AB52897

Anti-MMP12 antibody [EP1261Y]

RabMAb
Recombinant
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(52 Publications)

Rabbit Recombinant Monoclonal MMP12 antibody. Suitable for IP, WB and reacts with Human, Recombinant fragment samples. Cited in 52 publications.

View Alternative Names

HME, MMP12, Macrophage metalloelastase, MME, Macrophage elastase, Matrix metalloproteinase-12, ME, hME, MMP-12

3 Images

Unknown

Immunoprecipitation - Anti-MMP12 antibody [EP1261Y] (AB52897)

ab52897 (purified) at 1/20 immunoprecipitating Glutamate Receptor 1 in HeLa whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/1,500) was used for detection. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-MMP12 antibody [EP1261Y] (ab52897)

Predicted band size: 54 kDa

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Western blot - Anti-MMP12 antibody [EP1261Y] (AB52897)

Blocking buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-MMP12 antibody [EP1261Y] (ab52897) at 1/2000 dilution

Lane 1:

HT-1080 cell lysate at 10 µg

Lane 2:

WI-38 cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/1000 dilution

Predicted band size: 54 kDa

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Western blot - Anti-MMP12 antibody [EP1261Y] (AB52897)

Based on Western blot, unpurified ab52897 recognizes both pro and active MMP. The propeptide is 54 kDa, and 45 kDa and 22 kDa are active forms. The primary was used at 1 : 100 to detect recombinant MMP12 protein, but can be diluted more to decrease the intensity of the bands.

All lanes:

Western blot - Anti-MMP12 antibody [EP1261Y] (ab52897)

Predicted band size: 54 kDa

false

Carrier free

Anti-MMP12 antibody [EP1261Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1261Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Complementary Products

Assay Kits

AB255715

Human MMP12 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human MMP12 ELISA Kit (AB255715)

Primary Antibodies

AB76003

Anti-MMP9 antibody [EP1254]

RabMAb|Recombinant|KO Validated

Western blot - Anti-MMP9 antibody [EP1254] (AB76003)

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Primary Antibodies

AB92536

Anti-MMP2 antibody [EPR1184]

RabMAb|Recombinant

Western blot - Anti-MMP2 antibody [EPR1184] (AB92536)

Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/20", "IP-species-notes": "For unpurified use at 1/30.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/30", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Recombinant fragment": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Matrix metallopeptidase 12 commonly known as MMP12 or macrophage elastase is an enzyme with a molecular mass of approximately 54 kDa. Found mainly in macrophages MMP12 belongs to the matrix metallopeptidase family. It functions to break down extracellular matrix proteins specifically elastin. Besides macrophages it sees expression in other tissues including the lungs and skin but it holds particular importance in immune cell-related environments.

Biological function summary

MMP12 plays an important role in tissue remodeling and repair processes. It interacts with other proteases within the extracellular matrix. The degradation of elastin by MMP12 contributes to structural organization and it participates in immune response modulation. As part of the matrix metalloproteinase family MMP12 takes part in the larger proteolytic system which comprises multiple enzymes working in concert.

Pathways

MMP12 is an essential factor in the extracellular matrix degradation pathway. This pathway regulates tissue remodeling and inflammatory response. MMP12 independently breaks down elastin while it interacts with proteins like MMP2 and MMP9 which assist in remodeling processes. It also contributes to the regulation of cytokines that orchestrate immune functions.

Overexpression of MMP12 is strongly linked to chronic obstructive pulmonary disease (COPD) and atherosclerosis. In COPD MMP12's breakdown of elastin contributes to tissue damage in the lung. In atherosclerosis the enzyme accelerates plaque destabilization impacting cardiovascular health. Additionally MMP12 shares functional relationships with proteins such as TIMPs (tissue inhibitors of metalloproteinases) which regulate its activity and play roles in disease pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.

See full target information MMP12

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Publications (52)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in medicine 12:1529495 PubMed40421300

2025

Proteomic insights into COPD pathogenesis and therapeutic targets: a causal analysis of circulating proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Min Luo,Ying Tang,Jing Qiu,Kai Yang,WenJing Dai,EnRong Fang

Journal of Cancer 16:183-200 PubMed39744560

2025

Deciphering the Prognostic Landscape of Esophageal Adenocarcinoma: A PANoptosis-Related Gene Signature.

Applications

Unspecified application

Species

Unspecified reactive species

Haijing Fu,Mengyan Liu,Huiyu Li,Li Yu,Haizhu Song,Xiaoyuan Chu,Wei Bao

International journal of molecular sciences 25: PubMed38891996

2024

Pharmacological Inhibition of MMP-12 Exerts Protective Effects on Angiotensin II-Induced Abdominal Aortic Aneurysms in Apolipoprotein E-Deficient Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Karina Di Gregoli,Georgia Atkinson,Helen Williams,Sarah J George,Jason L Johnson

OncoTargets and therapy 17:113-128 PubMed38384996

2024

Exosome-Delivered EGFR Induced by Acidic Bile Salts Regulates Macrophage M2 Polarization to Promote Esophageal Adenocarcinoma Cell Proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Chuangui Chen,Jinsheng Ding,Zhao Ma,Yongjie Xie,Linhua Zhang,Dunwan Zhu

International journal of chronic obstructive pulmonary disease 19:269-279 PubMed38288346

2024

MTOR Suppresses Cigarette Smoke-Induced Airway Inflammation and MMP12 Expression in Macrophage in Chronic Obstructive Pulmonary Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Lingling Dong,Yong Wang,Haipin Chen,Zhouyang Li,Xuchen Xu,Jiesen Zhou,Huahao Shen,Zhihua Chen

Journal of the American Heart Association 12:e028215 PubMed36565196

2022

Proteolytic Degradation Is a Major Contributor to Bioprosthetic Heart Valve Failure.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander E Kostyunin,Tatiana V Glushkova,Arseniy A Lobov,Evgeny A Ovcharenko,Bozhana R Zainullina,Leo A Bogdanov,Daria K Shishkova,Victoria E Markova,Maksim A Asanov,Rinat A Mukhamadiyarov,Elena A Velikanova,Tatiana N Akentyeva,Maria A Rezvova,Alexander N Stasev,Alexey V Evtushenko,Leonid S Barbarash,Anton G Kutikhin

Molecular medicine reports 26: PubMed35894144

2022

Interference of PTK6/GAB1 signaling inhibits cell proliferation, invasion, and migration of cervical cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Li,Nan Yang,Xiaolei Tian,Linglong Ouyang,Man Jiang,Shufang Zhang

Experimental and therapeutic medicine 24:549 PubMed35978915

2022

Ropivacaine has the potential to relieve PM2.5-induced acute lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Zuo,Xin-Yu Li,Yong-Guan He

Bioengineered 13:11945-11957 PubMed35549637

2022

Knockdown of KRAB domain-associated protein 1 suppresses the proliferation, migration and invasion of thyroid cancer cells by regulating P68/DEAD box protein 5.

Applications

Unspecified application

Species

Unspecified reactive species

Hai Lan,Congyao Lin,Hongyin Yuan

Bioengineered 13:12115-12126 PubMed35546072

2022

Far upstream element -binding protein 1 (FUBP1) participates in the malignant process and glycolysis of colon cancer cells by combining with c-Myc.

Applications

Unspecified application

Species

Unspecified reactive species

Shanwei Wang,Yanli Wang,Sheng Li,Shen Nian,Wenjing Xu,Fenli Liang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MMP12 antibody [EP1261Y]

Immunoprecipitation - Anti-MMP12 antibody [EP1261Y] (AB52897)

Western blot - Anti-MMP12 antibody [EP1261Y] (AB52897)

Western blot - Anti-MMP12 antibody [EP1261Y] (AB52897)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (52)

Product promise