Rabbit Polyclonal MMP1 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 71 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MMP1 aa 1-250.
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- Journal of nanobiotechnology 21:3502023Programmable DNA hydrogel provides suitable microenvironment for enhancing autophagy-based therapies in intervertebral disc degeneration treatment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSong Qingxin,Jiang Kai,Zheng Dandan,Jin Linyu,Chen Xiuyuan,Feng Yubo,Wang Kun,Han Yingchao,Chen Hao,Song Jie,Chen Zhi,Shen HongxingPubMed 37759249
- Preventive nutrition and food science 28:43-492023Chondroprotective Effects of on Osteoarthritis through MAPKs Signaling Inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSeul Ah Lee,Seul Hee Han,Ji Yun Jang,Bo-Ram Park,Chun Sung KimPubMed 37066028
- Bioengineering & translational medicine 8:e104392023Fibrotic remodeling and tissue regeneration mechanisms define the therapeutic potential of human muscular progenitors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYa-Chuan Hsiao,I-Han Wang,Tsung-Lin YangPubMed 36925693
- Computational and mathematical methods in medicine 2022:30583422022MMP1 Overexpression Promotes Cancer Progression and Associates with Poor Outcome in Head and Neck Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Zhang,Xinghong Huang,Rong Huang,Hong Zhu,Pu Ye,Xuyang Lin,Shuangyue Zhang,Meng Wu,Feng JiangPubMed 36105241
- Hernia : the journal of hernias and abdominal wall surgery 26:1695-17052022Similar collagen distribution in full-thickness skin grafts in intraperitoneal and onlay positions, an experimental mice-study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesA Winsnes,M-L Ivarsson,P Falk,U Gunnarsson,K StrigårdPubMed 36048398
- Scientific reports 12:119572022Glutamine exerts a protective effect on osteoarthritis development by inhibiting the Jun N-terminal kinase and nuclear factor kappa-B signaling pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLin Zhong,Le Cao,Rui Song,Xue-Fei Yang,Jia-Le Li,Hai-Tao Yang,Hong-Xiang Zhou,Hai-Tao FanPubMed 35831464
- Frontiers in cellular and infection microbiology 12:8972912022Treating 'Septic' With Enhanced Antibiotics and 'Arthritis' by Mitigation of Excessive Inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyuk-Kwon Kwon,Christopher M Dussik,Sang-Hun Kim,Themis R Kyriakides,Irvin Oh,Francis Y LeePubMed 35755835
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 36:e222582022Blocking chondrocyte hypertrophy in conditional Evc knockout mice does not modify cartilage damage in osteoarthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAna Lamuedra,Paula Gratal,Lucía Calatrava,Víctor Luis Ruiz-Perez,Adrián Palencia-Campos,Sergio Portal-Núñez,Aránzazu Mediero,Gabriel Herrero-Beaumont,Raquel LargoPubMed 35334131
- Experimental and therapeutic medicine 23:3372022Ketorolac tromethamine alleviates IL-1β-induced chondrocyte injury by inhibiting COX-2 expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChang Liu,Yizhi ChenPubMed 35411209
- mBio :e03144212022Candida albicans Enhances the Progression of Oral Squamous Cell Carcinoma and .Applications:Unspecified applicationReactive species:Unspecified reactive speciesMáté Vadovics,Jemima Ho,Nóra Igaz,Róbert Alföldi,Dávid Rakk,Éva Veres,Balázs Szücs,Márton Horváth,Renáta Tóth,Attila Szücs,Andrea Csibi,Péter Horváth,László Tiszlavicz,Csaba Vágvölgyi,Joshua D Nosanchuk,András Szekeres,Mónika Kiricsi,Rhonda Henley-Smith,David L Moyes,Selvam Thavaraj,Rhys Brown,László G Puskás,Julian R Naglik,Attila GácserPubMed 35089096
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human MMP1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link P03956
Consider this alternative
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Not recommended | Expected |
| Mouse | Predicted | Not recommended | Expected |
| Pig | Predicted | Not recommended | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes Blocking in 5% non-fat milk in TBST for 60 minutes at room temperature. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig, Mouse | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Pig | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X (PubMed:1645757, PubMed:2153297, PubMed:2557822). In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity (PubMed:16807369).
Alternative names
CLG, CLG, MMP1, Interstitial collagenase, Fibroblast collagenase, Matrix metalloproteinase-1, MMP-1
Recommended products
Rabbit Polyclonal MMP1 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 71 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MMP1 aa 1-250.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human MMP1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link P03956
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MMP1 also known as collagenase-1 is an enzyme belonging to the matrix metalloproteinase (MMP) family. It is responsible for the degradation of type I II and III collagens making it an important player in collagen turnover. MMP1 has a molecular weight of approximately 54 kDa. This enzyme is expressed in various tissues including skin lung and reproductive organs primarily during tissue remodeling and repair. Scientists often use MMP1 ELISA kits and MMP1 inhibitors to study its expression and activity levels.
- Biological function summary
MMP1 plays a significant role in the modulation of the extracellular matrix. It facilitates cellular processes by breaking down structural proteins and allowing for cell migration proliferation and differentiation. Although it acts independently MMP1 works in harmony with other MMP family members to maintain extracellular matrix homeostasis and is often linked to tissue remodeling complexes. This makes the MMP1 a valuable target for researchers interested in tissue repair and fibrosis.
- Pathways
Studies show MMP1 involvement in critical processes such as the inflammatory response and wound healing. In the inflammatory pathway MMP1 expression is regulated by cytokines that respond to tissue injury. Within the wound healing pathway this enzyme interacts with proteins like transforming growth factor-beta (TGF-beta) to coordinate the repair process by remodeling extracellular matrix components. This illustrates the interconnected nature of MMP1 with significant regulatory proteins.
- Associated diseases and disorders
MMP1 has significant associations with osteoarthritis and cancer metastasis. MMP1 contributes to the breakdown of cartilage in osteoarthritis highlighting its role in disease progression and joint degradation. In the context of cancer elevated MMP1 expression correlates with the metastatic potential where it assists tumor invasion by degrading the surrounding matrix. MMP1's involvement with other MMPs including MMP2 and MMP9 highlights its importance in these pathological conditions making it a therapeutic target in related research.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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4 product images
Western blot - Anti-MMP1 antibody (ab137332)
10% SDS gel
Running condition: 80V, 15min; 140V, 40 min
Primary antibody incubation: 4C overnight
Washing condition: 5 mL TBST, 4 x 5 minutesAll lanes: Western blot - Anti-MMP1 antibody (ab137332) at 1/2500 dilution
All lanes: HUVEC (human umbilical vein endothelial cell line) whole cell extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG at 1/10000 dilution
Predicted band size: 54 kDa
Western blot - Anti-MMP1 antibody (ab137332)
All lanes: Western blot - Anti-MMP1 antibody (ab137332) at 1/1000 dilution
All lanes: Raji (Human Burkitt's lymphoma cell line) whole cell lysate at 30 µg
Predicted band size: 54 kDa
Western blot - Anti-MMP1 antibody (ab137332)
Low expression: A549, HT-29, IMR-32.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 25319362).
Anti-MMP1 antibody (Anti-MMP1 antibody [RM1206] ab318207, Left) staining at 1/1000 dilution showed similar expression profile/ molecular weight with Anti-MMP1 antibody (ab137332, Right) at 1/1000 dilution.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-MMP1 antibody [RM1206] (Anti-MMP1 antibody [RM1206] ab318207) at 1/1000 dilution
Lane 1: Untreated HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: HeLa treated with 80 nM TPA for 24 hours, then replaced with 10 ug /ml poly(dA:dT) for 2 hours, whole cell lysate at 20 µg
Lane 3: HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg
Lane 4: A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 5: HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 6: IMR-32 (human neuroblastoma neuroblast) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 54 kDa, 36 kDa
Exposure time: 180s
Immunoprecipitation - Anti-MMP1 antibody (ab137332)
MMP1 was immunoprecipitated from 0.35 mg HUVEC (human umbilical vein endothelial cell) whole cell lysate with Anti-MMP1 antibody [RM1206] ab318207 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-MMP1 antibody [RM1206] ab318207 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HUVEC (human umbilical vein endothelial cell) whole cell lysate
Lane 2: Anti-MMP1 antibody [RM1206] ab318207 IP in HUVEC (human umbilical vein endothelial cell) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-MMP1 antibody [RM1206] ab318207 in HUVEC whole cell lysateAll lanes: Immunoprecipitation - Anti-MMP1 antibody [RM1206] (Anti-MMP1 antibody [RM1206] ab318207) at 1/30 dilution
All lanes: HUVEC (human umbilical vein endothelial cell) whole cell lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 6s
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