Anti-MMP1 antibody

• WB

Supplier Data

Western blot - Anti-MMP1 antibody (AB137332)

Low expression : A549, HT-29, IMR-32.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 25319362).

Anti-MMP1 antibody (ab318207, Left) staining at 1/1000 dilution showed similar expression profile/ molecular weight with Anti-MMP1 antibody (ab137332, Right) at 1/1000 dilution.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-MMP1 antibody [RM1206] (<a href='/en-us/products/primary-antibodies/mmp1-antibody-rm1206-ab318207'>ab318207</a>) at 1/1000 dilution

Lane 1:

Untreated HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HeLa treated with 80 nM TPA for 24 hours, then replaced with 10 ug /ml poly(dA:dT) for 2 hours, whole cell lysate at 20 µg

Lane 3:

HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg

Lane 4:

A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 6:

IMR-32 (human neuroblastoma neuroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 54 kDa,36 kDa

false

Exposure time: 180s

• IP

Supplier Data

Immunoprecipitation - Anti-MMP1 antibody (AB137332)

MMP1 was immunoprecipitated from 0.35 mg HUVEC (human umbilical vein endothelial cell) whole cell lysate with ab318207 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab318207 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HUVEC (human umbilical vein endothelial cell) whole cell lysate
Lane 2 : ab318207 IP in HUVEC (human umbilical vein endothelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab318207 in HUVEC whole cell lysate

All lanes:

Immunoprecipitation - Anti-MMP1 antibody [RM1206] (<a href='/en-us/products/primary-antibodies/mmp1-antibody-rm1206-ab318207'>ab318207</a>) at 1/30 dilution

All lanes:

HUVEC (human umbilical vein endothelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 6s

• WB

Supplier Data

Western blot - Anti-MMP1 antibody (AB137332)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-MMP1 antibody (ab137332) at 1/2500 dilution

All lanes:

HUVEC Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 50 kDa

false