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AB227238

Anti-MMP10 antibody

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Rabbit Polyclonal MMP10 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MMP10.

View Alternative Names

STMY2, MMP10, Stromelysin-2, SL-2, Matrix metalloproteinase-10, Transin-2, MMP-10

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP10 antibody (AB227238)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP10 antibody (AB227238)

Paraffin-embedded human gastric cancer tissue stained for MMP10 using ab227238 at 1/500 dilution in immunohistochemical analysis.

Western blot - Anti-MMP10 antibody (AB227238)
  • WB

Supplier Data

Western blot - Anti-MMP10 antibody (AB227238)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-MMP10 antibody (ab227238) at 1/1000 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg

Lane 2:

HepG2 (human liver hepatocellular carcinoma cell line) conditioned medium at 30 µg

Predicted band size: 54 kDa

false

Western blot - Anti-MMP10 antibody (AB227238)
  • WB

Supplier Data

Western blot - Anti-MMP10 antibody (AB227238)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-MMP10 antibody (ab227238) at 1/5000 dilution

Lane 1:

Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

MMP10-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Predicted band size: 54 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human MMP10. The exact immunogen used to generate this antibody is proprietary information.

P09238

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MMP10 also known as stromelysin-2 is a member of the matrix metalloproteinase (MMP) family. This protein is involved in the degradation of components of the extracellular matrix. MMP10 weighs around 54 kDa in its native form. It gets expressed in various tissues including the skin lung and reproductive organs and plays a part in processes like tissue remodeling and repair. Unlike other MMPs MMP10 does not usually cleave collagen but breaks down proteins like elastin and gelatin. The Luminex MMP assay is a method used to study this protein's expression and regulation.
Biological function summary

MMP10 impacts the restructuring and turnover of the extracellular matrix in both normal and disease conditions. It does not work alone; it operates synergistically with other MMPs in a complex matrix framework. Within tissues MMP10 modifies the integrity of the extracellular environment affecting cell behaviors such as migration and invasion. It steps in during processes like wound healing and inflammation exemplifying its contribution to cellular repair mechanisms and their regulation.

Pathways

The activity of MMP10 intersects with the MAPK and TGF-beta signaling pathways. MMP10 facilitates these pathways by digesting extracellular matrix barriers which then influences cellular responses. MMP10's function is also linked with other metalloproteinases like MMP1 and MMP3 in the pathways. Its regulation and activation play an important role in maintaining cellular homeostasis and responding to environmental cues.

MMP10 is associated with conditions such as cancer and arthritis. In cancer its role in promoting extracellular matrix breakdown facilitates tumor cell invasion and metastasis. In arthritis MMP10 contributes to joint tissue destruction perpetuating the inflammatory cycle. This protein frequently collaborates with MMP13 in these diseases reflecting its significant part in disease progression and pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase.
See full target information MMP10
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Product promise

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For full details, please see our Terms & Conditions

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