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Rabbit Recombinant Monoclonal MMP1 antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples.

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Images

Western blot - Anti-MMP1 antibody [EP1249Y] - BSA and Azide free (AB240086), expandable thumbnail
  • Western blot - Anti-MMP1 antibody [EP1249Y] - BSA and Azide free (AB240086), expandable thumbnail
  • Immunoprecipitation - Anti-MMP1 antibody [EP1249Y] - BSA and Azide free (AB240086), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MMP1 antibody [EP1249Y] - BSA and Azide free (AB240086), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFFlow Cyt (Intra)IPFlow CytWBIHC-P
Human
Tested
Not recommended
Tested
Not recommended
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse
Dilution info
-
Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X (PubMed:1645757, PubMed:2153297, PubMed:2557822). In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity (PubMed:16807369).

Alternative names

CLG, MMP1, Interstitial collagenase, Fibroblast collagenase, Matrix metalloproteinase-1, MMP-1

Recommended products

Rabbit Recombinant Monoclonal MMP1 antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EP1249Y
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab240086 is the carrier-free version of Anti-MMP1 antibody [EP1249Y] ab134184.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

MMP1 also known as collagenase-1 is an enzyme belonging to the matrix metalloproteinase (MMP) family. It is responsible for the degradation of type I II and III collagens making it an important player in collagen turnover. MMP1 has a molecular weight of approximately 54 kDa. This enzyme is expressed in various tissues including skin lung and reproductive organs primarily during tissue remodeling and repair. Scientists often use MMP1 ELISA kits and MMP1 inhibitors to study its expression and activity levels.

Biological function summary

MMP1 plays a significant role in the modulation of the extracellular matrix. It facilitates cellular processes by breaking down structural proteins and allowing for cell migration proliferation and differentiation. Although it acts independently MMP1 works in harmony with other MMP family members to maintain extracellular matrix homeostasis and is often linked to tissue remodeling complexes. This makes the MMP1 a valuable target for researchers interested in tissue repair and fibrosis.

Pathways

Studies show MMP1 involvement in critical processes such as the inflammatory response and wound healing. In the inflammatory pathway MMP1 expression is regulated by cytokines that respond to tissue injury. Within the wound healing pathway this enzyme interacts with proteins like transforming growth factor-beta (TGF-beta) to coordinate the repair process by remodeling extracellular matrix components. This illustrates the interconnected nature of MMP1 with significant regulatory proteins.

Associated diseases and disorders

MMP1 has significant associations with osteoarthritis and cancer metastasis. MMP1 contributes to the breakdown of cartilage in osteoarthritis highlighting its role in disease progression and joint degradation. In the context of cancer elevated MMP1 expression correlates with the metastatic potential where it assists tumor invasion by degrading the surrounding matrix. MMP1's involvement with other MMPs including MMP2 and MMP9 highlights its importance in these pathological conditions making it a therapeutic target in related research.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-MMP1 antibody [EP1249Y] - BSA and Azide free (ab240086), expandable thumbnail

    Western blot - Anti-MMP1 antibody [EP1249Y] - BSA and Azide free (ab240086)

    This data was developed using Anti-MMP1 antibody [EP1249Y] ab134184, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-MMP1 antibody [EP1249Y] (Anti-MMP1 antibody [EP1249Y] ab134184) at 1/500 dilution

    Lane 1: MDA-MB-231 (human breast adenocarcinoma epithelial) whole cell lysate at 10 µg

    Lane 2: Lysate from HeLa cells untreated with TPA at 10 µg

    Lane 3: HeLa treated with 100 ng/mL TPA for 10 hours cell lysate at 10 µg

    Lane 4: Human colon cancer tissue lysate at 10 µg

    Lane 5: Human breast cancer tissue lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG H&L (HRP) at 1/1000 dilution

    Predicted band size: 54 kDa

    Observed band size: 54 kDa

    Exposure time: 30s

  • Western blot - Anti-MMP1 antibody [EP1249Y] - BSA and Azide free (ab240086), expandable thumbnail

    Western blot - Anti-MMP1 antibody [EP1249Y] - BSA and Azide free (ab240086)

    This data was developed using Anti-MMP1 antibody [EP1249Y] ab134184, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-MMP1 antibody [EP1249Y] (Anti-MMP1 antibody [EP1249Y] ab134184) at 1/1000 dilution

    All lanes: A431 cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 54 kDa

  • Immunoprecipitation - Anti-MMP1 antibody [EP1249Y] - BSA and Azide free (ab240086), expandable thumbnail

    Immunoprecipitation - Anti-MMP1 antibody [EP1249Y] - BSA and Azide free (ab240086)

    This data was developed using Anti-MMP1 antibody [EP1249Y] ab134184, the same antibody clone in a different buffer formulation.MMP1 was immunoprecipitated from 0.35 mg MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10 µg with Anti-MMP1 antibody [EP1249Y] ab134184 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10 µg

    Lane 2: abab134184 IP in MDA-MB-231 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-MMP1 antibody [EP1249Y] ab134184 in MDA-MB-231 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-MMP1 antibody [EP1249Y] (Anti-MMP1 antibody [EP1249Y] ab134184)

    Predicted band size: 54 kDa

    Observed band size: 54 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-MMP1 antibody [EP1249Y] - BSA and Azide free (ab240086), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MMP1 antibody [EP1249Y] - BSA and Azide free (ab240086)

    This data was developed using Anti-MMP1 antibody [EP1249Y] ab134184, the same antibody clone in a different buffer formulation.

    Immunocytochemistry/ Immunofluorescence analyis of HUVEC (human umbilical vein endothelial cell) labeling MMP1 with at Anti-MMP1 antibody [EP1249Y] ab134184 at 1/100 followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 at 1/1000 dilution as the secondary antibody. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 at 1/200 was used as counterstain. Cells were fixed with 4% Paraformaldehyde and permeabilised with  0.1% TritonX-100. Nuclear counterstain was DAPI (blue).

    Confocal image showing cytoplasmic staining in HUVEC cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
    Low expression: A549.
    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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