell division by facilitating the movement of chromosomes and the formation of the spindle apparatus during mitosis. MKLP1 is widely expressed in proliferating cells and tissues especially in those undergoing rapid cell cycles such as embryonic tissues and tumors.
Biological function summary

MKLP1 functions as a component of the centralspindlin complex where it interacts with other proteins to regulate cytoskeletal dynamics. It is critical in the formation and resolution of the central spindle during mitosis ensuring proper separation of cells. By coordinating microtubule bundling and motor activities MKLP1 assists in the maintenance of cell polarity and cell shape changes necessary for mitosis completion.

Pathways

MKLP1 is integral to the mitotic and cell cycle pathways. In particular it is important in the mechanics of the spindle assembly checkpoint which ensures chromosomes are correctly segregated before cell division proceeds. MKLP1 interacts with proteins like Aurora B kinase and ECT2 which regulate the dynamics of cytoskeletal reorganization during these critical phases.

MKLP1 has associations with cancers and neurodevelopmental disorders. In tumors overexpression or mutation of MKLP1 often leads to aneuploidy contributing to tumor progression and metastasis. In the context of neurodevelopmental disorders disruptions in MKLP1 function can relate to conditions such as microcephaly due to its role in cell proliferation. Altered interactions between MKLP1 and proteins like BRCA2 in cancer highlight its relevance in both cell division and stability of the genome.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Essential for cytokinesis in Rho-mediated signaling. Required for the localization of ECT2 to the central spindle. Plus-end-directed motor enzyme that moves antiparallel microtubules in vitro.
See full target information KIF23
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Medicine 101:e29312 PubMed35713434

2022

KIF23 is a potential biomarker of diffuse large B cell lymphoma: Analysis based on bioinformatics and immunohistochemistry.

Applications

Unspecified application

Species

Unspecified reactive species

Yuqi Gong,Lingna Zhou,Liya Ding,Jing Zhao,Zhe Wang,Guoping Ren,Jing Zhang,Zhengrong Mao,Ren Zhou

Annals of translational medicine 8:1394 PubMed33313139

2020

KIF23 enhances cell proliferation in pancreatic ductal adenocarcinoma and is a potent therapeutic target.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Tao Gao,Jin Ren,Jie Yu,Sheng-Nan Li,Xiao-Fan Guo,Yi-Zhang Zhou

Neoplasma 68:298-306 PubMed33231086

2020

Kinesin family member 23 (KIF23) contributes to the progression of bladder cancer cells in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Dong-Wei Yao,Qun Song,Xiao-Zhou He

World journal of gastrointestinal oncology 12:1104-1118 PubMed33133380

2020

Prognostic significance of KIF23 expression in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wei-Tian Liang,Xiao-Fang Liu,Hai-Bo Huang,Zi-Ming Gao,Kai Li

Disease markers 2019:9751923 PubMed31007778

2019

KIF23 Promotes Gastric Cancer by Stimulating Cell Proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Long Li,Ya-Ming Ji,Rui Song,Xiao-Ning Li,Lan-Shuan Guo
View all publications
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