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AB111942

Anti-MLH3 antibody

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(1 Publication)

Rabbit Polyclonal MLH3 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MLH3 aa 1 to C-terminus.

View Alternative Names

DNA mismatch repair protein Mlh3, MutL protein homolog 3, MLH3

1 Images
Western blot - Anti-MLH3 antibody (AB111942)
  • WB

Unknown

Western blot - Anti-MLH3 antibody (AB111942)

5% SDS PAGE

All lanes:

Western blot - Anti-MLH3 antibody (ab111942) at 1/1000 dilution

All lanes:

HCT116 whole cell lysate at 30 µg

Predicted band size: 142 kDa,164 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human MLH3 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UHC1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab111942 is purified by antigen-affinity chromatography.
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MLH3 also known as MutL homolog 3 is a DNA mismatch repair protein part of the MutL family. It has a molecular mass of approximately 160 kilodaltons (kDa). This protein is mainly expressed in the testis and also found in various other tissues at lower levels. MLH3 plays a mechanical role in DNA repair through the correction of mismatches that occur during replication.
Biological function summary

MLH3 acts within DNA mismatch repair processes to maintain genomic stability. It forms heterodimeric complexes with MLH1 another MutL homolog which is essential for its DNA repair function. This complex carries out the recognition and repair of insertion-deletion loops and base-base mismatches in DNA therefore ensuring fidelity during cell division.

Pathways

MLH3 operates within the DNA damage response and repair pathways. It interacts significantly with the MLH1 protein to facilitate mismatch repair activities. Additionally MLH3 participates in the homologous recombination pathway working alongside proteins such as MSH2 to secure proper chromosome segregation during meiosis.

MLH3 mutations or dysfunctions have associations with Lynch syndrome a hereditary condition that increases cancer risk especially colorectal cancer. Alterations in MLH3 in combination with MLH1 mutations have implications for mismatch repair deficiencies observed in these disorders. Additionally variances in MLH3 contribute to the progression of endometrial cancer often linked with other mismatch repair proteins like MSH6 in the disease pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probably involved in the repair of mismatches in DNA.
See full target information MLH3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 8:4670 PubMed29549309

2018

Coupling of SK channels, L-type Ca channels, and ryanodine receptors in cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Dong Zhang,Zana A Coulibaly,Wei Chun Chen,Hannah A Ledford,Jeong Han Lee,Padmini Sirish,Gu Dai,Zhong Jian,Frank Chuang,Ingrid Brust-Mascher,Ebenezer N Yamoah,Ye Chen-Izu,Leighton T Izu,Nipavan Chiamvimonvat
View all publications
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Product promise

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