AB93798

Anti-MLK4 antibody

Name: Anti-MLK4 antibody (ab93798) | Abcam
Brand: Abcam Limited
SKU: AB93798

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(2 Publications)

Rabbit Polyclonal MLK4 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human MAP3K21 aa 950 to C-terminus.

View Alternative Names

KIAA1804, MLK4, MAP3K21, Mitogen-activated protein kinase kinase kinase 21, Mitogen-activated protein kinase kinase kinase MLK4, Mixed lineage kinase 4

2 Images

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Immunoprecipitation - Anti-MLK4 antibody (AB93798)

Detection of MLK4 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab93798 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection : Chemiluminescence with an exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-MLK4 antibody (ab93798)

Predicted band size: 114 kDa

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Unknown

Western blot - Anti-MLK4 antibody (AB93798)

All lanes:

Western blot - Anti-MLK4 antibody (ab93798) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 114 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human MAP3K21 aa 950 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q5TCX8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MLK4 also known as Mixed Lineage Kinase 4 is a signaling protein involved in various cellular processes. It is a member of the MAP3K family and has a molecular weight of approximately 88 kDa. MLK4 is expressed in several tissues including the brain liver and kidney suggesting it plays roles in diverse physiological contexts. As a kinase it participates in phosphorylating specific substrates which impacts downstream signaling pathways.

Biological function summary

MLK4 influences cell proliferation differentiation and apoptosis through its kinase activity. It is part of signaling complexes that coordinate cellular responses to external stimuli linking surface receptors to the intracellular machinery. Through these roles MLK4 exerts important effects on cellular dynamics and maintenance of normal physiological balance.

Pathways

MLK4 integrates into the JNK and p38 MAPK signaling cascades which mediate responses to stress stimuli and regulate gene expression. MLK4 interacts with various other proteins including MLK3 to modulate these pathways effectively. Furthermore its role in these pathways positions MLK4 as an important player in ensuring cells react appropriately to potential damage or stress signals.

MLK4 has been associated with certain cancers and neurodegenerative diseases. Its involvement in cancer relates to its ability to influence cell growth and survival where MLK4 mutations or dysregulation might promote tumorigenesis. Moreover altered MLK4 signaling has links to Parkinson's disease particularly in conjunction with other kinases like LRRK2 suggesting its potential as a therapeutic target for modulating disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Negative regulator of TLR4 signaling. Does not activate JNK1/MAPK8 pathway, p38/MAPK14, nor ERK2/MAPK1 pathways.

See full target information MAP3K21

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Oncogenesis 12:35 PubMed37407566

2023

MLK4 promotes glucose metabolism in lung adenocarcinoma through CREB-mediated activation of phosphoenolpyruvate carboxykinase and is regulated by KLF5.

Applications

Unspecified application

Species

Unspecified reactive species

Alvin Ho-Kwan Cheung,Kit-Yee Wong,Xiaoli Liu,Fenfen Ji,Chris Ho-Lam Hui,Yihan Zhang,Johnny Sheung-Him Kwan,Bonan Chen,Yujuan Dong,Raymond Wai-Ming Lung,Jun Yu,Kwok Wai Lo,Chi Chun Wong,Wei Kang,Ka-Fai To

Experimental and therapeutic medicine 16:3317-3324 PubMed30233678

2018

Mixed lineage kinase-4 promotes gastric carcinoma tumorigenesis through suppression of the c-Jun N-terminal kinase signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Xi,Jianhua Niu,Dongmei Li,Jiagen He,Le Qin,Xinyu Peng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MLK4 antibody

Immunoprecipitation - Anti-MLK4 antibody (AB93798)

Western blot - Anti-MLK4 antibody (AB93798)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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