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AB3478

Anti-MLN64 antibody

5

(1 Review)

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(20 Publications)

Rabbit Polyclonal MLN64 antibody. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Cited in 20 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human STARD3.

View Alternative Names

CAB1, MLN64, STARD3, StAR-related lipid transfer protein 3, Metastatic lymph node gene 64 protein, Protein CAB1, START domain-containing protein 3, MLN 64, StARD3

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-MLN64 antibody (AB3478)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MLN64 antibody (AB3478)

ICC/IF image of ab3478 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3478, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Western blot - Anti-MLN64 antibody (AB3478)
  • WB

Unknown

Western blot - Anti-MLN64 antibody (AB3478)

Western blot of MLN64 on rat adrenal gland extract using ab3478.

All lanes:

Western blot - Anti-MLN64 antibody (ab3478) at 1 µg/mL

All lanes:

Lysates prepared from rat adrenal gland

Predicted band size: 51 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human STARD3.

Q14849

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p>By Western blot, this antibody detects an ~53 kDa protein representing MLN 64 from rat adrenal gland tissue extracts. This antibody also detects an ~25 kDa protein from rat adrenal gland which may correspond to degradation product.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MLN64 also known as StAR-related lipid transfer domain protein 3 (STARD3) plays an important role in intracellular cholesterol transport. It has a molecular weight of approximately 50 kDa. MLN64 is mainly expressed in the adrenal gland placenta and various cancerous tissues. As a transmembrane protein located in the endoplasmic reticulum and late endosomes it facilitates lipid transport between membranes by binding cholesterol through its START domain.
Biological function summary

MLN64 influences cholesterol homeostasis by transporting cholesterol from late endosomes to the mitochondria. It acts outside the context of a known protein complex yet exerts significant control over cholesterol regulation within cells. By controlling cholesterol distribution MLN64 supports membrane synthesis steroid production and cellular signaling processes. Its function is integral to maintaining lipid balance within different cell types.

Pathways

MLN64 is essential for lipid metabolism and steroid biosynthesis. It directly links with the cholesterol trafficking pathway where it interacts with proteins such as Niemann-Pick C1 (NPC1). This interplay facilitates cholesterol efflux from late endosomes a critical step in cellular lipid regulation. Additionally MLN64's activity affects the steroidogenic pathway aiding in the conversion of cholesterol into steroidal hormones through cooperation with hormone-sensitive lipase.

MLN64 has connections to conditions such as Niemann-Pick disease and steroidal imbalances. Dysfunctional cholesterol transport often involving NPC1 can manifest in Niemann-Pick disease illustrating MLN64's important involvement. Also altered MLN64 activity affects steroid hormone levels contributing to disorders related to abnormal steroid synthesis. Understanding MLN64's multifaceted role in these conditions may offer insights for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Sterol-binding protein that mediates cholesterol transport from the endoplasmic reticulum to endosomes (PubMed : 11053434, PubMed : 15930133, PubMed : 22514632, PubMed : 28377464, PubMed : 33124732). The sterol transport mechanism is triggered by phosphorylation of FFAT motif that leads to membrane tethering between the endoplasmic reticulum and late endosomes via interaction with VAPA and VAPB (PubMed : 24105263, PubMed : 28377464, PubMed : 33124732). Acts as a lipid transfer protein that redirects sterol to the endosome at the expense of the cell membrane and favors membrane formation inside endosomes (PubMed : 28377464). May also mediate cholesterol transport between other membranes, such as mitochondria membrane or cell membrane (PubMed : 12070139, PubMed : 19965586). However, such results need additional experimental evidences; probably mainly mediates cholesterol transport from the endoplasmic reticulum to endosomes (PubMed : 28377464). Does not activate transcriptional cholesterol sensing (PubMed : 28377464). Able to bind other lipids, such as lutein, a xanthophyll carotenoids that form the macular pigment of the retina (PubMed : 21322544).
See full target information STARD3
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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

Cancer communications (London, England) 44:47-75 PubMed38133457

2023

Glucose-mediated mitochondrial reprogramming by cholesterol export at TM4SF5-enriched mitochondria-lysosome contact sites.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Eon Kim,So-Young Park,Chulhwan Kwak,Yoonji Lee,Dae-Geun Song,Jae Woo Jung,Haesong Lee,Eun-Ae Shin,Yangie Pinanga,Kyung-Hee Pyo,Eun Hae Lee,Wonsik Kim,Soyeon Kim,Chang-Duck Jun,Jeanho Yun,Sun Choi,Hyun-Woo Rhee,Kwang-Hyeon Liu,Jung Weon Lee

American journal of cancer research 13:5151-5173 PubMed38058811

2023

StAR-related lipid transfer domain protein 3 (STARD3) regulates HER2 and promotes HER2-positive breast cancer progression through interaction with HSP90 and SRC signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Doan Huu Nhat Binh,Tzu-Chun Cheng,Shih-Hsin Tu,You-Cheng Liao,Yi-Yuan Yang,Li-Ching Chen,Yuan-Soon Ho

Polish journal of pathology : official journal of the Polish Society of Pathologists 73:215-222 PubMed36734436

2023

The significance of StAR-related lipid transfer protein-3 expression in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Abdulfattah Fararjeh,Ezidin Kaddumi,Ali Al-Khader,Walid Aburayyan

Frontiers in cell and developmental biology 10:743287 PubMed35309917

2022

Negative Modulation of Macroautophagy by Stabilized HERPUD1 is Counteracted by an Increased ER-Lysosomal Network With Impact in Drug-Induced Stress Cell Survival.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriela Vargas,Omar Cortés,Eloisa Arias-Muñoz,Sergio Hernández,Cristobal Cerda-Troncoso,Laura Hernández,Alexis E González,Michael H Tatham,Hianara A Bustamante,Claudio Retamal,Jorge Cancino,Manuel Varas-Godoy,Ronald T Hay,Alejandro Rojas-Fernández,Viviana A Cavieres,Patricia V Burgos

Biochimica et biophysica acta. Molecular and cell biology of lipids 1866:158927 PubMed33771709

2021

Overexpression of STARD3 attenuates oxidized LDL-induced oxidative stress and inflammation in retinal pigment epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammad Almarhoun,Lincoln Biswas,Reem Hasaballah Alhasani,Aileen Wong,Gabriel Mbuta Tchivelekete,Xinzhi Zhou,Steven Patterson,Chris Bartholomew,Xinhua Shu

International journal of molecular sciences 22: PubMed33445576

2021

Protease Inhibitor Anti-HIV, Lopinavir, Impairs Placental Endocrine Function.

Applications

Unspecified application

Species

Unspecified reactive species

Camille Fraichard,Fidéline Bonnet-Serrano,Christelle Laguillier-Morizot,Marylise Hebert-Schuster,René Lai-Kuen,Jeanne Sibiude,Thierry Fournier,Marie Cohen,Jean Guibourdenche

Nanomaterials (Basel, Switzerland) 10: PubMed32635405

2020

Uptake of Cerium Dioxide Nanoparticles and Impact on Viability, Differentiation and Functions of Primary Trophoblast Cells from Human Placenta.

Applications

Unspecified application

Species

Unspecified reactive species

Margaux Nedder,Sonja Boland,Stéphanie Devineau,Amal Zerrad-Saadi,Jasmina Rogozarski,René Lai-Kuen,Ibtissem Baya,Jean Guibourdenche,Francoise Vibert,Audrey Chissey,Sophie Gil,Xavier Coumoul,Thierry Fournier,Ioana Ferecatu

Molecular and cellular endocrinology 499:110586 PubMed31539598

2019

Placental production of progestins is fully effective in villous cytotrophoblasts and increases with the syncytiotrophoblast formation.

Applications

Unspecified application

Species

Unspecified reactive species

C Fraichard,F Bonnet,A Garnier,M Hébert-Schuster,A Bouzerara,P Gerbaud,I Ferecatu,T Fournier,I Hernandez,S Trabado,J Guibourdenche

Biochemical and biophysical research communication 505:1228-1235 PubMed30322615

2018

MLN64 deletion suppresses RANKL-induced osteoclastic differentiation and attenuates diabetic osteoporosis in streptozotocin (STZ)-induced mice.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Lei,Han Xueming,Duan Ruihang

The Journal of biological chemistry 293:7355-7366 PubMed29593096

2018

Myristoylated methionine sulfoxide reductase A is a late endosomal protein.

Applications

Unspecified application

Species

Unspecified reactive species

Jung Mi Lim,Jung Chae Lim,Geumsoo Kim,Rodney L Levine
View all publications
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