AB231109

Anti-MMP12 antibody

Name: Anti-MMP12 antibody (ab231109) | Abcam
Brand: Abcam Limited
SKU: AB231109
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal MMP12 antibody. Suitable for WB and reacts with Rat, Recombinant full length protein - Rat, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Rat Mmp12 aa 350 to C-terminus.

View Alternative Names

Mme, Mmel, Mmp12, Macrophage metalloelastase, MME, Matrix metalloproteinase-12, MMP-12

3 Images

Supplier Data

Western blot - Anti-MMP12 antibody (AB231109)

All lanes:

Western blot - Anti-MMP12 antibody (ab231109) at 2 µg/mL

All lanes:

Rat cerebrum lysate

Predicted band size: 54 kDa

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Supplier Data

Western blot - Anti-MMP12 antibody (AB231109)

All lanes:

Western blot - Anti-MMP12 antibody (ab231109) at 2 µg/mL

All lanes:

Mouse cerebrum lysate

Predicted band size: 54 kDa

true

Supplier Data

Western blot - Anti-MMP12 antibody (AB231109)

All lanes:

Western blot - Anti-MMP12 antibody (ab231109) at 2 µg/mL

All lanes:

Recombinant rat MMP12

Predicted band size: 54 kDa,61 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

applications

Immunogen

Recombinant Fragment Protein within Rat Mmp12 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q63341

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "" }, "Recombinant full length protein - Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Matrix metallopeptidase 12 commonly known as MMP12 or macrophage elastase is an enzyme with a molecular mass of approximately 54 kDa. Found mainly in macrophages MMP12 belongs to the matrix metallopeptidase family. It functions to break down extracellular matrix proteins specifically elastin. Besides macrophages it sees expression in other tissues including the lungs and skin but it holds particular importance in immune cell-related environments.

Biological function summary

MMP12 plays an important role in tissue remodeling and repair processes. It interacts with other proteases within the extracellular matrix. The degradation of elastin by MMP12 contributes to structural organization and it participates in immune response modulation. As part of the matrix metalloproteinase family MMP12 takes part in the larger proteolytic system which comprises multiple enzymes working in concert.

Pathways

MMP12 is an essential factor in the extracellular matrix degradation pathway. This pathway regulates tissue remodeling and inflammatory response. MMP12 independently breaks down elastin while it interacts with proteins like MMP2 and MMP9 which assist in remodeling processes. It also contributes to the regulation of cytokines that orchestrate immune functions.

Overexpression of MMP12 is strongly linked to chronic obstructive pulmonary disease (COPD) and atherosclerosis. In COPD MMP12's breakdown of elastin contributes to tissue damage in the lung. In atherosclerosis the enzyme accelerates plaque destabilization impacting cardiovascular health. Additionally MMP12 shares functional relationships with proteins such as TIMPs (tissue inhibitors of metalloproteinases) which regulate its activity and play roles in disease pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3 (By similarity).

See full target information Mmp12

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Proteome science 19:9 PubMed34330296

2021

iTRAQ-based proteomic analysis of sperm reveals candidate proteins that affect the quality of spermatozoa from boars on plateaus.

Applications

Unspecified application

Species

Unspecified reactive species

Yanling Zhao,Yaomei Wang,Feipeng Guo,Bo Lu,Jiale Sun,Jianzhou Wang,Zili Ren

View all publications

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