AB191530

Anti-MLK3 (phospho T277 + S281) antibody

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(10 Publications )

Rabbit Polyclonal MLK3 phospho S281 + T277 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human MAP3K11 pS281 + T277.

1 Images

Western blot - Anti-MLK3 (phospho T277 + S281) antibody (AB191530)

WB

Supplier Data

Western blot - Anti-MLK3 (phospho T277 + S281) antibody (AB191530)

All lanes:

Western blot - Anti-MLK3 (phospho T277 + S281) antibody (ab191530)

Lane 1:

HEK293T whole cell lysate

Lane 2:

NIH 3T3 whole cell lysate

Lane 3:

PC12 whole cell lysate

Predicted band size: 93 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

Immunogen

Synthetic Peptide within Human MAP3K11 pS281 + T277. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab191530 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage buffer

pH: 7.2 Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MLK3 also known as MEKK11 is a kinase widely expressed in human tissues. This protein with a molecular mass of approximately 97 kDa functions as a mitogen-activated protein kinase kinase kinase (MAP3K). MLK3 mechanically activates the c-Jun N-terminal kinase (JNK) and p38 MAP kinase pathways by phosphorylating downstream MAP2Ks. It is involved in various cell processes including apoptosis and inflammation.

Biological function summary

MLK3 plays a critical role in cellular response to stress and inflammation. It acts as part of a signaling complex that regulates the JNK and p38 pathways key mediators in immune responses. These pathways control the production of pro-inflammatory cytokines and the cellular stress response. Through its kinase activity MLK3 supports the activation of transcription factors such as AP-1 which drive gene expression changes needed for its biological functions.

Pathways

MLK3 is intricately involved in the MAPK signaling cascade. It connects with other MAP3Ks like MLK1 and MLK2 contributing to the complexity of MAPK signaling. MLK3's participation in the JNK and p38 pathways links it to cytokine signaling and cellular stress responses. These pathways are significant for maintaining cellular homeostasis and coordinating responses to external stimuli.

MLK3 has implications in cancer and neurodegenerative diseases. In cancer MLK3 modulates pathways that influence cell proliferation and survival potentially leading to tumor growth and metastasis when dysregulated. Similarly altered MLK3 signaling is observed in neurodegenerative diseases possibly due to its role in managing cellular stress. MLK3 interacts with proteins like JNK and p38 which are important in disease mechanisms and progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1) through phosphorylation and activation of MAP2K4/MKK4 and MAP2K7/MKK7. Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.

See full target information MAP3K11 pS281 + T277

Alternative Names

MLK3, PTK1, SPRK, MAP3K11, Mitogen-activated protein kinase kinase kinase 11, Mixed lineage kinase 3, Src-homology 3 domain-containing proline-rich kinase

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Publications (10)

Recent publications for all applications. Explore the full listand refine your search

Nature communications 14:4101 PubMed37491347

2023

Macrophage-to-endothelial cell crosstalk by the cholesterol metabolite 27HC promotes atherosclerosis in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Liming Yu,Lin Xu,Haiyan Chu,Jun Peng,Anastasia Sacharidou,Hsi-Hsien Hsieh,Ada Weinstock,Sohaib Khan,Liqian Ma,José Gabriel Barcia Durán,Jeffrey McDonald,Erik R Nelson,Sunghee Park,Donald P McDonnell,Kathryn J Moore,Lily Jun-Shen Huang,Edward A Fisher,Chieko Mineo,Linzhang Huang,Philip W Shaul

Nature communications 13:2982 PubMed35624087

2022

PI(18:1/18:1) is a SCD1-derived lipokine that limits stress signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Thürmer,André Gollowitzer,Helmut Pein,Konstantin Neukirch,Elif Gelmez,Lorenz Waltl,Natalie Wielsch,René Winkler,Konstantin Löser,Julia Grander,Madlen Hotze,Sönke Harder,Annika Döding,Martina Meßner,Fabiana Troisi,Maximilian Ardelt,Hartmut Schlüter,Johanna Pachmayr,Óscar Gutiérrez-Gutiérrez,Karl Lenhard Rudolph,Kathrin Thedieck,Ulrike Schulze-Späte,Cristina González-Estévez,Christian Kosan,Aleš Svatoš,Marcel Kwiatkowski,Andreas Koeberle

JCI insight 6: PubMed34324442

2021

MLK3 mediates impact of PKG1α on cardiac function and controls blood pressure through separate mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Timothy D Calamaras,Suchita Pande,Robert Au Baumgartner,Seung Kyum Kim,Joseph C McCarthy,Gregory L Martin,Kelly Tam,Angela L McLaughlin,Guang-Rong Wang,Mark J Aronovitz,Weiyu Lin,Jonathan I Aguirre,Paulina Baca,Peiwen Liu,Daniel A Richards,Roger J Davis,Richard H Karas,Iris Z Jaffe,Robert M Blanton

Cell death & disease 11:574 PubMed32710001

2020

Pyroptosis and ferroptosis induced by mixed lineage kinase 3 (MLK3) signaling in cardiomyocytes are essential for myocardial fibrosis in response to pressure overload.

Applications

Unspecified application

Species

Unspecified reactive species

Junyan Wang,Bo Deng,Qing Liu,Yusheng Huang,Weitao Chen,Jing Li,Zheng Zhou,Lu Zhang,Birong Liang,Jiaqi He,Zixin Chen,Cui Yan,Zhongqi Yang,Shaoxiang Xian,Lingjun Wang

Journal of Zhejiang University. Science. B 21:234-245 PubMed32133800

2020

Amyloid precursor protein regulates 5-fluorouracil resistance in human hepatocellular carcinoma cells by inhibiting the mitochondrial apoptotic pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Long Wu,Ying Chen,Wen-Cui Kong,Zhong-Quan Zhao

International journal of molecular medicine 45:162-174 PubMed31746365

2019

Amyloid precursor protein promotes the migration and invasion of breast cancer cells by regulating the MAPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiong Wu,Shuanglong Chen,Chuanhui Lu

Journal of virology 93: PubMed31270223

2019

High-Throughput Screening Identifies Mixed-Lineage Kinase 3 as a Key Host Regulatory Factor in Zika Virus Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Xu,Min Cheng,Xiaojing Chi,Xiuying Liu,Jia Zhou,Tianli Lin,Wei Yang

International journal of molecular medicine 43:2086-2102 PubMed30864680

2019

Protective effects of CXCR3/HO‑1 gene‑modified BMMSCs on damaged intestinal epithelial cells: Role of the p38‑MAPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Mingli Yin,Zhongyang Shen,Liu Yang,Weiping Zheng,Hongli Song

Science signaling 11: PubMed30459284

2018

IFN-γ-inducible antiviral responses require ULK1-mediated activation of MLK3 and ERK5.

Applications

Unspecified application

Species

Unspecified reactive species

Diana Saleiro,Gavin T Blyth,Ewa M Kosciuczuk,Patrick A Ozark,Beata Majchrzak-Kita,Ahmet D Arslan,Mariafausta Fischietti,Neha K Reddy,Curt M Horvath,Roger J Davis,Eleanor N Fish,Leonidas C Platanias

Journal of cellular biochemistry 118:3249-3259 PubMed28262979

2017

Mixed Lineage Kinase 3 Mediates the Induction of CXCL10 by a STAT1-Dependent Mechanism During Hepatocyte Lipotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Kyoko Tomita,Ayano Kabashima,Brittany L Freeman,Steven F Bronk,Petra Hirsova,Samar H Ibrahim

View all publications

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