JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB174831

Anti-MMAB antibody [EPR8695]

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Recombinant Monoclonal MMAB antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

View Alternative Names

Corrinoid adenosyltransferase MMAB, ATP:co(I)rrinoid adenosyltransferase MMAB, Methylmalonic aciduria type B protein, MMAB

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-MMAB antibody [EPR8695] (AB174831)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MMAB antibody [EPR8695] (AB174831)

Immunofluorescent analysis of HepG2 cells labeling MMAB with ab174831 at 1/50 dilution (green). DAPI nuclear staining (blue).

Western blot - Anti-MMAB antibody [EPR8695] (AB174831)
  • WB

Supplier Data

Western blot - Anti-MMAB antibody [EPR8695] (AB174831)

All lanes:

Western blot - Anti-MMAB antibody [EPR8695] (ab174831) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

Human fetal liver tissue lysate at 10 µg

Lane 3:

A673 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 27 kDa

true

  • Carrier free

    Anti-MMAB antibody [EPR8695] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8695

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MMAB also known as methylmalonic aciduria type B protein plays a critical role in the conversion of certain molecules within the mitochondria. This protein weighing approximately 38 kDa assists in the conversion of vitamin B12 into adenosylcobalamin an active coenzyme form. MMAB is present in many tissues with significant expression in liver and kidney where significant mitochondrial activities occur. Methylmalonyl-CoA a byproduct needing processing for energy production relies on MMAB for transformation.
Biological function summary

MMAB is indispensable for vitamin B12 metabolism and mitochondrial function. This protein acts within a larger enzymatic complex that converts methylmalonyl-CoA to succinyl-CoA. By playing a role in this conversion MMAB facilitates the Krebs cycle an essential biochemical pathway for energy production. Its interaction with other proteins like methylmalonyl-CoA mutase ensures proper biochemical transformations highlighting its importance in metabolic processes.

Pathways

Methylmalonic acidemia results from disruptions in the MMAB-mediated pathway notably affecting energy metabolism. This protein integrates into the mitochondrial pathway that handles odd-chain fatty acids and amino acid breakdown. The effective collaboration of MMAB with methylmalonyl-CoA mutase ensures efficient functioning within these pathways linking MMAB to essential metabolic activities like gluconeogenesis and energy production.

Mutations in MMAB contribute to methylmalonic acidemia a metabolic disorder characterized by accumulation of methylmalonic acid in the body. This condition can lead to developmental delay metabolic crisis and other severe symptoms if untreated. Alongside methylmalonyl-CoA mutase MMAB mutations impact vitamin B12-related pathways highlighting their joint role in this genetic disorder. Early diagnosis and treatment targeting this pathway help mitigate symptoms related to these enzymatic dysfunctions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Converts cob(I)alamin to adenosylcobalamin (adenosylcob(III)alamin), a coenzyme for methylmalonyl-CoA mutase, therefore participates in the final step of the vitamin B12 conversion (PubMed : 12514191). Generates adenosylcobalamin (AdoCbl) and directly delivers the cofactor to MUT in a transfer that is stimulated by ATP-binding to MMAB and gated by MMAA (Probable).
See full target information MMAB
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:527 PubMed40664675

2025

Simvastatin suppresses spinal cord metastasis of medulloblastoma at clinically significant doses.

Applications

Unspecified application

Species

Unspecified reactive species

Charley Comer,Kian Cotton,Christopher Edwards,Xiaoyang Dai,Sara Badodi,Roberto Buccafusca,Chris Bennett,Andrew Peet,Alice Williams,David Michod,Elena Bochukova,Maria Victoria Niklison-Chirou

Nature metabolism 4:435-443 PubMed35361954

2022

Altered propionate metabolism contributes to tumour progression and aggressiveness.

Applications

Unspecified application

Species

Unspecified reactive species

Ana P Gomes,Didem Ilter,Vivien Low,Stanislav Drapela,Tanya Schild,Edouard Mullarky,Julie Han,Ilaria Elia,Dorien Broekaert,Adam Rosenzweig,Michal Nagiec,Joana B Nunes,Bethany E Schaffer,Anders P Mutvei,John M Asara,Lewis C Cantley,Sarah-Maria Fendt,John Blenis
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB243596

Anti-MMAB antibody

Immunocytochemistry/ Immunofluorescence - Anti-MMAB antibody (AB243596)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB134956

Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (AB134956)

  • 5
  • 1
Primary Antibodies

AB76149

Anti-YB1 antibody [EP2708Y]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YB1 antibody [EP2708Y] (AB76149)

  • 5
  • 3
Primary Antibodies

AB250831

Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] - BSA and Azide free (AB250831)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com