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AB92368

Anti-mMCP-4 antibody

4

(1 Review)

|

(6 Publications)

Goat Polyclonal mMCP-4 antibody. Suitable for IHC-P and reacts with Mouse samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Mouse Mcpt4 aa 100-150.

View Alternative Names

Mast cell protease 4, mMCP-4, MSMCP, Myonase, Serosal mast cell protease, Mcpt4

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Mouse Mcpt4 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

P21812

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Complementary Products

Primary Antibodies

AB2378

Anti-Mast Cell Tryptase antibody [AA1]

20ul selling size|BOND RX™ Validated|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (AB2378)

  • 4
  • 35
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
Primary Antibodies

AB2377

Anti-Mast Cell Chymase antibody [CC1]

BOND RX™ Validated|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (AB2377)

  • 2
  • 3
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab92368 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MMCP-4 known as mouse mast cell protease 4 is a chymase encoded by the Mcpt4 gene. This serine protease has an approximate molecular mass of 30 kDa. mMCP-4 expresses mainly in connective tissue-type mast cells where it plays an important mechanical role. It selectively cleaves peptide bonds aiding in the breakdown of proteins and peptides contributing to various cellular processes. The protease operates in specific tissues such as the skin intestines and respiratory tracts where mast cells abundantly exist.
Biological function summary

Mouse mast cell protease 4 contributes to immune response regulation. It forms part of a complex mast cell granule where it collaborates with other enzymes to facilitate inflammation and immune defense mechanisms. Through proteolytic activity mMCP-4 regulates inflammatory mediators and impacts processes like vascular permeability and tissue remodeling. Its enzymatic function enables it to degrade extracellular matrix components and activate other cells in the immune system.

Pathways

MMCP-4 participates in the immune and inflammatory pathways. It directly interacts with components of the immune system by affecting cytokine release and modulating other mast cell products. mMCP-4 operates alongside proteins like tryptase and chymase family proteins contributing to processes such as histamine release and cytokine metabolism. These interactions allow efficient coordination within immune pathways leading to effective cellular responses against pathogens.

Its role in mast cell-driven inflammation connects mMCP-4 to conditions like asthma and atopic dermatitis. Its protease function influences the pathology of these inflammatory disorders by regulating mediators that exacerbate symptoms. Through these disease pathways mMCP-4 interacts with proteins such as matrix metalloproteinases and other related chymases affecting disease progression and severity. Understanding mMCP-4 is important for developing therapeutic approaches targeting mast cell-mediated inflammation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Has chymotrypsin-like activity. Hydrolyzes the amide bonds of synthetic substrates having Tyr and Phe residues at the P1 position. Preferentially hydrolyzes the 'Tyr-4-|-Ile-5' bond of angiotensin I and the 'Phe-20-|-Ala-21' bond of amyloid beta-protein, and is less active towards the 'Phe-8-|-His-9' bond of angiotensin I and the 'Phe-4-|-Ala-5' and 'Tyr-10-|-Glu-11' bonds of amyloid beta-protein. Involved in thrombin regulation and fibronectin processing.
See full target information Mcpt4
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40332817

2025

Chymase Inhibition Attenuates Kidney Fibrosis in a Chronic Mouse Model of Renal Ischemia-Reperfusion Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Sakura Kure,Hiroe Toba,Denan Jin,Akira Mima,Shinji Takai

Nature communications 14:7349 PubMed37963864

2023

TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase.

Applications

Unspecified application

Species

Unspecified reactive species

Gang Liu,Tatt Jhong Haw,Malcolm R Starkey,Ashleigh M Philp,Stelios Pavlidis,Christina Nalkurthi,Prema M Nair,Henry M Gomez,Irwan Hanish,Alan Cy Hsu,Elinor Hortle,Sophie Pickles,Joselyn Rojas-Quintero,Raul San Jose Estepar,Jacqueline E Marshall,Richard Y Kim,Adam M Collison,Joerg Mattes,Sobia Idrees,Alen Faiz,Nicole G Hansbro,Ryutaro Fukui,Yusuke Murakami,Hong Sheng Cheng,Nguan Soon Tan,Sanjay H Chotirmall,Jay C Horvat,Paul S Foster,Brian Gg Oliver,Francesca Polverino,Antonio Ieni,Francesco Monaco,Gaetano Caramori,Sukhwinder S Sohal,Ken R Bracke,Peter A Wark,Ian M Adcock,Kensuke Miyake,Don D Sin,Philip M Hansbro

The journal of physiological sciences : JPS 73:24 PubMed37828465

2023

Connective tissue mast cells store and release noradrenaline.

Applications

Unspecified application

Species

Unspecified reactive species

Yusuke Otani,Soichiro Yoshikawa,Kei Nagao,Takehiro Tanaka,Shinichi Toyooka,Atsushi Fujimura

International journal of molecular sciences 21: PubMed33050674

2020

Mechanism of Albuminuria Reduction by Chymase Inhibition in Diabetic Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kentaro Terai,Denan Jin,Kenji Watase,Akihisa Imagawa,Shinji Takai

Proceedings of the National Academy of Sciences of 117:23751-23761 PubMed32917815

2020

Perinatal androgens organize sex differences in mast cells and attenuate anaphylaxis severity into adulthood.

Applications

Unspecified application

Species

Unspecified reactive species

Emily Mackey,Kyan M Thelen,Vedrana Bali,Mahsa Fardisi,Madalyn Trowbridge,Cynthia L Jordan,Adam J Moeser

Nature communications 9:4559 PubMed30455465

2018

H3K9 methyltransferases and demethylases control lung tumor-propagating cells and lung cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

S P Rowbotham,F Li,A F M Dost,S M Louie,B P Marsh,P Pessina,C R Anbarasu,C F Brainson,S J Tuminello,A Lieberman,S Ryeom,T M Schlaeger,B J Aronow,H Watanabe,K K Wong,C F Kim
View all publications
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Product promise

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