Anti-MMP11 antibody [EPR29490-537R]

• WB

Lab

Western blot - Anti-MMP11 antibody [EPR29490-537R] (AB326424)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : HT-29, HL-60.

The molecular weight observed is consistent with what has been described in the literature (PMID : 22227581; PMID : 12006591).

Bands observed at around 250 kDa may represent oligomeric forms of MMP11.

The identity of the lower MW band at approximately 20 kDa in lane 6 is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-MMP11 antibody [EPR29490-537R] (ab326424) at 1/1000 dilution

Lane 1:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 48 µg

Lane 2:

HepG2 culture supernatant (ultrafiltration) at 48 µg

Lane 3:

TT (human thyroid carcinoma epithelial cell) whole cell lysate at 48 µg

Lane 4:

TT culture supernatant (ultrafiltration) at 48 µg

Lane 5:

HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 48 µg

Lane 6:

HT-29 culture supernatant (ultrafiltration) at 48 µg

Lane 7:

HL-60 (human acute promyelocytic leukemia promyeloblast) whole cell lysate at 48 µg

Lane 8:

HL-60 culture supernatant (ultrafiltration) at 48 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 55 kDa,44 kDa,37 kDa,36 kDa

false

Exposure time: 180s