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AB231217

Anti-MMP13 antibody

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(3 Publications)

Guinea pig Polyclonal MMP13 antibody. Suitable for WB and reacts with Recombinant fragment - Rabbit, Rabbit samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Rabbit MMP13 aa 50-250.

View Alternative Names

Collagenase 3, Matrix metalloproteinase-13, MMP-13, MMP13

2 Images
Western blot - Anti-MMP13 antibody (AB231217)
  • WB

Supplier Data

Western blot - Anti-MMP13 antibody (AB231217)

All lanes:

Western blot - Anti-MMP13 antibody (ab231217) at 2 µg/mL

All lanes:

Recombinant rabbit MMP13 protein

Predicted band size: 54 kDa

false

Western blot - Anti-MMP13 antibody (AB231217)
  • WB

Supplier Data

Western blot - Anti-MMP13 antibody (AB231217)

All lanes:

Western blot - Anti-MMP13 antibody (ab231217) at 5 µg/mL

All lanes:

Rabbit serum

Predicted band size: 54 kDa

false

Key facts

Host species

Guinea pig

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rabbit

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Rabbit MMP13 aa 50-250. The exact immunogen used to generate this antibody is proprietary information.

O62806

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  • 0
  • 0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>" }, "Recombinant fragment - Rabbit": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Storage buffer
pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Matrix metallopeptidase 13 (MMP-13) also called collagenase 3 is an enzyme with an approximate molecular mass of 54 kDa. It plays a significant role in the breakdown of extracellular matrix components particularly collagen. MMP-13 is expressed in several tissues including cartilage skin and bone. Its expression sees an increase during tissue remodeling and in pathological conditions. MMP-13 also participates in processes like wound healing and embryonic development making it a focal point for understanding tissue dynamics.
Biological function summary

Matrix metallopeptidase 13 contributes extensively to the degradation of collagen type II a major component of cartilage. As a zinc-dependent endopeptidase MMP-13 is part of the MMP family which facilitates the remodeling of the extracellular matrix. MMP-13 is involved in the proteolytic cascade working in conjunction with other MMPs and elastase to mediate tissue repair and turnover. It does not form complexes but acts in concert with other enzymes to execute its physiological functions effectively.

Pathways

Matrix metallopeptidase 13 significance is most noted in the cartilage degradation pathway. It interacts with other MMPs such as MMP-1 and MMP-9 to efficiently break down extracellular matrix components. These interactions highlight its role in the regulation of matrix metalloproteinase activity within the connective tissue degradation pathway. These pathways are key during normal connective tissue remodeling and in pathological processes illustrating the essential roles of MMPs in maintaining tissue homeostasis.

Matrix metallopeptidase 13 has strong associations with osteoarthritis and rheumatoid arthritis. It contributes to cartilage destruction intensifying the progression of these disorders due to its potent collagenolytic activity. Other proteins like MMP-9 and MMP-14 are also involved in these processes potentially amplifying tissue damage in affected joints. Understanding how MMP-13 and related proteins drive these diseases offers potential therapeutic targets for slowing disease progression and enhancing joint health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CCN2. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CCN2. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion (By similarity).
See full target information MMP13
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 10:e39543 PubMed39498075

2024

Targeted radionuclide therapy against GARP expressing T regulatory cells after tumour priming with external beam radiotherapy in a murine syngeneic model.

Applications

Unspecified application

Species

Unspecified reactive species

Pierre-Simon Bellaye,Alexandre Mm Dias,Jean-Marc Vrigneaud,Alexanne Bouchard,Mathieu Moreau,Camille Petitot,Claire Bernhard,Michael Claron,Lisa Froidurot,Véronique Morgand,Mélanie Guillemin,Marie Monterrat,Céline Mirjolet,Carmen Garrido,Evelyne Kohli,Bertrand Collin

Journal of orthopaedic translation 33:90-106 PubMed35330941

2022

3D printed porous sulfonated polyetheretherketone scaffold for cartilage repair: Potential and limitation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiguo Yuan,Teng Long,Jue Zhang,Zhuocheng Lyu,Wei Zhang,Xiangchao Meng,Jin Qi,You Wang

Journal of nanobiotechnology 19:194 PubMed34193158

2021

Mesenchymal stem cell-derived extracellular vesicles prevent the development of osteoarthritis via the circHIPK3/miR-124-3p/MYH9 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Shenglong Li,Jie Liu,Siyu Liu,Weijie Jiao,Xiaohong Wang
View all publications
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