AB1351

Anti-MKP-1 antibody

Name: Anti-MKP-1 antibody (ab1351) | Abcam
Brand: Abcam Limited
SKU: AB1351
Price: 490 USD
Availability: InStock
Rating: 4 (6 reviews)

(6 Reviews)

(11 Publications)

Goat Polyclonal MKP-1 antibody. Suitable for WB and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human DUSP1 aa 350 to C-terminus.

View Alternative Names

CL100, MKP1, PTPN10, VH1, DUSP1, Dual specificity protein phosphatase 1, Dual specificity protein phosphatase hVH1, Mitogen-activated protein kinase phosphatase 1, Protein-tyrosine phosphatase CL100, MAP kinase phosphatase 1, MKP-1

1 Images

Supplier Data

Western blot - Anti-MKP-1 antibody (AB1351)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-MKP-1 antibody (ab1351) at 1 µg/mL

All lanes:

HeLa cell lysate at 35 µg

Predicted band size: 39 kDa

Observed band size: ~40 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human DUSP1 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P28562

Specificity

The immunogen sequence for ab1351, anti MKP-1 antibody (aka DUSP1), shares 100% sequence homology to MKP-2 (aka DUSP4).

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product. We recommend <strong>blocking in milk (3% milk in TBST) for 1 hour at room temperature.</strong></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein known as MKP-1 also called DUSP1 is a dual-specificity phosphatase. It acts mechanically by dephosphorylating the phospho-tyrosine and phospho-serine/threonine residues of its target proteins. The protein has a molecular weight of about 39 kDa. MKP-1 is ubiquitously expressed but shows higher levels in specific tissues such as liver lung and brain where it regulates various cellular processes.

Biological function summary

MKP-1 influences cellular responses to stress and inflammation by inactivating mitogen-activated protein kinases (MAPKs). It does not exist as part of a permanent complex but temporarily interacts with MAPKs. By dephosphorylating these kinases MKP-1 modulates gene expression cell proliferation and apoptosis. Through these interactions the protein plays a significant role in protecting cells from excessive inflammatory signals and stress-induced damage.

Pathways

The MKP-1 protein is integral to the MAPK signaling pathway and the JNK signaling cascade. MKP-1 directly affects the balance of MAPK-related proteins such as ERK JNK and p38 to help regulate various cellular outcomes. By dephosphorylating these proteins MKP-1 acts as a negative feedback regulator ensuring that cellular responses to external stimuli are controlled and do not become harmful.

MKP-1 is associated with inflammatory diseases and cancer. Inflammatory disorders such as rheumatoid arthritis show link with aberrant regulation of MKP-1 as it often interacts with MAPKs involved in inflammation. In cancer altered MKP-1 expression can affect tumor growth and progression due to its regulatory role on cell proliferation pathways. The protein's interaction with key signaling molecules in these diseases highlights its potential as a therapeutic target.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-183' and 'Tyr-185', regulating its activity during the meiotic cell cycle.

See full target information DUSP1

style

left-column

Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Journal of pharmaceutical analysis 15:101169 PubMed40708574

2025

Aldolase A accelerates hepatocarcinogenesis by refactoring c-Jun transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Yang,Guang-Yuan Ma,Xiao-Qiang Li,Na Tang,Yang Sun,Xiao-Wei Hao,Ke-Han Wu,Yu-Bo Wang,Wen Tian,Xin Fan,Zezhi Li,Caixia Feng,Xu Chao,Yu-Fan Wang,Yao Liu,Di Li,Wei Cao

Journal of translational medicine 22:1029 PubMed39548564

2024

Modulation of SRC by SNTB1 activates the Hippo-YAP pathway during colon adenocarcinoma metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengyan Chang,Runzhi Huang,Jiaqi Song,Zhenyu Li,Man Pi,Shuyuan Xian,Jingcheng Zhang,Jinglei Huang,Ruting Xie,Guo Ji,Dongyan Han,Qiongyi Huang

International journal of molecular medicine 47: PubMed33448324

2021

Inhibition of miR‑101‑3p protects against sepsis‑induced myocardial injury by inhibiting MAPK and NF‑κB pathway activation via the upregulation of DUSP1.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Xin,Li Tang,Jing Chen,Dong Chen,Wen Wen,Fugang Han

American journal of physiology. Cell physiology 317:C1183-C1193 PubMed31532716

2019

Dual-specificity phosphatase 1 regulates cell cycle progression and apoptosis in cumulus cells by affecting mitochondrial function, oxidative stress, and autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Xu-Huang Fu,Cheng-Zhen Chen,Sheng Li,Dong-Xu Han,Yi-Jie Wang,Bao Yuan,Yan Gao,Jia-Bao Zhang,Hao Jiang

Carcinogenesis 41:417-429 PubMed31504251

2019

MKP1 phosphatase is recruited by CXCL12 in glioblastoma cells and plays a role in DNA strand breaks repair.

Applications

Unspecified application

Species

Unspecified reactive species

Matthias Dedobbeleer,Estelle Willems,Jeremy Lambert,Arnaud Lombard,Marina Digregorio,Paul Noel Lumapat,Emmanuel Di Valentin,Stephen Freeman,Nicolas Goffart,Felix Scholtes,Bernard Rogister

eLife 7: PubMed30475204

2018

Hyperactivation of ERK by multiple mechanisms is toxic to RTK-RAS mutation-driven lung adenocarcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Arun M Unni,Bryant Harbourne,Min Hee Oh,Sophia Wild,John R Ferrarone,William W Lockwood,Harold Varmus

Molecular medicine reports 16:1472-1478 PubMed29067454

2017

Estradiol postconditioning relieves ischemia/reperfusion injury in axial skin flaps of rats, inhibits apoptosis and alters the MKP-1/ERK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qianheng Jin,Jihui Ju,Lei Xu,Yuefei Liu,Zhimin Li,Yi Fu,Ruixing Hou

Oncotarget 8:12133-12144 PubMed28129656

2017

DUSP1 inhibits cell proliferation, metastasis and invasion and angiogenesis in gallbladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jiliang Shen,Senjun Zhou,Liang Shi,Xiaolong Liu,Hui Lin,Hong Yu, Xiaoliang,Jiacheng Tang,Tunan Yu,Xiujun Cai

Oncogene 33:5675-87 PubMed24317512

2013

CDK/CK1 inhibitors roscovitine and CR8 downregulate amplified MYCN in neuroblastoma cells.

Applications

Unspecified application

Species

Human

C Delehouzé,K Godl,N Loaëc,C Bruyère,N Desban,N Oumata,H Galons,T I Roumeliotis,E G Giannopoulou,J Grenet,D Twitchell,J Lahti,N Mouchet,M-D Galibert,S D Garbis,L Meijer

Endocrinology 151:4882-93 PubMed20685880

2010

Modulation of gonadotropin-releasing hormone-induced extracellular signal-regulated kinase activation by dual-specificity protein phosphatase 1 in LbetaT2 gonadotropes.

Applications

Species

Mouse

Kathryn A Nguyen,Rachel E Intriago,Hiral C Upadhyay,Sharon J Santos,Nicholas J G Webster,Mark A Lawson

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MKP-1 antibody

Western blot - Anti-MKP-1 antibody (AB1351)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise