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Rabbit Recombinant Monoclonal MLH1 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.


Images

Western blot - Anti-MLH1 antibody [EPR3893] (AB108622), expandable thumbnail
  • Western blot - Anti-MLH1 antibody [EPR3893] (AB108622), expandable thumbnail
  • Western blot - Anti-MLH1 antibody [EPR3893] (AB108622), expandable thumbnail
  • Western blot - Anti-MLH1 antibody [EPR3893] (AB108622), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-P
Human
Not recommended
Not recommended
Tested
Not recommended
Mouse
Not recommended
Not recommended
Expected
Not recommended
Rat
Not recommended
Not recommended
Expected
Not recommended

Not recommended
Not recommended

Species

Mouse, Human, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Human, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

1/1000 - 1/10000

Notes

-

Species

Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Human, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal MLH1 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR3893

Purity

Tissue culture supernatant

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

Notes

To see more of the key markers and tools you need to study the hallmarks of cancer, including genome instability and mutation, please visit the following page.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

MLH1 also known as MutL homolog 1 is a protein involved in DNA mismatch repair an important mechanism for maintaining genetic stability. It has a molecular weight of approximately 87 kDa. This protein is expressed in various tissues but is most abundant in the colonic epithelium and endometrium. MLH1 acts mechanically by forming heterodimers with other proteins collaborating in correcting errors that occur during DNA replication.

Biological function summary

The function of MLH1 involves its role in the mismatch repair (MMR) system. It is part of a complex with PMS2 forming a heterodimer known as MutLα which is essential for the repair process. This complex scans newly synthesized DNA for mispaired bases and initiates repair preserving genomic integrity. The proper function of MLH1 and its interaction with PMS2 ensures that DNA replication errors do not accumulate and cause harmful mutations.

Pathways

MLH1 operates within the mismatch repair pathway and interacts closely with MLH3 and PMS2 proteins. It plays a critical role in the recognition and repair of mismatched bases that occur during DNA replication particularly in the G2 phase of the cell cycle. Through its involvement in the mismatch repair pathway MLH1 is connected to cell cycle regulation and the DNA damage response pathway.

Associated diseases and disorders

MLH1 mutations are closely linked to Lynch syndrome and sporadic colorectal cancer. Lynch syndrome a hereditary condition significantly raises the risk of colorectal cancer and other cancers due to defective DNA mismatch repair. MLH1 mutations often lead to the loss of MLH1 protein expression particularly observed in MLH1 IHC staining. Additionally in colorectal cancer the MLH1 protein may interact with APC and TP53 playing a role in cancer progression and tumorigenesis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-MLH1 antibody [EPR3893] (ab108622), expandable thumbnail

    Western blot - Anti-MLH1 antibody [EPR3893] (ab108622)

    Lanes 1-4: Merged signal (red and green). Green - ab108622 observed at 90 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.

    ab108622 Anti-MLH1 antibody [EPR3893] was shown to specifically react with MLH1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human MLH1 knockout HeLa cell line ab267223 (knockout cell lysate Human MLH1 knockout HeLa cell lysate ab257172) was used. Wild-type and MLH1 knockout samples were subjected to SDS-PAGE. ab108622 and Anti-GAPDH antibody [6C5] - Loading Control were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-MLH1 antibody [EPR3893] (ab108622) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: MLH1 knockout HeLa cell lysate at 20 µg

    Lane 3: Jurkat cell lysate at 20 µg

    Lane 4: HCT116 cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 85 kDa

    Observed band size: 85 kDa

  • Western blot - Anti-MLH1 antibody [EPR3893] (ab108622), expandable thumbnail

    Western blot - Anti-MLH1 antibody [EPR3893] (ab108622)

    Lanes 1 - 4: Merged signal (red and green). Green - ab108622 observed at 88 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.


    ab108622 was shown to recognize MLH1 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when MLH1 knockout samples were examined. Wild-type and MLH1 knockout samples were subjected to SDS-PAGE. ab108622 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-MLH1 antibody [EPR3893] (ab108622) at 1/1000 dilution

    Lane 1: Wild-type HAP1 cell lysate at 20 µg

    Lane 2: MLH1 knockout HAP1 cell lysate at 20 µg

    Lane 3: HCT116 cell lysate at 20 µg

    Lane 4: 293T cell lysate at 20 µg

    Predicted band size: 85 kDa

  • Western blot - Anti-MLH1 antibody [EPR3893] (ab108622), expandable thumbnail

    Western blot - Anti-MLH1 antibody [EPR3893] (ab108622)

    All lanes: Western blot - Anti-MLH1 antibody [EPR3893] (ab108622) at 1/1000 dilution

    Lane 1: 293 cell lysate at 10 µg

    Lane 2: Jurkat cell lysate at 10 µg

    Lane 3: K562 cell lysate at 10 µg

    Lane 4: SH-SY5Y cell lysate at 10 µg

    Predicted band size: 85 kDa

  • Western blot - Anti-MLH1 antibody [EPR3893] (ab108622), expandable thumbnail
    This image is courtesy of an anonymous customer review.

    Western blot - Anti-MLH1 antibody [EPR3893] (ab108622)

    All lanes: Western blot - Anti-MLH1 antibody [EPR3893] (ab108622) at 1/1000 dilution

    Lane 1: Hela Cells Whole Cell Lysates at 30 µg

    Lane 2: HCT116 Whole Cell Lysates at 30 µg

    Secondary

    All lanes: Goat Polyclonal to Rabbit IgG (HRP) at 1/2000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 85 kDa

    Exposure time: 30s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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