AB134184

Anti-MMP1 antibody [EP1249Y]

RabMAb
Recombinant
20ul selling size
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(32 Publications)

Rabbit Recombinant Monoclonal MMP1 antibody. Suitable for ICC/IF, IP, WB and reacts with Human samples. Cited in 32 publications.

View Alternative Names

CLG, MMP1, Interstitial collagenase, Fibroblast collagenase, Matrix metalloproteinase-1, MMP-1

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-MMP1 antibody [EP1249Y] (AB134184)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-MMP1 antibody [EP1249Y] (AB134184)

Immunocytochemistry/ Immunofluorescence analyis of HUVEC (human umbilical vein endothelial cell) labeling MMP1 with at ab134184 at 1/100 followed by ab150081 at 1/1000 dilution as the secondary antibody. ab195889 at 1/200 was used as counterstain. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100. Nuclear counterstain was DAPI (blue). Confocal image showing cytoplasmic staining in HUVEC cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Low expression : A549. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Lab

Immunoprecipitation - Anti-MMP1 antibody [EP1249Y] (AB134184)

MMP1 was immunoprecipitated from 0.35 mg MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10 μg with 134184 at 1/50 dilution (2μg). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10 μg

Lane 2 : ab134184 IP in MDA-MB-231 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab134184 in MDA-MB-231 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-MMP1 antibody [EP1249Y] (ab134184)

Predicted band size: 54 kDa

Observed band size: 54 kDa

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Unknown

Western blot - Anti-MMP1 antibody [EP1249Y] (AB134184)

All lanes:

Western blot - Anti-MMP1 antibody [EP1249Y] (ab134184) at 1/1000 dilution

All lanes:

A431 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 54 kDa

false

Unknown

Western blot - Anti-MMP1 antibody [EP1249Y] (AB134184)

Blocking and dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-MMP1 antibody [EP1249Y] (ab134184) at 1/500 dilution

Lane 1:

MDA-MB-231 (human breast adenocarcinoma epithelial) whole cell lysate at 10 µg

Lane 2:

Lysate from HeLa cells untreated with TPA at 10 µg

Lane 3:

HeLa treated with 100 ng/mL TPA for 10 hours cell lysate at 10 µg

Lane 4:

Human colon cancer tissue lysate at 10 µg

Lane 5:

Human breast cancer tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/1000 dilution

Predicted band size: 54 kDa

Observed band size: 54 kDa

false

Exposure time: 30s

Carrier free

Anti-MMP1 antibody [EP1249Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1249Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/20", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MMP1 also known as collagenase-1 is an enzyme belonging to the matrix metalloproteinase (MMP) family. It is responsible for the degradation of type I II and III collagens making it an important player in collagen turnover. MMP1 has a molecular weight of approximately 54 kDa. This enzyme is expressed in various tissues including skin lung and reproductive organs primarily during tissue remodeling and repair. Scientists often use MMP1 ELISA kits and MMP1 inhibitors to study its expression and activity levels.

Biological function summary

MMP1 plays a significant role in the modulation of the extracellular matrix. It facilitates cellular processes by breaking down structural proteins and allowing for cell migration proliferation and differentiation. Although it acts independently MMP1 works in harmony with other MMP family members to maintain extracellular matrix homeostasis and is often linked to tissue remodeling complexes. This makes the MMP1 a valuable target for researchers interested in tissue repair and fibrosis.

Pathways

Studies show MMP1 involvement in critical processes such as the inflammatory response and wound healing. In the inflammatory pathway MMP1 expression is regulated by cytokines that respond to tissue injury. Within the wound healing pathway this enzyme interacts with proteins like transforming growth factor-beta (TGF-beta) to coordinate the repair process by remodeling extracellular matrix components. This illustrates the interconnected nature of MMP1 with significant regulatory proteins.

MMP1 has significant associations with osteoarthritis and cancer metastasis. MMP1 contributes to the breakdown of cartilage in osteoarthritis highlighting its role in disease progression and joint degradation. In the context of cancer elevated MMP1 expression correlates with the metastatic potential where it assists tumor invasion by degrading the surrounding matrix. MMP1's involvement with other MMPs including MMP2 and MMP9 highlights its importance in these pathological conditions making it a therapeutic target in related research.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X (PubMed : 1645757, PubMed : 2153297, PubMed : 2557822). In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity (PubMed : 16807369).

See full target information MMP1

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Publications (32)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:27263 PubMed40715596

2025

Comparative and pharmacological investigation of bEVs from eight Lactobacillales strains.

Applications

Unspecified application

Species

Unspecified reactive species

Seoah Park,Jongsoo Mok,Hye-Min Yu,Hye-Jin An,Ga-Hyun Choi,Yeon-Seon Lee,Ki-Jin Kwon,Sung-Jun Choi,Kyung-Hee Kim,Soo-Jin Kim,Joonghoon Park

Frontiers in oncology 15:1568367 PubMed40666104

2025

Hsa_circ_0001859 promotes NSCLC progression through the miRNA-101-3p/MMP1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jianxin Tan,Zhenyu Fan,Rongguo Lu,Shugao Ye

Journal of ovarian research 18:124 PubMed40483481

2025

Silencing of STX4 inhibits the proliferation, migration and invasion of ovarian cancer cells via EMT/MMP2/ CCND1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wenfeng Ye,Chunyan Xue,Linlin Chen,Xiangnan Chen,Dachuan Zhang

Journal of virology 99:e0153924 PubMed39846742

2025

MA104 cell line is permissive for human bocavirus 1 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Jielin Tang,Sijie Chen,Yijun Deng,Junjun Liu,Dan Huang,Muqing Fu,Bao Xue,Canyu Liu,Chunchen Wu,Fan Wang,Yuan Zhou,Qi Yang,Xinwen Chen

The Journal of biological chemistry 300:107807 PubMed39307302

2024

Aberrant overexpression of myosin 1b in glioblastoma promotes angiogenesis via VEGF-myc-myosin 1b-Piezo1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Weifeng Lv,Fan Yang,Zhengmao Ge,Lele Xin,Lingxue Zhang,Yaohong Zhai,Xian Liu,Qingdong Guo,Xinggang Mao,Peng Luo,Lei Zhang,Xiaofan Jiang,Yanyu Zhang

Joint diseases and related surgery 35:513-520 PubMed39189559

2024

Estrogen receptor is involved in the osteoarthritis mediated by Atg16L1-NLRP3 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Fa-Xue Liao,Shuo Yang,Zhi-Hong Liu,Kai-Da Bo,Peng-Fei Xu,Jun Chang

Cancer letters 600:217180 PubMed39154702

2024

Single-cell RNA sequencing of OSCC primary tumors and lymph nodes reveals distinct origin and phenotype of fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Zhang,Liang Ding,Jingyi Li,Kunyu Liu,Chengwan Xia,Sheng Chen,Xiaofeng Huang,Yumei Pu,Yuxian Song,Qingang Hu,Yuxin Wang

Oncology letters 27:127 PubMed38333640

2024

Matrix metalloproteinase‑1 and microRNA‑486‑5p in urinary exosomes can be used to detect early lung cancer: A preliminary report.

Applications

Unspecified application

Species

Unspecified reactive species

Wataru Ando,Masaya Sogabe,Shigemi Ishikawa,Takayuki Uematsu,Hiroyuki Furuya,Hiroaki Yokomori,Yutaka Kohgo,Katsuya Otori,Tomoyuki Nakano,Shunsuke Endo,Hiroyoshi Tsubochi,Isao Okazaki

Journal of nanobiotechnology 21:354 PubMed37775761

2023

Bioinspired polydopamine nanoparticles as efficient antioxidative and anti-inflammatory enhancers against UV-induced skin damage.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Zhang,Yuqi Zhou,Zhaoting Jiang,Chenhui He,Bo Wang,Qi Wang,Zeqian Wang,Tong Wu,Xiaoqi Chen,Ziwei Deng,Chunying Li,Zhe Jian

International journal of molecular sciences 24: PubMed37446165

2023

Anti-Fibrotic Effects of RF Electric Currents.

Applications

Unspecified application

Species

Unspecified reactive species

María Luisa Hernández-Bule,Elena Toledano-Macías,Luis Alfonso Pérez-González,María Antonia Martínez-Pascual,Montserrat Fernández-Guarino

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MMP1 antibody [EP1249Y]

Immunocytochemistry/ Immunofluorescence - Anti-MMP1 antibody [EP1249Y] (AB134184)

Immunoprecipitation - Anti-MMP1 antibody [EP1249Y] (AB134184)

Western blot - Anti-MMP1 antibody [EP1249Y] (AB134184)

Western blot - Anti-MMP1 antibody [EP1249Y] (AB134184)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (32)

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