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AB327259

Anti-MLX Antibody

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Rabbit Polyclonal MLX antibody. Suitable for WB, ICC/IF, ChIP and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MLX.

View Alternative Names

BHLHD13, TCFL4, MLX, Max-like protein X, Class D basic helix-loop-helix protein 13, Max-like bHLHZip protein, Protein BigMax, Transcription factor-like protein 4, bHLHd13

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-MLX Antibody (AB327259)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MLX Antibody (AB327259)

Immunofluorescent staining of human cell line MCF7 using ab327259 at 0.25–2 µg/ml, showing localization to nucleoplasm, nuclear membrane & cytosol.

ChIP - Anti-MLX Antibody (AB327259)
  • ChIP

Supplier Data

ChIP - Anti-MLX Antibody (AB327259)

ChIP-Exo-Seq composite graph for ab327259 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Western blot - Anti-MLX Antibody (AB327259)
  • WB

Supplier Data

Western blot - Anti-MLX Antibody (AB327259)

Western blot analysis in human cell line RT-4 and human cell line U-251 MG using ab327259 at 0.04–0.4 µg/ml.

All lanes:

Western blot - Anti-MLX Antibody (ab327259)

Lane 2:

Human cell line RT-4

Lane 3:

Human cell line U-251 MG

Predicted band size: 33.3 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, ChIP

applications

Immunogen

Recombinant Fragment Protein within Human MLX.

Q9UH92

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "1-10 µg", "ChIP-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MAD1, MAD4, MNT, WBSCR14 and MLXIP which recognizes the core sequence 5'-CACGTG-3'. The TCFL4-MAD1, TCFL4-MAD4, TCFL4-WBSCR14 complexes are transcriptional repressors. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation.
See full target information MLX

Product promise

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