AB219620

Anti-MMP13 antibody [EPR21778]

Name: Anti-MMP13 antibody [EPR21778] (ab219620) | Abcam
Brand: Abcam Limited
SKU: AB219620
Price: 330 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(71 Publications)

Anti-MMP13 antibody [EPR21778] (ab219620) is a rabbit monoclonal antibody detecting MMP13 in IHC-P. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 30 publications

View Alternative Names

Collagenase 3, Matrix metalloproteinase-13, MMP-13, MMP13

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [EPR21778] (AB219620)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [EPR21778] (AB219620)

Immunohistochemical analysis of paraffin-embedded human tibia tissue labeling MMP13 with ab219620 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on cortical bone of human tibia (PMID : 22549931) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [EPR21778] (AB219620)

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling MMP13 with ab219620 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Counter stained with hematoxylin.

Negative control : No staining on human skeletal muscle (PMID : 9056642) is observed.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [EPR21778] (AB219620)

Immunohistochemical analysis of paraffin-embedded rat femur tissue labeling MMP13 with ab219620 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on cortical bone of rat femur (PMID : 22549931) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [EPR21778] (AB219620)

Immunohistochemical analysis of paraffin-embedded mouse femur tissue labeling MMP13 with ab219620 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on cortical bone of mouse femur (PMID : 22549931) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Carrier free

Anti-MMP13 antibody [EPR21778] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21778

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab219620 showed specific staining in cortical bone, but that no staining was observed in the breast cancer tissue tested.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "For human. Use at 1/500 dilution for mouse and rat.Recommend to incubate the primary antibody at 4C overnight and use the HRP-polymer secondary antibody to detect." }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "For human. Use at 1/500 dilution for mouse and rat.Recommend to incubate the primary antibody at 4C overnight and use the HRP-polymer secondary antibody to detect." }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "For human. Use at 1/500 dilution for mouse and rat.Recommend to incubate the primary antibody at 4C overnight and use the HRP-polymer secondary antibody to detect." } } }

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Product details

What is this antibody validated in?
Anti-MMP13 antibody [EPR21778] (ab219620) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples.

Trusted by the scientific community
Anti-MMP13 [EPR21778] (ab219620) was first used in a scientific publication in 2018 and has been cited over 30 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR21778] also available for your convenience: ab219620, Carrier free - ab237604

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Matrix metallopeptidase 13 (MMP-13) also called collagenase 3 is an enzyme with an approximate molecular mass of 54 kDa. It plays a significant role in the breakdown of extracellular matrix components particularly collagen. MMP-13 is expressed in several tissues including cartilage skin and bone. Its expression sees an increase during tissue remodeling and in pathological conditions. MMP-13 also participates in processes like wound healing and embryonic development making it a focal point for understanding tissue dynamics.

Biological function summary

Matrix metallopeptidase 13 contributes extensively to the degradation of collagen type II a major component of cartilage. As a zinc-dependent endopeptidase MMP-13 is part of the MMP family which facilitates the remodeling of the extracellular matrix. MMP-13 is involved in the proteolytic cascade working in conjunction with other MMPs and elastase to mediate tissue repair and turnover. It does not form complexes but acts in concert with other enzymes to execute its physiological functions effectively.

Pathways

Matrix metallopeptidase 13 significance is most noted in the cartilage degradation pathway. It interacts with other MMPs such as MMP-1 and MMP-9 to efficiently break down extracellular matrix components. These interactions highlight its role in the regulation of matrix metalloproteinase activity within the connective tissue degradation pathway. These pathways are key during normal connective tissue remodeling and in pathological processes illustrating the essential roles of MMPs in maintaining tissue homeostasis.

Matrix metallopeptidase 13 has strong associations with osteoarthritis and rheumatoid arthritis. It contributes to cartilage destruction intensifying the progression of these disorders due to its potent collagenolytic activity. Other proteins like MMP-9 and MMP-14 are also involved in these processes potentially amplifying tissue damage in affected joints. Understanding how MMP-13 and related proteins drive these diseases offers potential therapeutic targets for slowing disease progression and enhancing joint health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CCN2. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CCN2. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.

See full target information MMP13

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Publications (71)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113097 PubMed40822351

2025

SESN2 maintains cartilage homeostasis by SREBP1-mediated lipid metabolism during osteoarthritis progression.

Applications

Unspecified application

Species

Unspecified reactive species

Zizheng Liu,Huiming Jiang,Zhaofeng Zhang,Ruiyang Jiang,Rongliang Wang,Jiawei Li,Weitong Li,Guihua Tan,Yuxiang Fei,Zhongyang Lv,Hu Guo,Ziyin Sun,Rui Wu,Hongfei Shi,Dongquan Shi

Drug design, development and therapy 19:5855-5870 PubMed40657039

2025

Vitamin D Alleviates Osteoarthritis Progression by Targeting Cartilage and Subchondral Bone via Myd88-TAK1-ERK Axis Suppression.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Gao,Yalin Min,Rui Lin,Dahong Liang,Min Zhang,Qixian Xiao,Yan Lu,Fucheng Zhang,Bilian Xu,Yanzhi Liu

Frontiers in bioengineering and biotechnology 13:1555983 PubMed40599407

2025

Distribution and metabolism of iPSC-MSCs in the joint cavity of an osteoarthritis rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyang Yuan,Xulei Wang,Tianxi Du,Feng Zhang,Gang Cheng,Kang Wang,Haochen Xu,Pingting Yang,Yan Chang,Wei Wei,Peng He,Shangxue Yan

Materials today. Bio 33:101944 PubMed40585025

2025

A microsphere loaded with chondrocyte-targeting exosomes continuously deliver miR-148a for osteoarthritis therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Yang,Haiwei Ma,Kechi Li,Jie Xiang,Jianxin Qiu,Yiming Ding,Chengdi Wang,Hehuan Lai,Xingyu Hu,Shijie Liu,Chao Lou,Zhenhua Hong,Zhenzhong Chen,Dengwei He

Journal of nanobiotechnology 23:389 PubMed40426246

2025

Extracellular vesicles from adipose-derived stromal/stem cells reprogram dendritic cells to alleviate rat TMJOA by transferring mitochondria.

Applications

Unspecified application

Species

Unspecified reactive species

Ziyi Mei,Hanyue Li,Chuling Huang,Shiyong Ma,Yuejia Li,Pingmeng Deng,Sha Zhou,Aizhuo Qian,Bin Yang,Jie Li

Journal of inflammation research 18:5293-5309 PubMed40264591

2025

Gubi Decoction Ameliorates Porous Cartilage Endplate in an Intervertebral Disc Degeneration Model Mouse Through Inhibition of NF-κB Activity and Pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Sai Yao,Yanan Li,Hongfeng Ruan,Lianguo Wu,Hanbing Zeng

Journal of orthopaedic translation 51:132-144 PubMed40129610

2025

JP4-039 protects chondrocytes from ferroptosis to attenuate osteoarthritis progression by promoting Pink1/Parkin-dependent mitophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Ya Xie,Zhongyang Lv,Weitong Li,JinTao Lin,Wei Sun,Hu Guo,Xiaoyu Jin,Yuan Liu,Ruiyang Jiang,Yuxiang Fei,Rui Wu,Dongquan Shi

Journal of nanobiotechnology 23:175 PubMed40050923

2025

Regulating macrophage phenotypes with IL4I1-mimetic nanoparticles in IDD treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaying Luo,Guoxin Jin,Shaoqian Cui,Huan Wang,Qi Liu

Experimental biology and medicine (Maywood, N.J.) 249:10066 PubMed39911634

2025

Impaired fracture healing is associated with callus chondro-osseous junction abnormalities in periostin-null and osteopontin-null mice.

Applications

Unspecified application

Species

Unspecified reactive species

Marc Teitelbaum,Maya D Culbertson,Charlene Wetterstrand,J Patrick O'Connor

Organogenesis 21:2460269 PubMed39905673

2025

The Role of SUMO1 Modification of SOX9 in Cartilage Development Stimulated by Zinc Ions in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Na Xue,Jing Zhao,Jing Yin,Liang Liu,Zhong Yang,Shuchao Zhai,Xiyun Bian,Xiang Gao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MMP13 antibody [EPR21778]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [EPR21778] (AB219620)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [EPR21778] (AB219620)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [EPR21778] (AB219620)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [EPR21778] (AB219620)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (71)

Product promise