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AB221066

Anti-MLH3 antibody

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(1 Publication)

Rabbit Polyclonal MLH3 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MLH3 aa 1300-1450.

View Alternative Names

DNA mismatch repair protein Mlh3, MutL protein homolog 3, MLH3

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-MLH3 antibody (AB221066)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MLH3 antibody (AB221066)

Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized U-2 OS cells labeling MLH3 with ab221066 at 4 μg/mL (green).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human MLH3 aa 1300-1450. The exact immunogen used to generate this antibody is proprietary information.

Q9UHC1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MLH3 also known as MutL homolog 3 is a DNA mismatch repair protein part of the MutL family. It has a molecular mass of approximately 160 kilodaltons (kDa). This protein is mainly expressed in the testis and also found in various other tissues at lower levels. MLH3 plays a mechanical role in DNA repair through the correction of mismatches that occur during replication.
Biological function summary

MLH3 acts within DNA mismatch repair processes to maintain genomic stability. It forms heterodimeric complexes with MLH1 another MutL homolog which is essential for its DNA repair function. This complex carries out the recognition and repair of insertion-deletion loops and base-base mismatches in DNA therefore ensuring fidelity during cell division.

Pathways

MLH3 operates within the DNA damage response and repair pathways. It interacts significantly with the MLH1 protein to facilitate mismatch repair activities. Additionally MLH3 participates in the homologous recombination pathway working alongside proteins such as MSH2 to secure proper chromosome segregation during meiosis.

MLH3 mutations or dysfunctions have associations with Lynch syndrome a hereditary condition that increases cancer risk especially colorectal cancer. Alterations in MLH3 in combination with MLH1 mutations have implications for mismatch repair deficiencies observed in these disorders. Additionally variances in MLH3 contribute to the progression of endometrial cancer often linked with other mismatch repair proteins like MSH6 in the disease pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probably involved in the repair of mismatches in DNA.
See full target information MLH3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell biology and toxicology 39:2069-2087 PubMed35142956

2022

Immortalization-upregulated protein promotes pancreatic cancer progression by regulating NPM1/FHL1-mediated cell-cycle-checkpoint protein activity.

Applications

Unspecified application

Species

Unspecified reactive species

Qiankun Luo,Yanfeng Pan,Qiang Fu,Xu Zhang,Shuai Zhou,Pengfei Yu,Huiyuan Tian,Pan Liu,Song Chen,Hongwei Zhang,Tao Qin
View all publications
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