AB186436

Anti-MLC1 antibody [EPR10942(2)] - N-terminal

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal MLC1 antibody. N-terminal. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

KIAA0027, WKL1, MLC1, Membrane protein MLC1, Megalencephalic leukoencephalopathy with subcortical cysts protein 1

2 Images

Supplier Data

Western blot - Anti-MLC1 antibody [EPR10942(2)] - N-terminal (AB186436)

The observed MW is lower than the predicted 41kDa, which may be due to the high hydrophobicity of the protein.

All lanes:

Western blot - Anti-MLC1 antibody [EPR10942(2)] - N-terminal (ab186436) at 1/10000 dilution

All lanes:

Human hypothalamus lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 41 kDa

false

Supplier Data

Western blot - Anti-MLC1 antibody [EPR10942(2)] - N-terminal (AB186436)

The observed MW is lower than the predicted 41kDa, which may be due to the high hydrophobicity of the protein.

All lanes:

Western blot - Anti-MLC1 antibody [EPR10942(2)] - N-terminal (ab186436) at 1/1000 dilution

All lanes:

Human hippocampus lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 41 kDa

false

Carrier free

Anti-MLC1 antibody [EPR10942(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10942(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MLC1 also known as the Megalencephalic Leukoencephalopathy with subcortical Cysts 1 protein is a membrane protein with a molecular mass of approximately 24 kDa. It expresses mainly in the brain especially in astrocytes and oligodendrocytes. MLC1 localizes in the perivascular astrocytic processes which indicates its involvement in maintaining ion homeostasis in the brain. Its primary function revolves around cellular processes related to water and ion transport.

Biological function summary

The MLC1 protein is linked to the regulation of ion channels and is part of a larger protein complex on the astrocyte cell membranes. This complex plays a role in the astrocytic response to osmotic stress contributing to cellular volume regulation. MLC1 influences critical cellular mechanisms through its interactions with other proteins in the complex helping to stabilize ion concentrations and supporting proper brain function under various stress conditions.

Pathways

MLC1 contributes significantly to the osmoregulatory signaling pathways and modulates the astrocyte-neuron communication network. It has interactions with proteins like GlialCAM and ClC-2 which are essential components of the regulatory pathways controlling astrocyte ion homeostasis. These pathways ensure the maintenance of electrochemical gradients important for neuronal signaling and overall brain homeostasis.

MLC1 links directly to megalencephalic leukoencephalopathy with subcortical cysts (MLC) a rare neurodegenerative disorder. Mutations in the MLC1 gene often result in dysfunctional protein which leads to improper ion and water balance in the brain. This condition associates with cognitive and motor deficits. It also connects with the GlialCAM protein which can exacerbate or contribute to the development of MLC when mutated highlighting the importance of the MLC1-GlialCAM interaction in maintaining brain health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transmembrane protein mainly expressed in brain atrocytes that may play a role in transport across the blood-brain and brain-cerebrospinal fluid barriers (PubMed : 22328087). Regulates the response of astrocytes to hypo-osmosis by promoting calcium influx (PubMed : 22328087). May function as regulatory protein of membrane protein complexes such as ion channels (Probable).

See full target information MLC1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 8:12389 PubMed30120299

2018

Transcriptional network analysis of human astrocytic endfoot genes reveals region-specific associations with dementia status and tau pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew J Simon,Marie X Wang,Charles F Murchison,Natalie E Roese,Erin L Boespflug,Randall L Woltjer,Jeffrey J Iliff

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MLC1 antibody [EPR10942(2)] - N-terminal

Western blot - Anti-MLC1 antibody [EPR10942(2)] - N-terminal (AB186436)

Western blot - Anti-MLC1 antibody [EPR10942(2)] - N-terminal (AB186436)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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Product promise