AB263021

Anti-MLLT6 antibody [EPR20977-52] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal MLLT6 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse samples.

View Alternative Names

AF17, MLLT6, Protein AF-17, ALL1-fused gene from chromosome 17 protein

1 Images

Lab

Western blot - Anti-MLLT6 antibody [EPR20977-52] - BSA and Azide free (AB263021)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody detects an unidentified band above the target band.

Exposure time : 26 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208017).

All lanes:

Western blot - Anti-MLLT6 antibody [EPR20977-52] (<a href='/en-us/products/primary-antibodies/mllt6-antibody-epr20977-52-ab208017'>ab208017</a>) at 1/1000 dilution

Lanes 1 - 2:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 3:

K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 20 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/25000 dilution

Predicted band size: 112 kDa

Observed band size: 112 kDa

false

Unconjugated

Anti-MLLT6 antibody [EPR20977-52]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20977-52

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab263021 is the carrier-free version of ab208017.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MLLT6 protein also known as AF17 functions mechanically as a transcriptional coactivator. It plays a role in regulating gene expression by interacting with other proteins involved in transcriptional control. The protein has a mass of approximately 122 kDa. Researchers identify its expression in various tissues with a significant presence in hematopoietic tissues. MLLT6 interacts closely with other protein partners to modulate the transcriptional machinery.

Biological function summary

MLLT6 acts as a part of larger protein complexes involved in transcription regulation. It influences the activation and repression of specific gene sets necessary for cell differentiation and development. By integrating into these complexes MLLT6 assists in maintaining the proper function of gene expression programs that control cellular identity and activity. Its contribution is important in processes such as hematopoiesis impacting the development and specialization of blood cells.

Pathways

MLLT6 plays a role in key regulatory networks like the mitochondrial apoptosis and Notch signaling pathways. Through interactions within these pathways MLLT6 associates with proteins like MLLT3 and NOTCH1. These relationships help mediate cell survival proliferation and differentiation signals. This pathway integration allows MLLT6 to influence essential cellular processes aligning transcriptional responses with extracellular signals.

MLLT6 shows a strong connection to certain types of leukemia notably acute myeloid leukemia (AML). Dysregulation of MLLT6 can contribute to leukemogenesis by disrupting normal transcriptional programs and signaling pathways. Additionally studies link MLLT6 to neuroblastoma where its aberrant expression intersects with the MLL protein family impacting tumor development. These disease associations highlight MLLT6's potential value as a therapeutic target and a biomarker for specific hematological and neurological malignancies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information MLLT6

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MLLT6 antibody [EPR20977-52] - BSA and Azide free

Western blot - Anti-MLLT6 antibody [EPR20977-52] - BSA and Azide free (AB263021)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Product promise