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AB177203

Anti-MKRN3 antibody

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(4 Publications)

Rabbit Polyclonal MKRN3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MKRN3 aa 250-450.

View Alternative Names

D15S9, RNF63, ZNF127, MKRN3, Probable E3 ubiquitin-protein ligase makorin-3, RING finger protein 63, RING-type E3 ubiquitin transferase makorin-3, Zinc finger protein 127

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MKRN3 antibody (AB177203)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MKRN3 antibody (AB177203)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human fetal colon tissue labeling MKRN3 with ab177203 at 1/100 dilution.

Western blot - Anti-MKRN3 antibody (AB177203)
  • WB

Supplier Data

Western blot - Anti-MKRN3 antibody (AB177203)

All lanes:

Western blot - Anti-MKRN3 antibody (ab177203) at 1/1000 dilution

All lanes:

A431 cell lysate

Predicted band size: 56 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human MKRN3 aa 250-450. The exact immunogen used to generate this antibody is proprietary information.

Q13064

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Lyophilized
Reconstitution
reconstitute with water at 200µL
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MKRN3 also known as Makorin Ring Finger Protein 3 is an important protein characterized by its E3 ubiquitin ligase activity. This protein has a mass of approximately 43 kDa. MKRN3 is expressed in the hypothalamus and is highly relevant during developmental stages before puberty. It interacts with RNA-binding proteins and other molecular components within cells indicating its mechanistic action in post-translational modification and regulation of cellular processes.
Biological function summary

MKRN3 regulates the puberty onset. It is a part of an intricate network controlling gene expression related to this process. MKRN3 functions as a negative regulator and suppresses the release of puberty-activating signals. This places it within a tightly coordinated biological context where it collaborates with various factors to maintain the prepubertal state. Researchers identify it as an essential player in the hypothalamic-pituitary-gonadal axis.

Pathways

Several cascading signals are affected by MKRN3. It plays a role in the reproductive endocrine axis specifically influencing the GnRH (Gonadotropin-Releasing Hormone) signaling pathway. MKRN3's involvement in these pathways links it with other associated proteins like KISS1 and its receptor which play significant roles in triggering puberty. The regulatory capacity of MKRN3 means it acts as an upstream modulator within these signaling mechanisms.

MKRN3 mutations lead to central precocious puberty. This condition involves early activation of the hypothalamic-pituitary-gonadal axis causing early puberty onset. Additionally altered MKRN3 expression may relate to hypogonadotropic hypogonadism a disorder characterized by delayed or absent puberty. In this context MKRN3 connects with genes and proteins such as GNRHR and TAC3 known for their roles in reproductive development disorders making it necessary for scientists to explore MKRN3's potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins.
See full target information MKRN3
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell communication and signaling : CCS 23:325 PubMed40619404

2025

The scaffold protein DLG4 facilitates RNF63-mediated ubiquitination and degradation of STAT3 in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shisong Chen,Hongjie Xu,Ning Li,Yang Yang,Ruxi Pang,Shuwei Zhang,Junjie Qiao,Hao Chen

The Journal of experimental medicine 218: PubMed34143182

2021

E3 ligase MKRN3 is a tumor suppressor regulating PABPC1 ubiquitination in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Li,Xufen Zheng,Hua Tang,Yuan-Sheng Zang,Chunling Zeng,Xiaoxiao Liu,Yanying Shen,Yuzhi Pang,Simin Wang,Feifei Xie,Xiaojing Lu,Yuxiang Luo,Zhang Li,Wenbo Bi,Xiaona Jia,Tao Huang,Rongqiang Wei,Kenan Huang,Zihao Chen,Qingchen Zhu,Yi He,Miaoying Zhang,Zhizhan Gu,Yichuan Xiao,Xiaoyang Zhang,Jonathan A Fletcher,Yuexiang Wang

Frontiers in cell and developmental biology 9:606106 PubMed33816462

2021

Identification of Biomarkers Related to CD8 T Cell Infiltration With Gene Co-expression Network in Lung Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Min Tang,Yukun Li,Xianyu Luo,Jiao Xiao,Juan Wang,Xin Zeng,Qihao Hu,Xiaoyan Chen,Si-Jie Tan,Jun Hu

Frontiers in endocrinology 10:677 PubMed31636607

2019

Central Precocious Puberty Caused by Novel Mutations in the Promoter and 5'-UTR Region of the Imprinted Gene.

Applications

Unspecified application

Species

Unspecified reactive species

Pavlos Fanis,Nicos Skordis,Meropi Toumba,Nikoletta Papaioannou,Anestis Makris,Andreas Kyriakou,Vassos Neocleous,Leonidas A Phylactou
View all publications
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