AB180184

Anti-MLF2 antibody [EPR10245(2)] - C-terminal

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Recombinant Monoclonal MLF2 antibody. C-terminal. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

Myeloid leukemia factor 2, Myelodysplasia-myeloid leukemia factor 2, MLF2

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLF2 antibody [EPR10245(2)] - C-terminal (AB180184)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLF2 antibody [EPR10245(2)] - C-terminal (AB180184)

Immunohistochemical analysis of paraffin embedded Human prostate tissue labeling MLF2 with ab180184 at 1/50.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-MLF2 antibody [EPR10245(2)] - C-terminal (AB180184)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-MLF2 antibody [EPR10245(2)] - C-terminal (AB180184)

Intracellular flow cytometrical analysis of permeabilized Jurkat cells labeling MLF2 with ab180184 at 1/10 (red) or a rabbit IgG negative (green).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLF2 antibody [EPR10245(2)] - C-terminal (AB180184)

Immunohistochemical analysis of paraffin embedded Human colon tissue labeling MLF2 with ab180184 at 1/50.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-MLF2 antibody [EPR10245(2)] - C-terminal (AB180184)

All lanes:

Western blot - Anti-MLF2 antibody [EPR10245(2)] - C-terminal (ab180184) at 1/1000 dilution

Lane 1:

Jurkat lysate at 10 µg

Lane 2:

Fetal brain tissue lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10245(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MLF2 also known as Myeloid Leukemia Factor 2 is a protein with an approximate mass of 35 kDa. It shows expression mainly in the nucleus and cytoplasm of cells. MLF2 takes part in the regulation of various cellular processes especially those involving cell growth and differentiation. Understanding its activity and expression pattern can help in dissecting its role in cellular function and disease.

Biological function summary

MLF2 contributes to cells' response to stress making it significant in maintaining cellular homeostasis. MLF2 can interact with other proteins to form complexes that regulate transcriptional processes. This regulation is important for normal cellular function and indicates that MLF2 has an influence on gene expression modulation impacting how the cell responds to different stimuli.

Pathways

MLF2 engages in the JAK/STAT signaling pathway which is important in processes like cell proliferation differentiation and apoptosis. It also interacts with the NF-kB signaling pathway important for immune response and inflammation regulation. Through these pathways MLF2 shares connections with proteins such as STAT3 and NF-kB indicating its integration into a network that maintains cellular activity and integrity.

MLF2 associates with cancer and myelodysplastic syndromes. Its altered expression or dysfunction can contribute to tumorigenesis due to its role in cell proliferation and differentiation pathways. In these disease contexts MLF2's interaction with proteins like JAK2 becomes critical as disruptions in these interactions may exacerbate disease progression. Understanding these connections helps in considering MLF2 as a potential therapeutic target in disease treatment strategies.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

See full target information MLF2

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Redox biology 51:102258 PubMed35189550

2022

Redox-dependent loss of flavin by mitochondria complex I is different in brain and heart.

Applications

Unspecified application

Species

Unspecified reactive species

Belem Yoval-Sánchez,Fariha Ansari,Joel James,Zoya Niatsetskaya,Sergey Sosunov,Peter Filipenko,Irina G Tikhonova,Vadim Ten,Ilka Wittig,Ruslan Rafikov,Alexander Galkin

Biochemical and biophysical research communications 529:127-132 PubMed32703400

2020

CRL4 and USP11 oppositely regulate the stability of myeloid leukemia factors (MLFs).

Applications

Unspecified application

Species

Unspecified reactive species

Daoyuan Huang,Cheng Liu,Xiwen Sun,Xinpei Sun,Yanan Qu,Yunyi Tang,Guodong Li,Tanjun Tong

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MLF2 antibody [EPR10245(2)] - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLF2 antibody [EPR10245(2)] - C-terminal (AB180184)

Flow Cytometry (Intracellular) - Anti-MLF2 antibody [EPR10245(2)] - C-terminal (AB180184)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLF2 antibody [EPR10245(2)] - C-terminal (AB180184)

Western blot - Anti-MLF2 antibody [EPR10245(2)] - C-terminal (AB180184)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Complementary Products

Primary Antibodies

Primary Antibodies

Proteins & Peptides

Primary Antibodies

Product promise