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Rabbit Recombinant Monoclonal MMP13 antibody. Carrier free. Suitable for IHC-P and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [EPR21778] - BSA and Azide free (AB237604), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [EPR21778] - BSA and Azide free (AB237604), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [EPR21778] - BSA and Azide free (AB237604), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [EPR21778] - BSA and Azide free (AB237604), expandable thumbnail

Publications

  • International journal of molecular sciences 24:2023
    Chondrocyte Thrombomodulin Protects against Osteoarthritis.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Lin Kang,Ai-Lun Yang,Chao-Han Lai,Tsan-Ju Chen,Sung-Yen Lin,Yan-Hsiung Wang,Chau-Zen Wang,Edward M Conway,Hua-Lin Wu,Mei-Ling Ho,Je-Ken Chang,Chung-Hwan Chen,Tsung-Lin Cheng
    PubMed 37298473
  • Cell death & disease 12:8862021
    Inhibition of nuclear receptor RORα attenuates cartilage damage in osteoarthritis by modulating IL-6/STAT3 pathway.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Tongzhou Liang,Taiqiu Chen,Jincheng Qiu,Wenjie Gao,Xianjian Qiu,Yuanxin Zhu,Xudong Wang,Yanbo Chen,Hang Zhou,Zhihuai Deng,Pengfei Li,Caixia Xu,Yan Peng,Anjing Liang,Peiqiang Su,Bo Gao,Dongsheng Huang
    PubMed 34584074

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Human
Tested
Mouse
Tested
Rat
Tested

Tested
Tested

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

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    1 products for Alternative Version

      Target data

      Function

      Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CCN2. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CCN2. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.

      Alternative names

      Collagenase 3, Matrix metalloproteinase-13, MMP-13, MMP13

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      Rabbit Recombinant Monoclonal MMP13 antibody. Carrier free. Suitable for IHC-P and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

      Alternative names

      Collagenase 3, Matrix metalloproteinase-13, MMP-13, MMP13

      Key facts

      Isotype

      IgG

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Carrier free

      Yes

      Clone number

      EPR21778

      Purification technique

      Affinity purification Protein A

      Specificity

      ab219620 showed specific staining in cortical bone, but that no staining was observed in the breast cancer tissue tested.

      Concentration
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      Storage

      Shipped at conditions

      Blue Ice

      Appropriate long-term storage conditions

      +4°C

      Storage information

      Do Not Freeze

      Notes

      ab237604 is the carrier-free version of Anti-MMP13 antibody [EPR21778] ab219620.

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free batch production

      For more information, read more on recombinant antibodies.

      Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

      This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

      Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

      This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

      Supplementary info

      Activity summary

      Matrix metallopeptidase 13 (MMP-13) also called collagenase 3 is an enzyme with an approximate molecular mass of 54 kDa. It plays a significant role in the breakdown of extracellular matrix components particularly collagen. MMP-13 is expressed in several tissues including cartilage skin and bone. Its expression sees an increase during tissue remodeling and in pathological conditions. MMP-13 also participates in processes like wound healing and embryonic development making it a focal point for understanding tissue dynamics.

      Biological function summary

      Matrix metallopeptidase 13 contributes extensively to the degradation of collagen type II a major component of cartilage. As a zinc-dependent endopeptidase MMP-13 is part of the MMP family which facilitates the remodeling of the extracellular matrix. MMP-13 is involved in the proteolytic cascade working in conjunction with other MMPs and elastase to mediate tissue repair and turnover. It does not form complexes but acts in concert with other enzymes to execute its physiological functions effectively.

      Pathways

      Matrix metallopeptidase 13 significance is most noted in the cartilage degradation pathway. It interacts with other MMPs such as MMP-1 and MMP-9 to efficiently break down extracellular matrix components. These interactions highlight its role in the regulation of matrix metalloproteinase activity within the connective tissue degradation pathway. These pathways are key during normal connective tissue remodeling and in pathological processes illustrating the essential roles of MMPs in maintaining tissue homeostasis.

      Associated diseases and disorders

      Matrix metallopeptidase 13 has strong associations with osteoarthritis and rheumatoid arthritis. It contributes to cartilage destruction intensifying the progression of these disorders due to its potent collagenolytic activity. Other proteins like MMP-9 and MMP-14 are also involved in these processes potentially amplifying tissue damage in affected joints. Understanding how MMP-13 and related proteins drive these diseases offers potential therapeutic targets for slowing disease progression and enhancing joint health.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      4 product images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [EPR21778] - BSA and Azide free (ab237604), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [EPR21778] - BSA and Azide free (ab237604)

        Immunohistochemical analysis of paraffin-embedded rat femur tissue labeling MMP13 with Anti-MMP13 antibody [EPR21778] ab219620 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on cortical bone of rat femur (PMID: 22549931) is observed. Counter stained with hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MMP13 antibody [EPR21778] ab219620).

        Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [EPR21778] - BSA and Azide free (ab237604), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [EPR21778] - BSA and Azide free (ab237604)

        Immunohistochemical analysis of paraffin-embedded mouse femur tissue labeling MMP13 with Anti-MMP13 antibody [EPR21778] ab219620 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on cortical bone of mouse femur (PMID: 22549931) is observed. Counter stained with hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MMP13 antibody [EPR21778] ab219620).

        Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [EPR21778] - BSA and Azide free (ab237604), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [EPR21778] - BSA and Azide free (ab237604)

        Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling MMP13 with Anti-MMP13 antibody [EPR21778] ab219620 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Counter stained with hematoxylin.

        Negative control: No staining on human skeletal muscle (PMID: 9056642) is observed.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MMP13 antibody [EPR21778] ab219620).

        Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [EPR21778] - BSA and Azide free (ab237604), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [EPR21778] - BSA and Azide free (ab237604)

        Immunohistochemical analysis of paraffin-embedded human tibia tissue labeling MMP13 with Anti-MMP13 antibody [EPR21778] ab219620 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on cortical bone of human tibia (PMID: 22549931) is observed. Counter stained with hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MMP13 antibody [EPR21778] ab219620).

        Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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      Product protocols

      For this product, it's our understanding that no specific protocols are required. You can:

      Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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