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AB72054

Anti-MKRN1 antibody

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(4 Publications)

Rabbit Polyclonal MKRN1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human MKRN1.

View Alternative Names

RNF61, MKRN1, E3 ubiquitin-protein ligase makorin-1, RING finger protein 61, RING-type E3 ubiquitin transferase makorin-1

2 Images
Immunoprecipitation - Anti-MKRN1 antibody (AB72054)
  • IP

Supplier Data

Immunoprecipitation - Anti-MKRN1 antibody (AB72054)

MGRN1 was immunoprecipitated from HEK-293T cell lysate (1.0 mg per IP reaction; 20% of IP loaded) with ab72054 at 6 μg per reaction. Western blot was performed from the immunoprecipitate with ab72054 at 0.1 μg/ml.

Lane 1 : ab72054 IP in HEK-293T whole cell lysate.
Lane 2 : ab72054 IP in HEK-293T whole cell lysate.
Lane 3 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence

All lanes:

Immunoprecipitation - Anti-MKRN1 antibody (ab72054)

Predicted band size: 53 kDa

false

Exposure time: 3s

Western blot - Anti-MKRN1 antibody (AB72054)
  • WB

Supplier Data

Western blot - Anti-MKRN1 antibody (AB72054)

Detection : Chemiluminescence

All lanes:

Western blot - Anti-MKRN1 antibody (ab72054) at 0.04 µg/mL

Lane 1:

HeLa cell lysate at 50 µg

Lane 2:

HEK293T cell lysate at 50 µg

Lane 3:

Jurkat cell lysate at 50 µg

Lane 4:

TCMK1 cell lysate at 50 µg

Lane 5:

NIH3T3 cell lysate at 50 µg

Predicted band size: 53 kDa

false

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human MKRN1. The exact immunogen used to generate this antibody is proprietary information.

Q9UHC7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MKRN1 also known as Makorin-1 is a protein that functions as an E3 ubiquitin ligase. This protein has a molecular mass of approximately 58-60 kDa. It is highly expressed in human tissues including the brain heart and skeletal muscle. MKRN1 contains distinct zinc finger motifs that allow it to tether to its substrates aiding in target protein modification and degradation.
Biological function summary

MKRN1 regulates protein stability and turnover within cells. It plays a significant role in controlling gene expression through ubiquitination processes. MKRN1 does not form part of larger protein complexes but interacts individually with its targets. Researchers study its impact on the transcriptional activities of specific proteins given its ability to tag substrates for proteasomal degradation.

Pathways

MKRN1 participates in key cellular pathways such as cell cycle regulation and apoptosis. It interacts with proteins like p53 and ribosomal protein L6 influencing these pathways. Its ubiquitin ligase function connects it to various cellular control mechanisms essential for maintaining homeostasis and responding to cellular stressors.

Researchers established a connection between MKRN1 and cancer specifically prostate cancer. Its regulation of p53 activity influences tumor growth and cell death. Additionally studies show links between MKRN1 and neurodegenerative disorders implicating its role in protein aggregation and neuron survival. MKRN1's interaction with key proteins such as p53 and neuronal factors highlights its potential as a therapeutic target in these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/TP53 under normal conditions, but stimulates apoptosis by repressing CDKN1A under stress conditions. Acts as a negative regulator of telomerase. Has negative and positive effects on RNA polymerase II-dependent transcription.
See full target information MKRN1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research 39:238 PubMed33168024

2020

MiRNA-340-5p mediates the functional and infiltrative promotion of tumor-infiltrating CD8 T lymphocytes in human diffuse large B cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yangyang Xu,Zhenchuan Liu,Lixin Lv,Ping Li,Bing Xiu,Wenbin Qian,Aibin Liang

Theranostics 9:4795-4810 PubMed31367258

2019

Hypoxia-induced feedback of HIF-1α and lncRNA-CF129 contributes to pancreatic cancer progression through stabilization of p53 protein.

Applications

Unspecified application

Species

Unspecified reactive species

Mingliang Liu,Jianxin Zhong,Zhu Zeng,Kang Huang,Zeng Ye,Shijiang Deng,Hengyu Chen,Fengyu Xu,Qiang Li,Gang Zhao

EMBO reports 16:1334-57 PubMed26265008

2015

Integrative genomics positions MKRN1 as a novel ribonucleoprotein within the embryonic stem cell gene regulatory network.

Applications

Flow Cyt, WB

Species

Unspecified reactive species, Unspecified reactive species

Paul A Cassar,Richard L Carpenedo,Payman Samavarchi-Tehrani,Jonathan B Olsen,Chang Jun Park,Wing Y Chang,Zhaoyi Chen,Chandarong Choey,Sean Delaney,Huishan Guo,Hongbo Guo,R Matthew Tanner,Theodore J Perkins,Scott A Tenenbaum,Andrew Emili,Jeffrey L Wrana,Derrick Gibbings,William L Stanford

Science signaling 8:ra58 PubMed26082434

2015

Binding of the sphingolipid S1P to hTERT stabilizes telomerase at the nuclear periphery by allosterically mimicking protein phosphorylation.

Applications

WB

Species

Human

Shanmugam Panneer Selvam,Ryan M De Palma,Joshua J Oaks,Natalia Oleinik,Yuri K Peterson,Robert V Stahelin,Emmanuel Skordalakes,Suriyan Ponnusamy,Elizabeth Garrett-Mayer,Charles D Smith,Besim Ogretmen
View all publications
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