Rabbit Polyclonal MMP14 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 33 publications. Immunogen corresponding to Synthetic Peptide within Human MMP14 aa 450-550.
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Publications
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- International journal of molecular sciences 24:2023Dupuytren's Disease Is Mediated by Insufficient TGF-β1 Release and Degradation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLisa Oezel,Marie Wohltmann,Nele Gondorf,Julia Wille,Irmak Güven,Joachim Windolf,Simon Thelen,Carina Jaekel,Vera GrotheerPubMed 37894778
- Cancer immunology research 11:810-8292023Breast Cancer Stem Cell-Derived Tumors Escape from γδ T-cell Immunosurveillance In Vivo by Modulating γδ T-cell Ligands.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatrin Raute,Juliane Strietz,Maria Alejandra Parigiani,Geoffroy Andrieux,Oliver S Thomas,Klaus M Kistner,Marina Zintchenko,Peter Aichele,Maike Hofmann,Houjiang Zhou,Wilfried Weber,Melanie Boerries,Mahima Swamy,Jochen Maurer,Susana MinguetPubMed 37139603
- The Journal of cell biology 222:2023Proteolytic regulation of a galectin-3/Lrp1 axis controls osteoclast-mediated bone resorption.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLingxin Zhu,Yi Tang,Xiao-Yan Li,Samuel A Kerk,Costas A Lyssiotis,Xiaoyue Sun,Zijun Wang,Jung-Sun Cho,Jun Ma,Stephen J WeissPubMed 36880731
- Neuro-oncology advances 4:vdac1472022Targeting extracellular matrix remodeling sensitizes glioblastoma to ionizing radiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVarsha Thakur,Vijay S Thakur,Brittany Aguila,Tatiana I Slepak,Man Wang,Wei Song,Mohini Konai,Shahriar Mobashery,Mayland Chang,Ayush B Rana,Dazhi Wang,Juliano Tiburcio de Freitas,Sakir Humayun Gultekin,Scott M Welford,Michael E Ivan,Barbara BedogniPubMed 36212741
- Developmental cell 57:480-495.e62022Matrix remodeling controls a nuclear lamin A/C-emerin network that directs Wnt-regulated stem cell fate.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi Tang,Lingxin Zhu,Jung-Sun Cho,Xiao-Yan Li,Stephen J WeissPubMed 35150612
- Scientific reports 11:127872021ELV-N32 and RvD6 isomer decrease pro-inflammatory cytokines, senescence programming, ACE2 and SARS-CoV-2-spike protein RBD binding in injured cornea.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThang L Pham,Jiucheng He,Azucena H Kakazu,Jorgelina Calandria,Khanh V Do,Robert Nshimiyimana,Ting F Lam,Nicos A Petasis,Haydee E P Bazan,Nicolas G BazanPubMed 34140611
- Journal of neuroscience research 99:1048-10632021Glial cell line-derived neurotrophic factor increases matrix metallopeptidase 9 and 14 expression in microglia and promotes microglia-mediated glioma progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYimin Huang,Baole Zhang,Hannah Haneke,Verena Haage,Malgorzata Lubas,Yang Yuan,Pengfei Xia,Edyta Motta,Cynthia Nanvuma,Omar Dzaye,Feng Hu,Helmut KettenmannPubMed 33404121
- Cell and tissue research 383:707-7222020Presence of the matrix metalloproteinases during the migration of the primordial germ cells in zebrafish gonadal ridge.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTalita Sarah Mazzoni,Irani Quagio-GrassiottoPubMed 32960354
- Toxins 12:2020Inhibitory Effects of a Reengineered Anthrax Toxin on Canine Oral Mucosal Melanomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdriana Tomoko Nishiya,Marcia Kazumi Nagamine,Ivone Izabel Mackowiak da Fonseca,Andrea Caringi Miraldo,Nayra Villar Scattone,José Luiz Guerra,José Guilherme Xavier,Mário Santos,Cristina Oliveira Massoco de Salles Gomes,Jerrold Michael Ward,Shihui Liu,Stephen Howard Leppla,Thomas Henrik Bugge,Maria Lucia Zaidan DagliPubMed 32121654
- EMBO molecular medicine 12:e101542020Inhibition of Sema4D/PlexinB1 signaling alleviates vascular dysfunction in diabetic retinopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie-Hong Wu et. al.PubMed 31943789
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human MMP14 aa 450-550. The exact immunogen used to generate this antibody is proprietary information. Database link P50281
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/100.00000 | Notes - |
Associated Products
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Target data
Function
Endopeptidase that degrades various components of the extracellular matrix such as collagen (PubMed:8015608). Essential for pericellular collagenolysis and modeling of skeletal and extraskeletal connective tissues during development (By similarity). Activates progelatinase A/MMP2, thereby acting as a positive regulator of cell growth and migration (PubMed:22065321, PubMed:8015608). Involved in the formation of the fibrovascular tissues in association with pro-MMP2 (PubMed:12714657, PubMed:22065321). May be involved in actin cytoskeleton reorganization by cleaving PTK7 (PubMed:20837484). Acts as a regulator of Notch signaling by mediating cleavage and inhibition of DLL1 (PubMed:21572390). Cleaves ADGRB1 to release vasculostatin-40 which inhibits angiogenesis (PubMed:22330140). Acts as a negative regulator of the GDF15-GFRAL aversive response by mediating cleavage and inactivation of GFRAL (PubMed:35177851).
Alternative names
Matrix metalloproteinase-14, MMP-14, MMP-X1, Membrane-type matrix metalloproteinase 1, Membrane-type-1 matrix metalloproteinase, MT-MMP 1, MTMMP1, MT1-MMP, MT1MMP, MMP14
Recommended products
Rabbit Polyclonal MMP14 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 33 publications. Immunogen corresponding to Synthetic Peptide within Human MMP14 aa 450-550.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human MMP14 aa 450-550. The exact immunogen used to generate this antibody is proprietary information. Database link P50281
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Matrix metalloproteinase 14 (MMP14) also known as MT1-MMP is a membrane-bound enzyme involved in the breakdown of the extracellular matrix. This protease has a molecular weight of approximately 66 kDa and consists of various structural domains including a catalytic domain and a hinge region. MMP14 is expressed in many tissues including the stromal and cancer cells where it facilitates cell migration and tissue remodeling.
- Biological function summary
MMP14 influences extracellular matrix degradation enabling cellular invasion and migration. It exists as a part of macromolecular complexes at the cell surface interacting with other metalloproteinases. This interaction assists in the activation of other MMPs such as pro-MMP2 which further amplify matrix remodeling activities. The hinge region of MMP14 lends flexibility allowing it to effectively engage with substrate proteins.
- Pathways
MMP14 significantly affects cell signaling and tissue remodeling pathways. One critical pathway involves the activation of pro-MMP2 which is dependent on MMP14's proteolytic activity. MMP14 also plays a role in the regulation of cell proliferation and apoptosis through its interaction with proteins such as TIMP-2. This relationship between MMP14 and other regulatory proteins emphasizes its functional integration within major biological networks.
- Associated diseases and disorders
High MMP14 levels correlate strongly with cancer progression especially in cancers such as breast cancer and melanoma. The ability of MMP14 to degrade the extracellular matrix facilitates tumor invasion and metastasis. Through its regulatory activities MMP14 connects with other proteins like VEGF influencing angiogenesis within tumor microenvironments. Additionally MMP14 involvement in arthritic conditions highlights its role in joint degradation processes worsening the disease through augmented cartilage breakdown.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-MMP14 antibody (ab53712)
All lanes: Western blot - Anti-MMP14 antibody (ab53712) at 1 µg/mL
Lane 1: Human testis tissue lysate - total protein (ab30257) at 10 µg
Lane 2: K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 65 kDa
Observed band size: 100 kDa, 66 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP14 antibody (ab53712)
ab53712, at a 1/50 dilution, minus immunising peptide, staining Human MMP14 in Breast Carcinoma, using Immunohistochemsitry, Paraffin embedded tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP14 antibody (ab53712)
ab53712, at a 1/50 dilution, plus immunising peptide, staining Human MMP14 in Breast Carcinoma, using Immunohistochemstry, Paraffin embedded tissue.
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