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AB236400

Anti-MKX antibody [OTI6A12]

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(2 Publications)

Mouse Monoclonal MKX antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MKX.

View Alternative Names

C10orf48, IRXL1, MKX, Homeobox protein Mohawk

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MKX antibody [OTI6A12] (AB236400)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MKX antibody [OTI6A12] (AB236400)

Paraffin-embedded human prostate tissue stained for MKX using ab236400 at 1/150 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MKX antibody [OTI6A12] (AB236400)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MKX antibody [OTI6A12] (AB236400)

Paraffin-embedded human prostate carcinoma tissue stained for MKX using ab236400 at 1/150 dilution in immunohistochemical analysis.

Western blot - Anti-MKX antibody [OTI6A12] (AB236400)
  • WB

Supplier Data

Western blot - Anti-MKX antibody [OTI6A12] (AB236400)

All lanes:

Western blot - Anti-MKX antibody [OTI6A12] (ab236400) at 1/2000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA for 48 hrs at 5 µg

Lane 2:

HEK-293T cell lysate transfected with pCMV6-ENTRY MKX cDNA for 48 hrs at 5 µg

Predicted band size: 39 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI6A12

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human MKX.

Q8IYA7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p>Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 minutes.</p>" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Clone OTI6A12 (formerly 6A12).
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified from cell culture supernatant.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MKX also known as Mohawk Homeobox is a transcription factor with a mass of approximately 36 kDa. This protein plays a role in gene regulation by binding to DNA sequences and influencing the expression of target genes. MKX mainly expresses in mesodermal tissues especially in skeletal muscles tendons and ligaments during development. Its expression is spatially and temporally regulated reflecting its involvement in tissue-specific processes.
Biological function summary

MKX influences the formation and maintenance of fibrous connective tissues. It does not function as part of a known complex but interacts with other transcription factors to regulate gene expression patterns essential for tissue differentiation and function. MKX is involved in orchestrating the development of musculoskeletal structures by modulating the expression of genes related to extracellular matrix composition and organization. Through these actions MKX contributes to the structural integrity and mechanical properties of tendons and ligaments.

Pathways

MKX plays a role in the tendon development and TGF-β signaling pathways. In these pathways MKX coordinates with proteins such as Scleraxis (SCX) and Tenomodulin (TNMD) to promote tendon and ligament differentiation. The interaction of MKX within these pathways facilitates cellular processes that mediate tissue formation and repair indicating its integral role in connective tissue physiology.

MKX has strong connections to conditions involving tendon and ligament degeneration such as tendinopathy and osteoarthritis. Altered expression levels or mutations in MKX may disrupt normal tissue development and repair processes linking it to these disorders. Furthermore MKX interacts with proteins like Matrix Metalloproteinases (MMPs) which play a role in tissue remodeling and are involved in pathological conditions when dysregulated. Understanding the functions and interactions of MKX aids in exploring therapeutic targets for musculoskeletal diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May act as a morphogenetic regulator of cell adhesion.
See full target information MKX
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of tissue engineering 16:20417314251315185 PubMed39882545

2025

Therapeutic potential and mechanisms of umbilical cord mesenchymal stem cells differentiating into tendon cells and promotion of rotator cuff tendon-bone healing.

Applications

Unspecified application

Species

Unspecified reactive species

Youliang Shen,Yuelei Wang,Yidan Xu,Jie Wang,Chuqiang Yin,Zengshuai Han,Feng Shen,Ting Wang

Aging 15:3807-3825 PubMed37166429

2023

Identification and validation of metabolism-related genes signature and immune infiltration landscape of rheumatoid arthritis based on machine learning.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaoyang Guo,Yuanye Ma,Yaqing Wang,Hongfei Xiang,Huifei Cui,Zuoran Fan,Youfu Zhu,Dongming Xing,Bohua Chen,Hao Tao,Zhu Guo,Xiaolin Wu
View all publications
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