Anti-MLH1 antibody [EPR3893]

• WB

Supplier Data

Western blot - Anti-MLH1 antibody [EPR3893] (AB108622)

Lanes 1-4 : Merged signal (red and green). Green - ab108622 observed at 90 kDa. Red - loading control ab8245 observed at 37 kDa.

ab108622 Anti-MLH1 antibody [EPR3893] was shown to specifically react with MLH1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab267223 (knockout cell lysate ab257172) was used. Wild-type and MLH1 knockout samples were subjected to SDS-PAGE. ab108622 and Anti-GAPDH antibody [6C5] - Loading Control were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-MLH1 antibody [EPR3893] (ab108622) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

MLH1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human MLH1 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-mlh1-knockout-hela-cell-line-ab267223'>ab267223</a>)

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

HCT116 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 85 kDa

Observed band size: 85 kDa

false

• WB

Lab

Western blot - Anti-MLH1 antibody [EPR3893] (AB108622)

Lanes 1 - 4 : Merged signal (red and green). Green - ab108622 observed at 88 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab108622 was shown to recognize MLH1 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when MLH1 knockout samples were examined. Wild-type and MLH1 knockout samples were subjected to SDS-PAGE. ab108622 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-MLH1 antibody [EPR3893] (ab108622) at 1/1000 dilution

Lane 1:

Wild-type HAP1 cell lysate at 20 µg

Lane 2:

MLH1 knockout HAP1 cell lysate at 20 µg

Lane 3:

HCT116 cell lysate at 20 µg

Lane 4:

293T cell lysate at 20 µg

Predicted band size: 85 kDa

false

• WB

AbReview49658****

Western blot - Anti-MLH1 antibody [EPR3893] (AB108622)

All lanes:

Western blot - Anti-MLH1 antibody [EPR3893] (ab108622) at 1/1000 dilution

Lane 1:

Hela Cells Whole Cell Lysates at 30 µg

Lane 2:

HCT116 Whole Cell Lysates at 30 µg

Secondary

All lanes:

Goat Polyclonal to Rabbit IgG (HRP) at 1/2000 dilution

Predicted band size: 85 kDa

true

Exposure time: 30s

This image is courtesy of an anonymous abreview.

• WB

Unknown

Western blot - Anti-MLH1 antibody [EPR3893] (AB108622)

All lanes:

Western blot - Anti-MLH1 antibody [EPR3893] (ab108622) at 1/1000 dilution

Lane 1:

293 cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Lane 4:

SH-SY5Y cell lysate at 10 µg

Predicted band size: 85 kDa

false