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Rabbit Recombinant Monoclonal MLK3 antibody. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 16 publications.

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Images

Western blot - Anti-MLK3 antibody [EP1460Y] (AB51068), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MLK3 antibody [EP1460Y] (AB51068), expandable thumbnail
  • Western blot - Anti-MLK3 antibody [EP1460Y] (AB51068), expandable thumbnail
  • Western blot - Anti-MLK3 antibody [EP1460Y] (AB51068), expandable thumbnail
  • Western blot - Anti-MLK3 antibody [EP1460Y] (AB51068), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.01% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFFlow CytWB
Human
Tested
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

For unpurified use at 1/5000.

Associated Products

Select an associated product type

2 products for Alternative Product

1 product for Alternative Version

Target data

Function

Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1) through phosphorylation and activation of MAP2K4/MKK4 and MAP2K7/MKK7. Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.

Alternative names

MLK3, PTK1, SPRK, MAP3K11, Mitogen-activated protein kinase kinase kinase 11, Mixed lineage kinase 3, Src-homology 3 domain-containing proline-rich kinase

Recommended products

Rabbit Recombinant Monoclonal MLK3 antibody. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 16 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP1460Y
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

MLK3 also known as MEKK11 is a kinase widely expressed in human tissues. This protein with a molecular mass of approximately 97 kDa functions as a mitogen-activated protein kinase kinase kinase (MAP3K). MLK3 mechanically activates the c-Jun N-terminal kinase (JNK) and p38 MAP kinase pathways by phosphorylating downstream MAP2Ks. It is involved in various cell processes including apoptosis and inflammation.

Biological function summary

MLK3 plays a critical role in cellular response to stress and inflammation. It acts as part of a signaling complex that regulates the JNK and p38 pathways key mediators in immune responses. These pathways control the production of pro-inflammatory cytokines and the cellular stress response. Through its kinase activity MLK3 supports the activation of transcription factors such as AP-1 which drive gene expression changes needed for its biological functions.

Pathways

MLK3 is intricately involved in the MAPK signaling cascade. It connects with other MAP3Ks like MLK1 and MLK2 contributing to the complexity of MAPK signaling. MLK3’s participation in the JNK and p38 pathways links it to cytokine signaling and cellular stress responses. These pathways are significant for maintaining cellular homeostasis and coordinating responses to external stimuli.

Associated diseases and disorders

MLK3 has implications in cancer and neurodegenerative diseases. In cancer MLK3 modulates pathways that influence cell proliferation and survival potentially leading to tumor growth and metastasis when dysregulated. Similarly altered MLK3 signaling is observed in neurodegenerative diseases possibly due to its role in managing cellular stress. MLK3 interacts with proteins like JNK and p38 which are important in disease mechanisms and progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-MLK3 antibody [EP1460Y] (ab51068), expandable thumbnail

    Western blot - Anti-MLK3 antibody [EP1460Y] (ab51068)

    Blocking buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-MLK3 antibody [EP1460Y] (ab51068) at 1/1000 dilution

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

    Lane 2: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 15 µg

    Lane 3: A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 93 kDa

    Observed band size: 100 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-MLK3 antibody [EP1460Y] (ab51068), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MLK3 antibody [EP1460Y] (ab51068)

    Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling MLK3 with Purified ab51068 at 1:50 dilution (3.52 μg/ml). Cells were fixed in 100% Methanol and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488,Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-MLK3 antibody [EP1460Y] (ab51068), expandable thumbnail

    Western blot - Anti-MLK3 antibody [EP1460Y] (ab51068)

    Lanes 1- 2: Merged signal (red and green). Green - ab51068 observed at 105 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.

    ab51068 was shown to react with MLK3 in wild-type A549 cells in western blot. Loss of signal was observed when knockout cell line Human MAP3K11 (MLK3) knockout A549 cell line ab267169 (knockout cell lysate Human MAP3K11 (MLK3) knockout A549 cell lysate ab257519) was used. Wild-type A549 and MAP3K11 knockout A549 cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab51068 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-MLK3 antibody [EP1460Y] (ab51068) at 1/5000 dilution

    Lane 1: Wild-type A549 cell lysate at 20 µg

    Lane 2: MAP3K11 knockout A549 cell lysate at 20 µg

    Lane 2: Western blot - Human MAP3K11 (MLK3) knockout A549 cell line (Human MAP3K11 (MLK3) knockout A549 cell line ab267169)

    Performed under reducing conditions.

    Predicted band size: 93 kDa

    Observed band size: 105 kDa

  • Western blot - Anti-MLK3 antibody [EP1460Y] (ab51068), expandable thumbnail

    Western blot - Anti-MLK3 antibody [EP1460Y] (ab51068)

    Lanes 1- 2: Merged signal (red and green). Green - ab51068 observed at 105 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.

    ab51068 was shown to react with MLK3 in wild-type A549 cells in western blot. Loss of signal was observed when knockout cell line Human MAP3K11 (MLK3) knockout A549 cell line ab267168 (knockout cell lysate Human MAP3K11 (MLK3) knockout A549 cell lysate ab257518) was used. Wild-type A549 and MAP3K11 knockout A549 cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab51068 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-MLK3 antibody [EP1460Y] (ab51068) at 1/5000 dilution

    Lane 1: Wild-type A549 cell lysate at 20 µg

    Lane 2: MAP3K11 knockout A549 cell lysate at 20 µg

    Lane 2: Western blot - Human MAP3K11 (MLK3) knockout A549 cell line (Human MAP3K11 (MLK3) knockout A549 cell line ab267168)

    Performed under reducing conditions.

    Predicted band size: 93 kDa

    Observed band size: 105 kDa

  • Western blot - Anti-MLK3 antibody [EP1460Y] (ab51068), expandable thumbnail

    Western blot - Anti-MLK3 antibody [EP1460Y] (ab51068)

    Lane 1: Wild-type HAP1 cell lysate (20 μg)
    Lane 2: MLK3 knockout HAP1 cell lysate (20 μg)
    Lane 3: A431 cell lysate (20 μg)
    Lane 4: HeLa cell lysate (20 μg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab51068 observed at 105 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    ab51068 was shown to specifically react with MLK3 when MLK3 knockout samples were used. Wild-type and MLK3 knockout samples were subjected to SDS-PAGE. ab51068 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/5000 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-MLK3 antibody [EP1460Y] (ab51068)

    Predicted band size: 93 kDa

    Observed band size: 105 kDa

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Product protocols

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