Anti-MMP13 antibody is a rabbit polyclonal antibody that is used to detect MMP13 in IHC, western blot, ICC/IF, ELISA and flow cytometry. Suitable for human, mouse, rat, and more samples.
- Cited in over 515 publications
- One antibody for all your MMP13 staining
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Publications
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- Scientific reports 13:213492023A causative role for periarticular skeletal muscle weakness in the progression of joint damage and pain in OA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJu-Ryoung Kim,Thi Hong Nhung Pham,Wan-Uk Kim,Hyun Ah KimPubMed 38049482
- Marine drugs 21:2023Low-Molecular-Weight Fish Collagen Peptide (Valine-Glycine-Proline-Hydroxyproline-Glycine-Proline-Alanine-Glycine) Prevents Osteoarthritis Symptoms in Chondrocytes and Monoiodoacetate-Injected Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWonhee Cho,Jeongjin Park,Jinhee Kim,Minhee Lee,So Jung Park,Kyung Seok Kim,Woojin Jun,Ok-Kyung Kim,Jeongmin LeePubMed 38132929
- Frontiers in pharmacology 14:12502162023IL-37 counteracts inflammatory injury in the temporomandibular joint via the intracellular pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Li,Sisi Peng,Ying Yan,Shan Yan,Xin Cao,Yong Li,Luying Zhu,Jie XuPubMed 38053836
- International journal of molecular sciences 24:2023Enhanced Intervertebral Disc Repair via Genetically Engineered Mesenchymal Stem Cells with Tetracycline Regulatory System.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYeji Kim,Seong Bae An,Sang-Hyuk Lee,Jong Joo Lee,Sung Bum Kim,Jae-Cheul Ahn,Dong-Youn Hwang,Inbo HanPubMed 38003216
- Biomolecules 13:2023Early Resveratrol Treatment Mitigates Joint Degeneration and Dampens Pain in a Mouse Model of Pseudoachondroplasia (PSACH).Applications:Unspecified applicationReactive species:Unspecified reactive speciesJacqueline T Hecht,Alka C Veerisetty,Debabrata Patra,Mohammad G Hossain,Frankie Chiu,Claire Mobed,Francis H Gannon,Karen L PoseyPubMed 37892235
- Journal of orthopaedic surgery and research 18:7842023PLXNC1 interference alleviates the inflammatory injury, apoptosis and extracellular matrix degradation of IL-1β-exposed chondrocytes via suppressing GRP78 expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNan Meng,Lingwei Mao,Qinyi Jiang,Jishan Yuan,Linjuan Liu,Lei WangPubMed 37853395
- iScience 26:1081192023Mechanical overloading leads to chondrocyte degeneration and senescence via Zmpste24-mediated nuclear membrane instability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKeyu Kong,Minghao Jin,Chen Zhao,Hua Qiao,Xuzhuo Chen,Baixing Li,Kewei Rong,Pu Zhang,Yu Shan,Zhengquan Xu,Yongyun Chang,Huiwu Li,Zanjing ZhaiPubMed 37965144
- Gerontology 69:1295-13062023Age-Related Gene and Protein Expression in Mouse Mandibular Condyle Analyzed by Cap Analysis of Gene Expression and Immunohistochemistry.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMu-Chen Yang,Megumi Nakamura,Yoko Kageyama,Yohei Igari,Yasuyuki SasanoPubMed 37769633
- Clinical and translational medicine 13:e14152023Circular RNA circARPC1B functions as a stabilisation enhancer of Vimentin to prevent high cholesterol-induced articular cartilage degeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiarui Li,Xiang Li,Shengji Zhou,Yuxin Wang,Tiantian Ying,Quan Wang,Yizheng Wu,Fengchao ZhaoPubMed 37740460
- Arthritis research & therapy 25:1682023Effects of low-intensity exercise on spontaneously developed knee osteoarthritis in male senescence-accelerated mouse prone 8.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKosuke Norimatsu,Kazuki Nakanishi,Toshiro Ijuin,Shotaro Otsuka,Seiya Takada,Akira Tani,Ryoma Matsuzaki,Teruki Matsuoka,Harutoshi SakakimaPubMed 37710278
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human MMP13. The exact immunogen used to generate this antibody is proprietary information. Database link P45452
Reactivity data
Select an application| Flow Cyt | WB | sELISA | IHC-P | ICC/IF | IHC-Fr | |
|---|---|---|---|---|---|---|
| Human | Expected | Tested | Expected | Expected | Expected | Expected |
| Mouse | Expected | Predicted | Predicted | Expected | Expected | Expected |
| Rat | Expected | Predicted | Predicted | Expected | Expected | Expected |
| Dog | Expected | Predicted | Predicted | Expected | Expected | Expected |
| Synthetic peptide - Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
Flow Cyt
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Rat | Dilution info - | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Human | Dilution info - | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Dog | Dilution info - | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/3000.00000 - 1/6000.00000 | Notes 1/3000, when using colorimetric substrates such as BCIP/NBT - 1/6000, when using chemiluminescent substrates. Dilution optimised using Chromogenic detection. When used against the reduced protein identifies a band at 60 Kd. Note: The low endogenous protein levels may require concentration of samples by ultrafiltration or ammonium sulfate precipitation prior to Western blotting. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Dog, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes For sandwich ELISA, use this antibody as Detection at 0.5μg/ml with ab77949 as Capture. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Dog, Rat | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Dog | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Dog | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
IHC-Fr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Dog | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
Associated Products
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Target data
Function
Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CCN2. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CCN2. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.
Alternative names
Collagenase 3, Matrix metalloproteinase-13, MMP-13, MMP13
Recommended products
Anti-MMP13 antibody is a rabbit polyclonal antibody that is used to detect MMP13 in IHC, western blot, ICC/IF, ELISA and flow cytometry. Suitable for human, mouse, rat, and more samples.
- Cited in over 515 publications
- One antibody for all your MMP13 staining
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human MMP13. The exact immunogen used to generate this antibody is proprietary information. Database link P45452
- Purification technique
- Affinity purification Immunogen
- Specificity
ab39012 recognizes the latent proenzyme, at 60 Kd, as well as the active form at 48 Kd, and intermediate activation forms. It does not cross react with the other MMP family members.ab39012 recognizes the Hinge region of MMP13.
- Concentration
- Purification notes
The antibody has been peptide-affinity purified.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-MMP13 antibody (ab39012) is a rabbit polyclonal antibody and is validated for use in Flow Cyt, ICC/IF, IHC-Fr, IHC-P, WB, sELISA in dog, human, mouse, rat samples.
Anti-MMP13 antibody (ab39012) specifically detects MMP13 (UniProt ID: P45452; Molecular weight: 42kDa) and is sold in 100 µg selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-MMP13 antibody (ab39012) has high sensitivity and specificity.
Anti-MMP13 antibody (ab39012) has been cited over 517 times in peer reviewed journals and is trusted by the scientific community.
Anti-MMP13 antibody (ab39012) has 18 independent reviews from customers.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-MMP13 antibody (ab39012)
The loading amount is 15 ul/lane, and the condrosarcoma media was concentrated 40x.
All lanes: Western blot - Anti-MMP13 antibody (ab39012) at 1/3000 dilution
Lane 1: cell media from human chondrosarcoma (untreated).
Lane 2: cell media from human chondrosarcoma (IL1 beta treated).
Predicted band size: 54 kDa
Observed band size: 60 kDa
Sandwich ELISA - Anti-MMP13 antibody (ab39012)
Standard curve for MMP13; dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal [181-15A12] to MMP13 (ab77949) at 1μg/ml and Detector Antibody Rabbit polyclonal to MMP13 - Hinge region (ab39012) at 0.5μg/ml.
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