AB167169

Anti-MLD antibody [EPR9681]

Name: Anti-MLD antibody [EPR9681] (ab167169) | Abcam
Brand: Abcam Limited
SKU: AB167169
Price: 4915 USD
Availability: InStock
Rating: 1 (2 reviews)

RabMAb
Recombinant
KO Validated
What is this?

(2 Reviews)

(9 Publications)

Rabbit Recombinant Monoclonal MLD antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 9 publications.

View Alternative Names

DES1, MLD, MIG15, DEGS1, Sphingolipid delta(4)-desaturase DES1, Cell migration-inducing gene 15 protein, Degenerative spermatocyte homolog 1, Dihydroceramide desaturase-1, Membrane lipid desaturase, Retinol isomerase

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLD antibody [EPR9681] (AB167169)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLD antibody [EPR9681] (AB167169)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling MLD with ab167169 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-MLD antibody [EPR9681] (AB167169)

All lanes:

Western blot - Anti-MLD antibody [EPR9681] (ab167169) at 1/1000 dilution

Lane 1:

293T cell lysates at 10 µg

Lane 2:

T47D cell lysates at 10 µg

Lane 3:

HepG2 cell lysates at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 38 kDa

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Lab

Western blot - Anti-MLD antibody [EPR9681] (AB167169)

Lanes 1-4 : Merged signal (red and green). Green - ab167169 observed at 38 kDa. Red - loading control ab8245 observed at 36 kDa.

ab167169 Anti-MLD antibody [EPR9681] was shown to specifically react with MLD in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266481 (knockout cell lysate ab257918) was used. Wild-type and MLD knockout samples were subjected to SDS-PAGE. ab167169 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-MLD antibody [EPR9681] (ab167169) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Lane 2:

DEGS1 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Lane 2:

Western blot - Human DEGS1 (MLD) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-degs1-mld-knockout-hek-293t-cell-line-ab266481'>ab266481</a>)

Lane 3:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 20 µg

Lane 4:

T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 38 kDa

Observed band size: 38 kDa

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Carrier free

Anti-MLD antibody [EPR9681] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9681

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MLD also known as Metachromatic Leukodystrophy protein is an enzyme of significant importance in cellular function. This protein has a molecular mass of around 50 kDa. It is primarily expressed in the brain but is also found in peripheral tissues. MLD breaks down sulfatides which are important components of myelin sheaths. The myelin sheaths encapsulate nerve cells and are essential for efficient nerve signal transmission.

Biological function summary

Metachromatic Leukodystrophy protein plays an important role in the maintenance of the nervous system structure and function. It is involved in the degradation of certain lipid compounds to prevent toxic accumulation. When the function of the MLD protein is impaired sulfatides accumulate leading to cellular damage and functional deficits. MLD does not generally function as part of a larger complex; instead it acts independently within its cellular environment.

Pathways

Metachromatic Leukodystrophy protein is integral to the sphingolipid degradation pathway. This pathway includes the catabolism of complex lipid molecules preventing harmful buildup within cells. Another enzyme arylsulfatase A closely interacts with the MLD protein and participates in the same pathway further demonstrating its importance in managing cellular lipids and maintaining cell health.

Metachromatic Leukodystrophy protein is directly related to Metachromatic Leukodystrophy a genetic disorder affecting the nervous system. This disease results from mutations in the gene that encodes for the MLD protein leading to demyelination and neurological decline. Other proteins like cerebroside sulfate become dysregulated in this disorder indicating a complex network of biomolecular interactions disrupted when MLD function is compromised.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Has sphingolipid-delta-4-desaturase activity. Converts D-erythro-sphinganine to D-erythro-sphingosine (E-sphing-4-enine) (PubMed : 11937514, PubMed : 30620337, PubMed : 30620338). Catalyzes the equilibrium isomerization of retinols (By similarity).

See full target information DEGS1

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Journal of lipid research 65:100517 PubMed38342436

2024

Loss of function and reduced levels of sphingolipid desaturase DEGS1 variants are both relevant in disease mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Michele Dei Cas,Linda Montavoci,Claudia Pasini,Anna Caretti,Sara Penati,Carla Martinelli,Umberto Gianelli,Sara Casati,Francesca Nardecchia,Annalaura Torella,Nicola Brunetti-Pierri,Marco Trinchera

JCI insight 8: PubMed36927688

2023

Upregulation of acid ceramidase contributes to tumor progression in tuberous sclerosis complex.

Applications

Unspecified application

Species

Unspecified reactive species

Aristotelis Astrinidis,Chenggang Li,Erik Y Zhang,Xueheng Zhao,Shuyang Zhao,Minzhe Guo,Tasnim Olatoke,Ushodaya Mattam,Rong Huang,Alan G Zhang,Lori Pitstick,Elizabeth J Kopras,Nishant Gupta,Roman Jandarov,Eric P Smith,Elizabeth Fugate,Diana Lindquist,Maciej M Markiewski,Magdalena Karbowniczek,Kathryn A Wikenheiser-Brokamp,Kenneth D R Setchell,Francis X McCormack,Yan Xu,Jane J Yu

Biological & pharmaceutical bulletin 45:1559-1563 PubMed36184516

2022

Dihydroceramide Δ4-Desaturase 1 Is Not Involved in SARS-CoV-2 Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Yasuhiro Hayashi,Kouki Matsuda,Kazunari Tanigawa,Takashi Tanikawa,Kenji Maeda,Kiyoto Tsuchiya

Clinical and translational medicine 12:e810 PubMed35560527

2022

Elevation of fatty acid desaturase 2 in esophageal adenocarcinoma increases polyunsaturated lipids and may exacerbate bile acid-induced DNA damage.

Applications

Unspecified application

Species

Unspecified reactive species

Jeffrey Molendijk,Cathryn M Kolka,Henry Cairns,Sandra Brosda,Ahmed Mohamed,Alok K Shah,Ian Brown,Mark P Hodson,Thomas Hennessy,Guanghao Liu,Thomas Stoll,Renee S Richards,Michael Gartside,Kalpana Patel,Nicholas J Clemons,Wayne A Phillips,Andrew Barbour,Johan A Westerhuis,Michelle M Hill

iScience 24:103437 PubMed34877496

2021

Dihydroceramide desaturase promotes the formation of intraluminal vesicles and inhibits autophagy to increase exosome production.

Applications

Unspecified application

Species

Unspecified reactive species

Chen-Yi Wu,Jhih-Gang Jhang,Wan-Syuan Lin,Pei-Huan Chuang,Chih-Wei Lin,Li-An Chu,Ann-Shyn Chiang,Han-Chen Ho,Chih-Chiang Chan,Shu-Yi Huang

Autophagy 17:1349-1366 PubMed32397857

2020

The anti-cancer drug ABTL0812 induces ER stress-mediated cytotoxic autophagy by increasing dihydroceramide levels in cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Pau Muñoz-Guardiola,Josefina Casas,Elisabet Megías-Roda,Sònia Solé,Héctor Perez-Montoyo,Marc Yeste-Velasco,Tatiana Erazo,Nora Diéguez-Martínez,Sergio Espinosa-Gil,Cristina Muñoz-Pinedo,Guillermo Yoldi,Jose L Abad,Miguel F Segura,Teresa Moran,Margarita Romeo,Joaquim Bosch-Barrera,Ana Oaknin,Jose Alfón,Carles Domènech,Gemma Fabriàs,Guillermo Velasco,Jose M Lizcano

Journal of lipid research 60:1225-1235 PubMed30988134

2019

Genetic and pharmacological inhibition of acid ceramidase prevents asymmetric cell division by neosis.

Applications

Unspecified application

Species

Unspecified reactive species

Shai White-Gilbertson,Ping Lu,James S Norris,Christina Voelkel-Johnson

The Journal of clinical investigation 129:1229-1239 PubMed30620338

2019

DEGS1-associated aberrant sphingolipid metabolism impairs nervous system function in humans.

Applications

Unspecified application

Species

Unspecified reactive species

Gergely Karsai,Florian Kraft,Natja Haag,G Christoph Korenke,Benjamin Hänisch,Alaa Othman,Saranya Suriyanarayanan,Regula Steiner,Cordula Knopp,Michael Mull,Markus Bergmann,J Michael Schröder,Joachim Weis,Miriam Elbracht,Matthias Begemann,Thorsten Hornemann,Ingo Kurth

Journal of lipid research 60:819-831 PubMed30573560

2018

Multiple actions of doxorubicin on the sphingolipid network revealed by flux analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Justin M Snider,Magali Trayssac,Christopher J Clarke,Nicholas Schwartz,Ashley J Snider,Lina M Obeid,Chiara Luberto,Yusuf A Hannun

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MLD antibody [EPR9681]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLD antibody [EPR9681] (AB167169)

Western blot - Anti-MLD antibody [EPR9681] (AB167169)

Western blot - Anti-MLD antibody [EPR9681] (AB167169)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Product promise