AB123804

Anti-MKRN1 antibody

Name: Anti-MKRN1 antibody (ab123804) | Abcam
Brand: Abcam Limited
SKU: AB123804
Price: 535 USD
Availability: InStock

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Goat Polyclonal MKRN1 antibody. Suitable for WB and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human MKRN1 aa 100-150.

View Alternative Names

RNF61, MKRN1, E3 ubiquitin-protein ligase makorin-1, RING finger protein 61, RING-type E3 ubiquitin transferase makorin-1

2 Images

Supplier Data

Western blot - Anti-MKRN1 antibody (AB123804)

All lanes:

Western blot - Anti-MKRN1 antibody (ab123804) at 1 µg/mL

Lane 1:

fetal mouse brain tissue lysate at 35 µg

Lane 2:

adult mouse brain tissue lysate at 35 µg

Predicted band size: 53 kDa

Observed band size: 35 kDa,50 kDa

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Unknown

Western blot - Anti-MKRN1 antibody (AB123804)

Lane 1:

Western blot - Anti-MKRN1 antibody (ab123804) at 0.3 µg/mL

Lane 2:

Western blot - Anti-MKRN1 antibody (ab123804) at 1 mg/mL

Lane 3:

anti-DYKDDDDK Tag anntibody at 1/1000 dilution

Lane 1:

HEK293 lysate, over-expressing Human MKRN1 with DYKDDDDK tag in RIPA buffer at 10 µg

Lane 2:

HEK293 lysate, mock-transfected at 10 µg

Lane 3:

HEK293 lysate, over-expressing Human MKRN1 with DYKDDDDK tag in RIPA buffer at 10 µg

Predicted band size: 53 kDa

Observed band size: 60 kDa

true

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Synthetic Peptide within Human MKRN1 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

Q9UHC7

Specificity

ab123804 is expected to recognize both reported isoforms (NP_038474.2; NP_001138597.1).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab123804 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MKRN1 also known as Makorin-1 is a protein that functions as an E3 ubiquitin ligase. This protein has a molecular mass of approximately 58-60 kDa. It is highly expressed in human tissues including the brain heart and skeletal muscle. MKRN1 contains distinct zinc finger motifs that allow it to tether to its substrates aiding in target protein modification and degradation.

Biological function summary

MKRN1 regulates protein stability and turnover within cells. It plays a significant role in controlling gene expression through ubiquitination processes. MKRN1 does not form part of larger protein complexes but interacts individually with its targets. Researchers study its impact on the transcriptional activities of specific proteins given its ability to tag substrates for proteasomal degradation.

Pathways

MKRN1 participates in key cellular pathways such as cell cycle regulation and apoptosis. It interacts with proteins like p53 and ribosomal protein L6 influencing these pathways. Its ubiquitin ligase function connects it to various cellular control mechanisms essential for maintaining homeostasis and responding to cellular stressors.

Researchers established a connection between MKRN1 and cancer specifically prostate cancer. Its regulation of p53 activity influences tumor growth and cell death. Additionally studies show links between MKRN1 and neurodegenerative disorders implicating its role in protein aggregation and neuron survival. MKRN1's interaction with key proteins such as p53 and neuronal factors highlights its potential as a therapeutic target in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/TP53 under normal conditions, but stimulates apoptosis by repressing CDKN1A under stress conditions. Acts as a negative regulator of telomerase. Has negative and positive effects on RNA polymerase II-dependent transcription.

See full target information MKRN1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MKRN1 antibody

Western blot - Anti-MKRN1 antibody (AB123804)

Western blot - Anti-MKRN1 antibody (AB123804)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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