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AB231096

Anti-MMP12 antibody

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(1 Publication)

Rabbit Polyclonal MMP12 antibody. Suitable for WB and reacts with Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Rat Mmp12 aa 100-300.

View Alternative Names

Mmel, Mmp12, Macrophage metalloelastase, MME, Matrix metalloproteinase-12, MMP-12

5 Images
Western blot - Anti-MMP12 antibody (AB231096)
  • WB

Supplier Data

Western blot - Anti-MMP12 antibody (AB231096)

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Western blot - Anti-MMP12 antibody (ab231096) at 1 µg/mL

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Rat liver lysate

Predicted band size: 54 kDa

false

Western blot - Anti-MMP12 antibody (AB231096)
  • WB

Supplier Data

Western blot - Anti-MMP12 antibody (AB231096)

All lanes:

Western blot - Anti-MMP12 antibody (ab231096) at 2 µg/mL

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Rat small intestine lysate

Predicted band size: 54 kDa

false

Western blot - Anti-MMP12 antibody (AB231096)
  • WB

Supplier Data

Western blot - Anti-MMP12 antibody (AB231096)

All lanes:

Western blot - Anti-MMP12 antibody (ab231096) at 2 µg/mL

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Rat thymus lysate

Predicted band size: 54 kDa

false

Western blot - Anti-MMP12 antibody (AB231096)
  • WB

Supplier Data

Western blot - Anti-MMP12 antibody (AB231096)

All lanes:

Western blot - Anti-MMP12 antibody (ab231096) at 2 µg/mL

All lanes:

Rat spinal cord lysate

Predicted band size: 54 kDa

false

Western blot - Anti-MMP12 antibody (AB231096)
  • WB

Supplier Data

Western blot - Anti-MMP12 antibody (AB231096)

All lanes:

Western blot - Anti-MMP12 antibody (ab231096) at 2 µg/mL

All lanes:

Recombinant rat MMP12 protein

Predicted band size: 54 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Rat Mmp12 aa 100-300. The exact immunogen used to generate this antibody is proprietary information.

Q63341

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab231096 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Matrix metallopeptidase 12 commonly known as MMP12 or macrophage elastase is an enzyme with a molecular mass of approximately 54 kDa. Found mainly in macrophages MMP12 belongs to the matrix metallopeptidase family. It functions to break down extracellular matrix proteins specifically elastin. Besides macrophages it sees expression in other tissues including the lungs and skin but it holds particular importance in immune cell-related environments.
Biological function summary

MMP12 plays an important role in tissue remodeling and repair processes. It interacts with other proteases within the extracellular matrix. The degradation of elastin by MMP12 contributes to structural organization and it participates in immune response modulation. As part of the matrix metalloproteinase family MMP12 takes part in the larger proteolytic system which comprises multiple enzymes working in concert.

Pathways

MMP12 is an essential factor in the extracellular matrix degradation pathway. This pathway regulates tissue remodeling and inflammatory response. MMP12 independently breaks down elastin while it interacts with proteins like MMP2 and MMP9 which assist in remodeling processes. It also contributes to the regulation of cytokines that orchestrate immune functions.

Overexpression of MMP12 is strongly linked to chronic obstructive pulmonary disease (COPD) and atherosclerosis. In COPD MMP12's breakdown of elastin contributes to tissue damage in the lung. In atherosclerosis the enzyme accelerates plaque destabilization impacting cardiovascular health. Additionally MMP12 shares functional relationships with proteins such as TIMPs (tissue inhibitors of metalloproteinases) which regulate its activity and play roles in disease pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3 (By similarity).
See full target information Mmp12
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:3517 PubMed38347040

2024

Aqueous humor TGFβ and fibrillin-1 in Tsk mice reveal clues to POAG pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

James C Tan,MinHee K Ko,Jeong-Im Woo,Kenneth L Lu,Jonathan A Kelber
View all publications
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Product promise

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