Skip to main content

Rabbit Recombinant Monoclonal MMP1 antibody. Suitable for Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Human samples. Cited in 60 publications.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody [EP1247Y] (AB52631), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody [EP1247Y] (AB52631), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody [EP1247Y] (AB52631), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody [EP1247Y] (AB52631), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MMP1 antibody [EP1247Y] (AB52631), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow Cyt (Intra)WBICC/IFIHC-P
Human
Not recommended
Tested
Not recommended
Tested
Tested

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/20

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

4 products for Alternative Product

1 product for Alternative Version

Target data

Function

Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X (PubMed:1645757, PubMed:2153297, PubMed:2557822). In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity (PubMed:16807369).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal MMP1 antibody. Suitable for Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Human samples. Cited in 60 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EP1247Y

Purification technique

Affinity purification Protein A

Specificity

This antibody is able to detect recombinant protein in western blot but it failed to detect the endogenous protein. Therefore, we do not recommend the antibody in this application. For western blot application we recommend using ab134184.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody [EP1247Y] (ab52631), expandable thumbnail
    This image is courtesy of an anonymous customer review.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody [EP1247Y] (ab52631)

    Formaldehyde-fixed, paraffin-embedded human placenta tissue stained for MMP1 using ab52631 at 1/40 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody [EP1247Y] (ab52631), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody [EP1247Y] (ab52631)

    Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of cervix tissue labeling MMP1 with ab52631 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051, 1/500). Counterstained with hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody [EP1247Y] (ab52631), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody [EP1247Y] (ab52631)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling MMP1 with unpurified ab52631 at 1/60. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    IHC result showed parenchymal cells (such as spermatogonium and spermatocytes) in seminiferous tubules were negative, and the stromal cells were stained.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody [EP1247Y] (ab52631), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody [EP1247Y] (ab52631)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling MMP1 with purified ab52631 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    IHC result showed parenchymal cells (such as spermatogonium and spermatocytes) in seminiferous tubules were negative, and the stromal cells were stained.

  • Immunocytochemistry/ Immunofluorescence - Anti-MMP1 antibody [EP1247Y] (ab52631), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MMP1 antibody [EP1247Y] (ab52631)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling MMP1 with unpurified ab52631 at 1/30. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

    Control: primary antibody (1/30) and secondary antibody, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

  • Immunocytochemistry/ Immunofluorescence - Anti-MMP1 antibody [EP1247Y] (ab52631), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MMP1 antibody [EP1247Y] (ab52631)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling MMP1 with purified ab52631 at 1/50. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

    Control: primary antibody (1/50) and secondary antibody, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

  • Flow Cytometry (Intracellular) - Anti-MMP1 antibody [EP1247Y] (ab52631), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-MMP1 antibody [EP1247Y] (ab52631)

    Flow cytometry analysis of HeLa cells labelling MMP1 with unpurified ab52631 at 1/50 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.

  • Flow Cytometry (Intracellular) - Anti-MMP1 antibody [EP1247Y] (ab52631), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-MMP1 antibody [EP1247Y] (ab52631)

    Flow cytometry analysis of HeLa cells labelling MMP1 with purified ab52631 at 1/70 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG was used as the secondary antibody (1/150). Green - Isotype control, rabbit monoclonal IgG.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody [EP1247Y] (ab52631), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody [EP1247Y] (ab52631)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue labelling MMP1 with ab52631 at 1/50.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-MMP1 antibody [EP1247Y] (ab52631), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MMP1 antibody [EP1247Y] (ab52631)

    ICC/IF image of unpurified ab52631 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52631, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com