Rabbit Recombinant Monoclonal MMP13 antibody. Carrier free. Suitable for IHC-P, WB, IP, ICC/IF and reacts with Mouse, Transfected cell lysate - Mouse, Transfected cell line - Mouse samples.
Images
![Immunoprecipitation - Anti-MMP13 antibody [EPR25858-87] - BSA and Azide free (AB315268), expandable thumbnail](https://content.abcam.com/products/images/mmp13-antibody-epr25858-87-bsa-and-azide-free-ab315268--immunoprecipitation-img424722.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-MMP13 antibody [EPR25858-87] - BSA and Azide free (AB315268)")
![Western blot - Anti-MMP13 antibody [EPR25858-87] - BSA and Azide free (AB315268), expandable thumbnail](https://content.abcam.com/products/images/mmp13-antibody-epr25858-87-bsa-and-azide-free-ab315268--western-blot-img424640.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MMP13 antibody [EPR25858-87] - BSA and Azide free (AB315268)")
![Western blot - Anti-MMP13 antibody [EPR25858-87] - BSA and Azide free (AB315268), expandable thumbnail](https://content.abcam.com/products/images/mmp13-antibody-epr25858-87-bsa-and-azide-free-ab315268--western-blot-img424639.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MMP13 antibody [EPR25858-87] - BSA and Azide free (AB315268)")
![Immunocytochemistry/ Immunofluorescence - Anti-MMP13 antibody [EPR25858-87] - BSA and Azide free (AB315268), expandable thumbnail](https://content.abcam.com/products/images/mmp13-antibody-epr25858-87-bsa-and-azide-free-ab315268--immunocytochemistry-immunofluorescence-img424712.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-MMP13 antibody [EPR25858-87] - BSA and Azide free (AB315268)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [EPR25858-87] - BSA and Azide free (AB315268), expandable thumbnail](https://content.abcam.com/products/images/mmp13-antibody-epr25858-87-bsa-and-azide-free-ab315268--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img424688.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [EPR25858-87] - BSA and Azide free (AB315268)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | IP | ICC/IF | |
|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Tested | Tested | Tested |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended |
| Transfected cell line - Mouse | Not recommended | Not recommended | Not recommended | Tested |
| Transfected cell lysate - Mouse | Not recommended | Tested | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Transfected cell lysate - Mouse, Transfected cell line - Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Transfected cell lysate - Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Transfected cell line - Mouse | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Transfected cell lysate - Mouse, Transfected cell line - Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Transfected cell line - Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Transfected cell lysate - Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CCN2. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CCN2. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.
Alternative names
Collagenase 3, Matrix metalloproteinase-13, MMP-13, Mmp13
Recommended products
Rabbit Recombinant Monoclonal MMP13 antibody. Carrier free. Suitable for IHC-P, WB, IP, ICC/IF and reacts with Mouse, Transfected cell lysate - Mouse, Transfected cell line - Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR25858-87
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab315268 is the carrier-free version of Anti-MMP13 antibody [EPR25858-87] ab315267.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Matrix metallopeptidase 13 (MMP-13) also called collagenase 3 is an enzyme with an approximate molecular mass of 54 kDa. It plays a significant role in the breakdown of extracellular matrix components particularly collagen. MMP-13 is expressed in several tissues including cartilage skin and bone. Its expression sees an increase during tissue remodeling and in pathological conditions. MMP-13 also participates in processes like wound healing and embryonic development making it a focal point for understanding tissue dynamics.
- Biological function summary
Matrix metallopeptidase 13 contributes extensively to the degradation of collagen type II a major component of cartilage. As a zinc-dependent endopeptidase MMP-13 is part of the MMP family which facilitates the remodeling of the extracellular matrix. MMP-13 is involved in the proteolytic cascade working in conjunction with other MMPs and elastase to mediate tissue repair and turnover. It does not form complexes but acts in concert with other enzymes to execute its physiological functions effectively.
- Pathways
Matrix metallopeptidase 13 significance is most noted in the cartilage degradation pathway. It interacts with other MMPs such as MMP-1 and MMP-9 to efficiently break down extracellular matrix components. These interactions highlight its role in the regulation of matrix metalloproteinase activity within the connective tissue degradation pathway. These pathways are key during normal connective tissue remodeling and in pathological processes illustrating the essential roles of MMPs in maintaining tissue homeostasis.
- Associated diseases and disorders
Matrix metallopeptidase 13 has strong associations with osteoarthritis and rheumatoid arthritis. It contributes to cartilage destruction intensifying the progression of these disorders due to its potent collagenolytic activity. Other proteins like MMP-9 and MMP-14 are also involved in these processes potentially amplifying tissue damage in affected joints. Understanding how MMP-13 and related proteins drive these diseases offers potential therapeutic targets for slowing disease progression and enhancing joint health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunoprecipitation - Anti-MMP13 antibody [EPR25858-87] - BSA and Azide free (ab315268), expandable thumbnail](https://content.abcam.com/products/images/mmp13-antibody-epr25858-87-bsa-and-azide-free-ab315268--immunoprecipitation-img424722.jpg "Immunoprecipitation - Anti-MMP13 antibody [EPR25858-87] - BSA and Azide free (ab315268)")
Immunoprecipitation - Anti-MMP13 antibody [EPR25858-87] - BSA and Azide free (ab315268)
This data was developed using Anti-MMP13 antibody [EPR25858-87] ab315267, the same antibody clone in a different buffer formulation.
MMP13 was immunoprecipitated from 0.35 mg Mouse articular cartilage tissue lysate with Anti-MMP13 antibody [EPR25858-87] ab315267 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-MMP13 antibody [EPR25858-87] ab315267 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse articular cartilage tissue lysate
Lane 2: Anti-MMP13 antibody [EPR25858-87] ab315267 IP in Mouse articular cartilage tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-MMP13 antibody [EPR25858-87] ab315267 in mouse articular cartilage tissue lysateAll lanes: Immunoprecipitation - Anti-MMP13 antibody [EPR25858-87] (Anti-MMP13 antibody [EPR25858-87] ab315267) at 1/30 dilution
All lanes: Mouse articular cartilage tissue lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 180s
![Western blot - Anti-MMP13 antibody [EPR25858-87] - BSA and Azide free (ab315268), expandable thumbnail](https://content.abcam.com/products/images/mmp13-antibody-epr25858-87-bsa-and-azide-free-ab315268--western-blot-img424640.jpg "Western blot - Anti-MMP13 antibody [EPR25858-87] - BSA and Azide free (ab315268)")
Western blot - Anti-MMP13 antibody [EPR25858-87] - BSA and Azide free (ab315268)
MMP13 Western blot staining using rabbit Anti-MMP13 antibody
This data was developed using Anti-MMP13 antibody [EPR25858-87] ab315267, the same antibody clone in a different buffer formulation.
This antibody does not cross-react with mouse MMP8 and MMP10.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-MMP13 antibody [EPR25858-87] (Anti-MMP13 antibody [EPR25858-87] ab315267) at 1/1000 dilution
Lane 1: 293T cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 5 µg
Lane 2: 293T cells transfected with a mouse MMP13 expression vector containing a myc-His-tag®, whole cell lysate at 5 µg
Lane 3: 293T cells transfected with a mouse MMP8 expression vector containing a myc-His-tag®, whole cell lysate at 5 µg
Lane 4: 293T cells transfected with a mouse MMP10 expression vector containing a myc-His-tag®, whole cell lysate at 5 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 48-60 kDa, 36 kDa
Exposure time: 3s
![Western blot - Anti-MMP13 antibody [EPR25858-87] - BSA and Azide free (ab315268), expandable thumbnail](https://content.abcam.com/products/images/mmp13-antibody-epr25858-87-bsa-and-azide-free-ab315268--western-blot-img424639.jpg "Western blot - Anti-MMP13 antibody [EPR25858-87] - BSA and Azide free (ab315268)")
Western blot - Anti-MMP13 antibody [EPR25858-87] - BSA and Azide free (ab315268)
MMP13 Western blot staining using rabbit Anti-MMP13 antibody
This data was developed using Anti-MMP13 antibody [EPR25858-87] ab315267, the same antibody clone in a different buffer formulation.
Negative control: brain, skeletal muscle (PMID: 8609233).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-MMP13 antibody [EPR25858-87] (Anti-MMP13 antibody [EPR25858-87] ab315267) at 1/1000 dilution
Lane 1: Mouse articular cartilage tissue lysate at 20 µg
Lane 2: Mouse brain tissue lysate at 20 µg
Lane 3: Mouse skeletal muscle tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 60 kDa, 48 kDa, 36 kDa
Exposure time: 6s
![Immunocytochemistry/ Immunofluorescence - Anti-MMP13 antibody [EPR25858-87] - BSA and Azide free (ab315268), expandable thumbnail](https://content.abcam.com/products/images/mmp13-antibody-epr25858-87-bsa-and-azide-free-ab315268--immunocytochemistry-immunofluorescence-img424712.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MMP13 antibody [EPR25858-87] - BSA and Azide free (ab315268)")
Immunocytochemistry/ Immunofluorescence - Anti-MMP13 antibody [EPR25858-87] - BSA and Azide free (ab315268)
This data was developed using Anti-MMP13 antibody [EPR25858-87] ab315267, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling MMP13 with Anti-MMP13 antibody [EPR25858-87] ab315267 at 1/1000 (0.491 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing positive staining in 293T cells transfected with a mouse MMP13 expression vector containing a myc tag, and showing no staining in 293T cells transfected with a mouse MMP8/MMP10/empty expression vector containing a myc tag. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-Myc tag antibody [9E10] ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [EPR25858-87] - BSA and Azide free (ab315268), expandable thumbnail](https://content.abcam.com/products/images/mmp13-antibody-epr25858-87-bsa-and-azide-free-ab315268--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img424688.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [EPR25858-87] - BSA and Azide free (ab315268)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [EPR25858-87] - BSA and Azide free (ab315268)
This data was developed using Anti-MMP13 antibody [EPR25858-87] ab315267, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cartilage tissue labeling MMP13 with Anti-MMP13 antibody [EPR25858-87] ab315267 at 1/100 (4.91 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse cartilage (PMID: 24108368). The section was incubated with Anti-MMP13 antibody [EPR25858-87] ab315267 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [EPR25858-87] - BSA and Azide free (ab315268), expandable thumbnail](https://content.abcam.com/products/images/mmp13-antibody-epr25858-87-bsa-and-azide-free-ab315268--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img424689.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [EPR25858-87] - BSA and Azide free (ab315268)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [EPR25858-87] - BSA and Azide free (ab315268)
This data was developed using Anti-MMP13 antibody [EPR25858-87] ab315267, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling MMP13 with Anti-MMP13 antibody [EPR25858-87] ab315267 at 1/100 (4.91 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on mouse skeletal muscle. The section was incubated with Anti-MMP13 antibody [EPR25858-87] ab315267 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [EPR25858-87] - BSA and Azide free (ab315268), expandable thumbnail](https://content.abcam.com/products/images/mmp13-antibody-epr25858-87-bsa-and-azide-free-ab315268--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img424689.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [EPR25858-87] - BSA and Azide free (ab315268)")
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