Anti-MMP1 antibody [EP1247Y] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody [EP1247Y] - BSA and Azide free (AB271845)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue labelling MMP1 with ab52631 at 1/50.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52631).

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-MMP1 antibody [EP1247Y] - BSA and Azide free (AB271845)

Intracellular Flow Cytometry analysis of HeLa cells labelling MMP1 with purified ab52631 at 1/70 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG was used as the secondary antibody (1/150). Green - Isotype control, rabbit monoclonal IgG.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52631).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MMP1 antibody [EP1247Y] - BSA and Azide free (AB271845)

ICC/IF image of unpurified ab52631 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52631, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor^® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor^® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52631).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody [EP1247Y] - BSA and Azide free (AB271845)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling MMP1 with unpurified ab52631 at 1/60. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

IHC result showed parenchymal cells (such as spermatogonium and spermatocytes) in seminiferous tubules were negative, and the stromal cells were stained.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52631).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MMP1 antibody [EP1247Y] - BSA and Azide free (AB271845)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling MMP1 with unpurified ab52631 at 1/30. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor^® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

Control : primary antibody (1/30) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52631).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MMP1 antibody [EP1247Y] - BSA and Azide free (AB271845)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling MMP1 with purified ab52631 at 1/50. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor^® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

Control : primary antibody (1/50) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52631).

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-MMP1 antibody [EP1247Y] - BSA and Azide free (AB271845)

Intracellular Flow Cytometry analysis of HeLa cells labelling MMP1 with unpurified ab52631 at 1/50 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52631).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody [EP1247Y] - BSA and Azide free (AB271845)

Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of cervix tissue labeling MMP1 with ab52631 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051, 1/500). Counterstained with hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52631).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody [EP1247Y] - BSA and Azide free (AB271845)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling MMP1 with purified ab52631 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

IHC result showed parenchymal cells (such as spermatogonium and spermatocytes) in seminiferous tubules were negative, and the stromal cells were stained.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52631).