AB53143

Anti-MMP11 antibody

Name: Anti-MMP11 antibody (ab53143) | Abcam
Brand: Abcam Limited
SKU: AB53143
Rating: 4 (1 reviews)

(1 Review)

(12 Publications)

Rabbit Polyclonal MMP11 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human MMP11.

View Alternative Names

STMY3, MMP11, Stromelysin-3, SL-3, ST3, Matrix metalloproteinase-11, MMP-11

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP11 antibody (AB53143)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP11 antibody (AB53143)

ab53143 staining Human normal placenta. Staining is localised to the extracellular matrix (secreted).
Left panel : with primary antibody at 2 ug/ml. Right panel : isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be require

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Western blot - Anti-MMP11 antibody (AB53143)

All lanes:

Western blot - Anti-MMP11 antibody (ab53143) at 1/500 dilution

Lane 1:

A549 cell extract with no peptide

Lane 2:

A549 cell extract with peptide

Predicted band size: 55 kDa

Observed band size: 62 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human MMP11. The exact immunogen used to generate this antibody is proprietary information.

P24347

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MMP11 also known as stromelysin-3 is a matrix metalloproteinase with a mass of approximately 54 kDa. It participates in the breakdown of extracellular matrix components particularly during tissue remodeling and wound healing. MMP11 demonstrates unique mechanical behavior when compared to other MMPs as it prefers cleaving non-matrix substrates like alpha1-proteinase inhibitor. Expression levels of MMP11 vary but are commonly found in tissues undergoing active remodeling including the placenta and stroma of breast tumors.

Biological function summary

The modulation of the extracellular matrix by MMP11 helps in regulating tissue architecture and cellular interactions. Unlike other MMPs MMP11 does not participate in large protein complexes but acts mainly at the cellular boundaries. It helps facilitate tumor cell invasion by altering the local matrix environment and can modify growth factor activity aiding in cell proliferation and migration.

Pathways

MMP11 is deeply integrated into the tissue remodeling and invasion pathways. It associates with the serine protease pathway known for its role in regulating the extracellular environment. MMP11 is linked to proteins like TIMP1 which is a natural inhibitor and works together in maintaining the balance of extracellular matrix deposition and degradation.

Overexpression of MMP11 is frequently observed in various cancers such as breast and gastric cancer. Its contribution to tumor progression makes it a significant focus in cancer research. MMP11's interaction with other factors like insulin-like growth factor binding proteins influences cellular processes that compromise tissue integrity further linking it to cancer metastasis and progression. This relationship highlights its potential as a therapeutic target in oncology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May play an important role in the progression of epithelial malignancies.

See full target information MMP11

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:2152 PubMed39820824

2025

Comprehensive investigation of matrix metalloproteinases in skin cutaneous melanoma: diagnostic, prognostic, and therapeutic insights.

Applications

Unspecified application

Species

Unspecified reactive species

Lingxia Wu,Chenxiaoxiao Liu,Weicai Hu

Nature communications 15:10259 PubMed39592630

2024

Spatially organized tumor-stroma boundary determines the efficacy of immunotherapy in colorectal cancer patients.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Feng,Wenjuan Ma,Yupeng Zang,Yanying Guo,Young Li,Yixuan Zhang,Xuan Dong,Yi Liu,Xiaojuan Zhan,Zhizhong Pan,Mei Luo,Miaoqing Wu,Ao Chen,Da Kang,Gong Chen,Longqi Liu,Jingying Zhou,Rongxin Zhang

Cancer cell 42:396-412.e5 PubMed38242124

2024

Cancer-associated fibroblast phenotypes are associated with patient outcome in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lena Cords,Stefanie Engler,Martina Haberecker,Jan Hendrik Rüschoff,Holger Moch,Natalie de Souza,Bernd Bodenmiller

OncoTargets and therapy 13:11537-11547 PubMed33204108

2020

LncRNA CTBP1-AS2 Facilitates Gastric Cancer Progression via Regulating the miR-139-3p/MMP11 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yudan Yang,Ming Gao,Yunpeng Li,Mengyi Li,Qingqing Ma

Cancer management and research 12:10505-10519 PubMed33122949

2020

Circ_0000260 Regulates the Development and Deterioration of Gastric Adenocarcinoma with Cisplatin Resistance by Upregulating MMP11 via Targeting MiR-129-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Shicheng Liu,Miao Wu,Mengyin Peng

OncoTargets and therapy 13:4971-4985 PubMed32581556

2020

Identification of Aurora Kinase A as a Biomarker for Prognosis in Obesity Patients with Early Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Junhan Jiang,Zihe Guo,Junnan Xu,Tao Sun,Xinyu Zheng

Nature communications 11:2768 PubMed32488016

2020

Cellular census of human fibrosis defines functionally distinct stromal cell types and states.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas B Layton,Lynn Williams,Fiona McCann,Mingjun Zhang,Marco Fritzsche,Huw Colin-York,Marisa Cabrita,Michael T H Ng,Marc Feldmann,Stephen N Sansom,Dominic Furniss,Weilin Xie,Jagdeep Nanchahal

Thoracic cancer 11:1613-1620 PubMed32291953

2020

MicroRNA-125b as a tumor suppressor by targeting MMP11 in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yanan Wang,Yaning Wei,Xiangyu Fan,Pei Zhang,Pan Wang,Shujie Cheng,Jinku Zhang

Cell cycle (Georgetown, Tex.) 18:1560-1572 PubMed31157588

2019

Circular RNA 0047905 acts as a sponge for microRNA4516 and microRNA1227-5p, initiating gastric cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiyong Lai,Yang Yang,Chaobing Wang,Wenhui Yang,Yichao Yan,Zhu Wang,Jun Xu,Kewei Jiang

The international journal of biochemistry & cell b 102:1-9 PubMed29890225

2018

Mixomics analysis of breast cancer: Long non-coding RNA linc01561 acts as ceRNA involved in the progression of breast cancer.

Applications

Species

Unspecified reactive species

Rui Jiang,Chunming Zhao,Binbin Gao,Jiawen Xu,Wei Song,Peng Shi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MMP11 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP11 antibody (AB53143)

Western blot - Anti-MMP11 antibody (AB53143)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Complementary Products

Secondary Antibodies

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Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

Product promise