Rabbit Recombinant Monoclonal MLLT6 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse samples.
Images
![Western blot - Anti-MLLT6 antibody [EPR20977-52] - BSA and Azide free (AB263021), expandable thumbnail](https://content.abcam.com/products/images/mllt6-antibody-epr20977-52-bsa-and-azide-free-ab263021--western-blot-img56746.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MLLT6 antibody [EPR20977-52] - BSA and Azide free (AB263021)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Predicted | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Target data
Alternative names
AF17, MLLT6, Protein AF-17, ALL1-fused gene from chromosome 17 protein
Recommended products
Rabbit Recombinant Monoclonal MLLT6 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR20977-52
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab263021 is the carrier-free version of Anti-MLLT6 antibody [EPR20977-52] ab208017.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The MLLT6 protein also known as AF17 functions mechanically as a transcriptional coactivator. It plays a role in regulating gene expression by interacting with other proteins involved in transcriptional control. The protein has a mass of approximately 122 kDa. Researchers identify its expression in various tissues with a significant presence in hematopoietic tissues. MLLT6 interacts closely with other protein partners to modulate the transcriptional machinery.
- Biological function summary
MLLT6 acts as a part of larger protein complexes involved in transcription regulation. It influences the activation and repression of specific gene sets necessary for cell differentiation and development. By integrating into these complexes MLLT6 assists in maintaining the proper function of gene expression programs that control cellular identity and activity. Its contribution is important in processes such as hematopoiesis impacting the development and specialization of blood cells.
- Pathways
MLLT6 plays a role in key regulatory networks like the mitochondrial apoptosis and Notch signaling pathways. Through interactions within these pathways MLLT6 associates with proteins like MLLT3 and NOTCH1. These relationships help mediate cell survival proliferation and differentiation signals. This pathway integration allows MLLT6 to influence essential cellular processes aligning transcriptional responses with extracellular signals.
- Associated diseases and disorders
MLLT6 shows a strong connection to certain types of leukemia notably acute myeloid leukemia (AML). Dysregulation of MLLT6 can contribute to leukemogenesis by disrupting normal transcriptional programs and signaling pathways. Additionally studies link MLLT6 to neuroblastoma where its aberrant expression intersects with the MLL protein family impacting tumor development. These disease associations highlight MLLT6's potential value as a therapeutic target and a biomarker for specific hematological and neurological malignancies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-MLLT6 antibody [EPR20977-52] - BSA and Azide free (ab263021), expandable thumbnail](https://content.abcam.com/products/images/mllt6-antibody-epr20977-52-bsa-and-azide-free-ab263021--western-blot-img56746.jpg "Western blot - Anti-MLLT6 antibody [EPR20977-52] - BSA and Azide free (ab263021)")
Western blot - Anti-MLLT6 antibody [EPR20977-52] - BSA and Azide free (ab263021)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This antibody detects an unidentified band above the target band.
Exposure time: 26 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MLLT6 antibody [EPR20977-52] ab208017).
All lanes: Western blot - Anti-MLLT6 antibody [EPR20977-52] (Anti-MLLT6 antibody [EPR20977-52] ab208017) at 1/1000 dilution
Lanes 1 - 2: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 3: K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 20 µg
Lane 4: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/25000 dilution
Predicted band size: 112 kDa
Observed band size: 112 kDa
Downloads
Product protocols
- Visit the general protocols
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