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Rabbit Recombinant Monoclonal MLD antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 8 publications.


Images

Western blot - Anti-MLD antibody [EPR9681] (AB167169), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLD antibody [EPR9681] (AB167169), expandable thumbnail
  • Western blot - Anti-MLD antibody [EPR9681] (AB167169), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWB
Human
Tested
Not recommended
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Has sphingolipid-delta-4-desaturase activity. Converts D-erythro-sphinganine to D-erythro-sphingosine (E-sphing-4-enine) (PubMed:11937514, PubMed:30620337, PubMed:30620338). Catalyzes the equilibrium isomerization of retinols (By similarity).

Alternative names

DES1, MLD, MIG15, DEGS1, Sphingolipid delta(4)-desaturase DES1, Cell migration-inducing gene 15 protein, Degenerative spermatocyte homolog 1, Dihydroceramide desaturase-1, Membrane lipid desaturase, Retinol isomerase

Recommended products

Rabbit Recombinant Monoclonal MLD antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 8 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR9681
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

MLD also known as Metachromatic Leukodystrophy protein is an enzyme of significant importance in cellular function. This protein has a molecular mass of around 50 kDa. It is primarily expressed in the brain but is also found in peripheral tissues. MLD breaks down sulfatides which are important components of myelin sheaths. The myelin sheaths encapsulate nerve cells and are essential for efficient nerve signal transmission.

Biological function summary

Metachromatic Leukodystrophy protein plays an important role in the maintenance of the nervous system structure and function. It is involved in the degradation of certain lipid compounds to prevent toxic accumulation. When the function of the MLD protein is impaired sulfatides accumulate leading to cellular damage and functional deficits. MLD does not generally function as part of a larger complex; instead it acts independently within its cellular environment.

Pathways

Metachromatic Leukodystrophy protein is integral to the sphingolipid degradation pathway. This pathway includes the catabolism of complex lipid molecules preventing harmful buildup within cells. Another enzyme arylsulfatase A closely interacts with the MLD protein and participates in the same pathway further demonstrating its importance in managing cellular lipids and maintaining cell health.

Associated diseases and disorders

Metachromatic Leukodystrophy protein is directly related to Metachromatic Leukodystrophy a genetic disorder affecting the nervous system. This disease results from mutations in the gene that encodes for the MLD protein leading to demyelination and neurological decline. Other proteins like cerebroside sulfate become dysregulated in this disorder indicating a complex network of biomolecular interactions disrupted when MLD function is compromised.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-MLD antibody [EPR9681] (ab167169), expandable thumbnail

    Western blot - Anti-MLD antibody [EPR9681] (ab167169)

    Lanes 1-4: Merged signal (red and green). Green - ab167169 observed at 38 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

    ab167169 Anti-MLD antibody [EPR9681] was shown to specifically react with MLD in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line Human DEGS1 (MLD) knockout HEK-293T cell line ab266481 (knockout cell lysate Human DEGS1 (MLD) knockout HEK-293T cell lysate ab257918) was used. Wild-type and MLD knockout samples were subjected to SDS-PAGE. ab167169 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-MLD antibody [EPR9681] (ab167169) at 1/1000 dilution

    Lane 1: Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

    Lane 2: DEGS1 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

    Lane 2: Western blot - Human DEGS1 (MLD) knockout HEK-293T cell line (Human DEGS1 (MLD) knockout HEK-293T cell line ab266481)

    Lane 3: U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 20 µg

    Lane 4: T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Predicted band size: 38 kDa

    Observed band size: 38 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLD antibody [EPR9681] (ab167169), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLD antibody [EPR9681] (ab167169)

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling MLD with ab167169 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-MLD antibody [EPR9681] (ab167169), expandable thumbnail

    Western blot - Anti-MLD antibody [EPR9681] (ab167169)

    All lanes: Western blot - Anti-MLD antibody [EPR9681] (ab167169) at 1/1000 dilution

    Lane 1: 293T cell lysates at 10 µg

    Lane 2: T47D cell lysates at 10 µg

    Lane 3: HepG2 cell lysates at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 38 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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