JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB234879

Anti-MLYCD/MCD antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal MLYCD/MCD antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MLYCD.

View Alternative Names

MCD, MLYCD

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-MLYCD/MCD antibody (AB234879)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MLYCD/MCD antibody (AB234879)

HepG2 (human liver hepatocellular carcinoma cell line) cells labeling MLYCD/MCD (green) using ab234879 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLYCD/MCD antibody (AB234879)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLYCD/MCD antibody (AB234879)

Paraffin-embedded human heart tissue stained for MLYCD/MCD using ab234879 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-MLYCD/MCD antibody (AB234879)
  • WB

Supplier Data

Western blot - Anti-MLYCD/MCD antibody (AB234879)

All lanes:

Western blot - Anti-MLYCD/MCD antibody (ab234879) at 1/2000 dilution

All lanes:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 55 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human MLYCD.

O95822

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MLYCD protein also known as malonyl-CoA decarboxylase (MCD) is an enzyme with a molecular mass of approximately 55 kDa. It is primarily located in the cytosol mitochondria and peroxisomes of cells. MLYCD functions by decarboxylating malonyl-CoA to acetyl-CoA and carbon dioxide playing an important role in the regulation of fatty acid metabolism and energy production.
Biological function summary

MLYCD converts malonyl-CoA an inhibitor of mitochondrial fatty acid oxidation into acetyl-CoA. This conversion permits fatty acids to enter mitochondria for beta-oxidation. It is not typically part of larger protein complexes but can interact with other molecules regulating lipid metabolism. Through these interactions it indirectly influences cellular energy balance and lipid biosynthesis.

Pathways

MLYCD is involved in the fatty acid metabolism pathway and energy homeostasis. Within these it serves as a regulatory point influencing the balance between fat utilization and storage. MLYCD maintains balance alongside proteins such as carnitine palmitoyltransferase 1A (CPT1A) which controls the entry of fatty acids into mitochondria for beta-oxidation linking these pathways.

MLYCD connects to metabolic conditions like malonyl-CoA decarboxylase deficiency and Type 2 diabetes. In malonyl-CoA decarboxylase deficiency reduced activity of MLYCD leads to the accumulation of malonyl-CoA affecting the energy metabolism. In Type 2 diabetes dysregulation of MLYCD can influence insulin sensitivity and lipid metabolism. Its role makes it critical for understanding metabolic health and disease with connections to proteins such as CPT1A and ACC (acetyl-CoA carboxylase) in these contexts.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Catalyzes the conversion of malonyl-CoA to acetyl-CoA. In the fatty acid biosynthesis MCD selectively removes malonyl-CoA and thus assures that methyl-malonyl-CoA is the only chain elongating substrate for fatty acid synthase and that fatty acids with multiple methyl side chains are produced. In peroxisomes it may be involved in degrading intraperoxisomal malonyl-CoA, which is generated by the peroxisomal beta-oxidation of odd chain-length dicarboxylic fatty acids. Plays a role in the metabolic balance between glucose and lipid oxidation in muscle independent of alterations in insulin signaling. May play a role in controlling the extent of ischemic injury by promoting glucose oxidation.
See full target information MLYCD
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer research 83:3920-3939 PubMed37729394

2023

Fatty Acid Oxidation Mediated by Malonyl-CoA Decarboxylase Represses Renal Cell Carcinoma Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Lijie Zhou,Yongbo Luo,Yuenan Liu,Youmiao Zeng,Junwei Tong,Mengting Li,Yaxin Hou,Kaixuan Du,Yabin Qi,Wenbang Pan,Yuanhao Liu,Rongli Wang,Fengyan Tian,Chaohui Gu,Ke Chen

Cell 185:3356-3374.e22 PubMed36055199

2022

Sublethal cytochrome c release generates drug-tolerant persister cells.

Applications

Unspecified application

Species

Unspecified reactive species

Halime Kalkavan,Mark J Chen,Jeremy C Crawford,Giovanni Quarato,Patrick Fitzgerald,Stephen W G Tait,Colin R Goding,Douglas R Green
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB236868

Anti-SCD1 antibody [EPR21963]

KO Validated|RabMAb|Recombinant|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-SCD1 antibody [EPR21963] (AB236868)

  • 0
  • 0
Primary Antibodies

AB124945

Anti-IRE1 (phospho S724) antibody [EPR5253]

RabMAb|Recombinant

Western blot - Anti-IRE1 (phospho S724) antibody [EPR5253] (AB124945)

  • 4
  • 8
Primary Antibodies

AB207327

Anti-Albumin antibody [EPR20195]

RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-Albumin antibody [EPR20195] (AB207327)

  • 5
  • 2
Primary Antibodies

AB134988

Anti-CPT1B antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT1B antibody (AB134988)

  • 5
  • 4

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com