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AB137443

Anti-MMP12 antibody

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(4 Publications)

Rabbit Polyclonal MMP12 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human MMP12 aa 200-350.

View Alternative Names

HME, MMP12, Macrophage metalloelastase, MME, Macrophage elastase, Matrix metalloproteinase-12, ME, hME, MMP-12

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP12 antibody (AB137443)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP12 antibody (AB137443)

Immunohistochemical analysis of parafin-embedded DLD1 xenograft tissue labelling MMP12 with ab137443 at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-MMP12 antibody (AB137443)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MMP12 antibody (AB137443)

Immunofluorescence analysis of methanol-fixed H1299 cells labelling MMP12 with ab137443 at 1/500 dilution. The image in the lower panel is costained with Hoechst 33342.

Western blot - Anti-MMP12 antibody (AB137443)
  • WB

Supplier Data

Western blot - Anti-MMP12 antibody (AB137443)

Samples were separated by 10% SDS PAGE.

All lanes:

Western blot - Anti-MMP12 antibody (ab137443) at 1/1000 dilution

All lanes:

293T Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 54 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human MMP12 aa 200-350. The exact immunogen used to generate this antibody is proprietary information.

P39900

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Alternative Products

Assay Kits

AB255715

Human MMP12 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human MMP12 ELISA Kit (AB255715)

  • 0
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Primary Antibodies

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Anti-MMP12 antibody [EP1261Y] - BSA and Azide free

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Primary Antibodies

AB137444

Anti-MMP12 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP12 antibody (AB137444)

  • 4
  • 1
Primary Antibodies

AB52897

Anti-MMP12 antibody [EP1261Y]

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Immunoprecipitation - Anti-MMP12 antibody [EP1261Y] (AB52897)

  • 0
  • 0

Complementary Products

Assay Kits

AB246540

Mouse MMP12 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse MMP12 ELISA Kit (AB246540)

  • 0
  • 0
Primary Antibodies

AB76003

Anti-MMP9 antibody [EP1254]

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Secondary Antibodies

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB92536

Anti-MMP2 antibody [EPR1184]

RabMAb|Recombinant

Western blot - Anti-MMP2 antibody [EPR1184] (AB92536)

  • 4
  • 6
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Matrix metallopeptidase 12 commonly known as MMP12 or macrophage elastase is an enzyme with a molecular mass of approximately 54 kDa. Found mainly in macrophages MMP12 belongs to the matrix metallopeptidase family. It functions to break down extracellular matrix proteins specifically elastin. Besides macrophages it sees expression in other tissues including the lungs and skin but it holds particular importance in immune cell-related environments.
Biological function summary

MMP12 plays an important role in tissue remodeling and repair processes. It interacts with other proteases within the extracellular matrix. The degradation of elastin by MMP12 contributes to structural organization and it participates in immune response modulation. As part of the matrix metalloproteinase family MMP12 takes part in the larger proteolytic system which comprises multiple enzymes working in concert.

Pathways

MMP12 is an essential factor in the extracellular matrix degradation pathway. This pathway regulates tissue remodeling and inflammatory response. MMP12 independently breaks down elastin while it interacts with proteins like MMP2 and MMP9 which assist in remodeling processes. It also contributes to the regulation of cytokines that orchestrate immune functions.

Overexpression of MMP12 is strongly linked to chronic obstructive pulmonary disease (COPD) and atherosclerosis. In COPD MMP12's breakdown of elastin contributes to tissue damage in the lung. In atherosclerosis the enzyme accelerates plaque destabilization impacting cardiovascular health. Additionally MMP12 shares functional relationships with proteins such as TIMPs (tissue inhibitors of metalloproteinases) which regulate its activity and play roles in disease pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
See full target information MMP12
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:844 PubMed39557851

2024

RON receptor tyrosine kinase as a critical determinant in promoting tumorigenic behaviors of bladder cancer cells through regulating MMP12 and HIF-2α pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Ke-Jie Wang,Sha-Zhou Ye,Xiao-Long Jia,Kai-Yun Wang,Xiang-Yu Meng,Xin Fei,Shi-Jie Ye,Qi Ma

Biomolecules 13: PubMed37189419

2023

Matrix Metalloproteinases Contribute to the Calcification Phenotype in Pseudoxanthoma Elasticum.

Applications

Unspecified application

Species

Unspecified reactive species

Ricarda Plümers,Christopher Lindenkamp,Michel Robin Osterhage,Cornelius Knabbe,Doris Hendig

Atherosclerosis 355:8-14 PubMed35850021

2022

Increased proteolytic cleavage of osteoglycin is associated with a stable plaque phenotype and lower risk of cardiovascular events.

Applications

Unspecified application

Species

Unspecified reactive species

Dania Al-Sharify,Signe Holm Nielsen,Frank Matthes,Christoffer Tengryd,Jiangming Sun,Federica Genovese,Morten A Karsdal,Jan Nilsson,Isabel Goncalves,Andreas Edsfeldt

The Journal of biological chemistry 291:26816-26836 PubMed27799301

2016

Anoctamin 6 Contributes to Cl- Secretion in Accessory Cholera Enterotoxin (Ace)-stimulated Diarrhea: AN ESSENTIAL ROLE FOR PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE (PIP2) SIGNALING IN CHOLERA.

Applications

Unspecified application

Species

Unspecified reactive species

Joydeep Aoun,Mikio Hayashi,Irshad Ali Sheikh,Paramita Sarkar,Tultul Saha,Priyanka Ghosh,Rajsekhar Bhowmick,Dipanjan Ghosh,Tanaya Chatterjee,Pinak Chakrabarti,Manoj K Chakrabarti,Kazi Mirajul Hoque
View all publications
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Product promise

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