AB72055

Anti-MKRN2 antibody

Name: Anti-MKRN2 antibody (ab72055) | Abcam
Brand: Abcam Limited
SKU: AB72055

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(3 Publications )

Rabbit Polyclonal MKRN2 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human MKRN2.

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MKRN2 antibody (AB72055)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MKRN2 antibody (AB72055)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MKRN2 with ab72055 at 1/1000 (1µg/ml). Detection : DAB.

Unknown

Immunoprecipitation - Anti-MKRN2 antibody (AB72055)

Detection of Human MKRN2 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72055 at 3 µg/mg lysate (Lane 1). Lane 2 represents Control IgG IP. Subsequent Western blot detection was done using ab72055 at 1 µg/ml.

All lanes:

Immunoprecipitation - Anti-MKRN2 antibody (ab72055)

Predicted band size: 47 kDa

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Unknown

Western blot - Anti-MKRN2 antibody (AB72055)

All lanes:

Western blot - Anti-MKRN2 antibody (ab72055) at 0.1 µg/mL

Lane 1:

Whole cell lysate from HeLa cells at 50 µg

Lane 2:

Whole cell lysate from HeLa cells at 15 µg

Lane 3:

Whole cell lysate from HeLa cells at 5 µg

Lane 4:

Whole cell lysate from 293T cells at 50 µg

Lane 5:

Whole cell lysate from NIH/3T3 cells at 50 µg

Predicted band size: 47 kDa

Observed band size: 38 kDa,51 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, IP, WB

applications

Immunogen

Synthetic Peptide within Human MKRN2. The exact immunogen used to generate this antibody is proprietary information.

Q9H000

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/5000", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chinese hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Ferret": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MKRN2 also known as Makorin ring finger protein 2 functions mechanically as an E3 ubiquitin ligase. It possesses a molecular mass of approximately 49 kDa. This protein contributes to the ubiquitination process by tagging specific substrate proteins for degradation. MKRN2 is expressed broadly across various tissues with higher levels observed in neural tissues. This suggests its role in neural functions although it is not exclusive to the nerves.

Biological function summary

MKRN2 participates in numerous cellular functions through its role in ubiquitination. Its presence in neural tissues indicates a significant involvement in neurodevelopmental processes and neural signaling. MKRN2 interacts as part of a complex with other proteins that mediate protein stability and degradation. This interaction highlights its multifunctional role in cellular homeostasis suggesting its influence extends beyond simple protein tagging.

Pathways

MKRN2 engages in critical signaling cascades such as the Wnt and PI3K/Akt pathways. Within the Wnt pathway MKRN2 can modulate the stability of proteins involved in signal transmission such as β-catenin. The PI3K/Akt pathway involves MKRN2 in regulation of cell survival and growth often linking it with other proteins like PTEN. These pathways indicate MKRN2’s broader importance in cell proliferation and apoptosis control.

MKRN2 has connections with cancer and neurodegenerative diseases. Mutations or dysregulation of MKRN2 can lead to aberrant protein degradation contributing to tumorigenesis and progression of cancers. The protein also shows associations with neurodegenerative disorders through its interaction with substrates involved in neural functioning. Another protein linked to these disorders includes p53 where MKRN2 can influence cell cycle regulation and stress responses in the neural context.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins (By similarity). Promotes the polyubiquitination and proteasome-dependent degradation of RELA/p65, thereby suppressing RELA-mediated NF-kappaB transactivation and negatively regulating inflammatory responses (By similarity). Plays a role in the regulation of spermiation and in male fertility (By similarity).

See full target information MKRN2

Alternative Names

RNF62, HSPC070, MKRN2, E3 ubiquitin-protein ligase makorin-2, RING finger protein 62, RING-type E3 ubiquitin transferase makorin-2

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Publications (3)

Recent publications for all applications. Explore the full listand refine your search

The Journal of cell biology 220: PubMed33507233

2021

The deubiquitylase USP9X controls ribosomal stalling.

Applications

Unspecified application

Species

Unspecified reactive species

Anne Clancy,Claire Heride,Adán Pinto-Fernández,Hannah Elcocks,Andreas Kallinos,Katherine J Kayser-Bricker,Weiping Wang,Victoria Smith,Simon Davis,Shawn Fessler,Crystal McKinnon,Marie Katz,Tim Hammonds,Neil P Jones,Jonathan O'Connell,Bruce Follows,Steven Mischke,Justin A Caravella,Stephanos Ioannidis,Christopher Dinsmore,Sunkyu Kim,Axel Behrens,David Komander,Benedikt M Kessler,Sylvie Urbé,Michael J Clague

Journal of experimental & clinical cancer research 39:238 PubMed33168024

2020

MiRNA-340-5p mediates the functional and infiltrative promotion of tumor-infiltrating CD8 T lymphocytes in human diffuse large B cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yangyang Xu,Zhenchuan Liu,Lixin Lv,Ping Li,Bing Xiu,Wenbin Qian,Aibin Liang

Cell reports 31:107693 PubMed32460013

2020

MKRN2 Physically Interacts with GLE1 to Regulate mRNA Export and Zebrafish Retinal Development.

Applications

Unspecified application

Species

Unspecified reactive species

Eric J Wolf,Amanda Miles,Eliza S Lee,Syed Nabeel-Shah,Jack F Greenblatt,Alexander F Palazzo,Vincent Tropepe,Andrew Emili

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MKRN2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MKRN2 antibody (AB72055)

Immunoprecipitation - Anti-MKRN2 antibody (AB72055)

Western blot - Anti-MKRN2 antibody (AB72055)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (3)

Product promise