AB53712

Anti-MMP14 antibody

Name: Anti-MMP14 antibody (ab53712) | Abcam
Brand: Abcam Limited
SKU: AB53712
Price: 585 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(39 Publications)

Rabbit Polyclonal MMP14 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 39 publications. Immunogen corresponding to Synthetic Peptide within Human MMP14 aa 450-550.

View Alternative Names

Matrix metalloproteinase-14, MMP-14, MMP-X1, Membrane-type matrix metalloproteinase 1, Membrane-type-1 matrix metalloproteinase, MT-MMP 1, MTMMP1, MT1-MMP, MT1MMP, MMP14

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP14 antibody (AB53712)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP14 antibody (AB53712)

ab53712, at a 1/50 dilution, minus immunising peptide, staining Human MMP14 in Breast Carcinoma, using Immunohistochemsitry, Paraffin embedded tissue.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP14 antibody (AB53712)

ab53712, at a 1/50 dilution, plus immunising peptide, staining Human MMP14 in Breast Carcinoma, using Immunohistochemstry, Paraffin embedded tissue.

Unknown

Western blot - Anti-MMP14 antibody (AB53712)

All lanes:

Western blot - Anti-MMP14 antibody (ab53712) at 1 µg/mL

Lane 1:

Human testis tissue lysate - total protein (<a href='/en-us/products/unavailable/human-testis-tissue-lysate-total-protein-ab30257'>ab30257</a>) at 10 µg

Lane 2:

K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 65 kDa

Observed band size: 100 kDa,66 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human MMP14 aa 450-550. The exact immunogen used to generate this antibody is proprietary information.

P50281

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Matrix metalloproteinase 14 (MMP14) also known as MT1-MMP is a membrane-bound enzyme involved in the breakdown of the extracellular matrix. This protease has a molecular weight of approximately 66 kDa and consists of various structural domains including a catalytic domain and a hinge region. MMP14 is expressed in many tissues including the stromal and cancer cells where it facilitates cell migration and tissue remodeling.

Biological function summary

MMP14 influences extracellular matrix degradation enabling cellular invasion and migration. It exists as a part of macromolecular complexes at the cell surface interacting with other metalloproteinases. This interaction assists in the activation of other MMPs such as pro-MMP2 which further amplify matrix remodeling activities. The hinge region of MMP14 lends flexibility allowing it to effectively engage with substrate proteins.

Pathways

MMP14 significantly affects cell signaling and tissue remodeling pathways. One critical pathway involves the activation of pro-MMP2 which is dependent on MMP14's proteolytic activity. MMP14 also plays a role in the regulation of cell proliferation and apoptosis through its interaction with proteins such as TIMP-2. This relationship between MMP14 and other regulatory proteins emphasizes its functional integration within major biological networks.

High MMP14 levels correlate strongly with cancer progression especially in cancers such as breast cancer and melanoma. The ability of MMP14 to degrade the extracellular matrix facilitates tumor invasion and metastasis. Through its regulatory activities MMP14 connects with other proteins like VEGF influencing angiogenesis within tumor microenvironments. Additionally MMP14 involvement in arthritic conditions highlights its role in joint degradation processes worsening the disease through augmented cartilage breakdown.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Endopeptidase that degrades various components of the extracellular matrix such as collagen (PubMed : 8015608). Essential for pericellular collagenolysis and modeling of skeletal and extraskeletal connective tissues during development (By similarity). Activates progelatinase A/MMP2, thereby acting as a positive regulator of cell growth and migration (PubMed : 22065321, PubMed : 8015608). Involved in the formation of the fibrovascular tissues in association with pro-MMP2 (PubMed : 12714657, PubMed : 22065321). May be involved in actin cytoskeleton reorganization by cleaving PTK7 (PubMed : 20837484). Acts as a regulator of Notch signaling by mediating cleavage and inhibition of DLL1 (PubMed : 21572390). Cleaves ADGRB1 to release vasculostatin-40 which inhibits angiogenesis (PubMed : 22330140). Acts as a negative regulator of the GDF15-GFRAL aversive response by mediating cleavage and inactivation of GFRAL (PubMed : 35177851).

See full target information MMP14

style

left-column

Publications (39)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:21630 PubMed40594223

2025

MMP14 and DDR2 are potential molecular markers for metastatic triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Rabeah Al-Temaimi,Rasheed Ahmad,Fahd Al-Mulla

Discover oncology 16:875 PubMed40407957

2025

Molecular subtype characteristics and development of prognostic model based on inflammation-related gene in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xuelei Hu,Tengfei Jiang,Jinxiang Wang

Scientific reports 15:2152 PubMed39820824

2025

Comprehensive investigation of matrix metalloproteinases in skin cutaneous melanoma: diagnostic, prognostic, and therapeutic insights.

Applications

Unspecified application

Species

Unspecified reactive species

Lingxia Wu,Chenxiaoxiao Liu,Weicai Hu

Discover oncology 15:677 PubMed39560824

2024

Flavokawain A suppresses the malignant progression of neuroblastoma in vitro depending on inactivation of ERK/VEGF/MMPs signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Mengyao Dong,Gaiqin Li,Geng Geng,Ming Ming,Yongtao Xu

Frontiers in cellular and infection microbiology 14:1308362 PubMed38476167

2024

Mechanisms of mesothelial cell response to viral infections: HDAC1-3 inhibition blocks poly(I:C)-induced type I interferon response and modulates the mesenchymal/inflammatory phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Flavia Trionfetti,Claudia Montaldo,Ivan Caiello,Giulio Bontempi,Michela Terri,Marta Tiberi,Vanessa Marchant,Alessandro Domenici,Paolo Menè,Marco Cordani,Clemens Zwergel,Giusi Prencipe,Marta Ruiz-Ortega,Sergio Valente,Antonello Mai,Marco Tripodi,Raffaele Strippoli

International journal of molecular sciences 24: PubMed37894778

2023

Dupuytren's Disease Is Mediated by Insufficient TGF-β1 Release and Degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Lisa Oezel,Marie Wohltmann,Nele Gondorf,Julia Wille,Irmak Güven,Joachim Windolf,Simon Thelen,Carina Jaekel,Vera Grotheer

Cancer immunology research 11:810-829 PubMed37139603

2023

Breast Cancer Stem Cell-Derived Tumors Escape from γδ T-cell Immunosurveillance In Vivo by Modulating γδ T-cell Ligands.

Applications

Unspecified application

Species

Unspecified reactive species

Katrin Raute,Juliane Strietz,Maria Alejandra Parigiani,Geoffroy Andrieux,Oliver S Thomas,Klaus M Kistner,Marina Zintchenko,Peter Aichele,Maike Hofmann,Houjiang Zhou,Wilfried Weber,Melanie Boerries,Mahima Swamy,Jochen Maurer,Susana Minguet

The Journal of cell biology 222: PubMed36880731

2023

Proteolytic regulation of a galectin-3/Lrp1 axis controls osteoclast-mediated bone resorption.

Applications

Unspecified application

Species

Unspecified reactive species

Lingxin Zhu,Yi Tang,Xiao-Yan Li,Samuel A Kerk,Costas A Lyssiotis,Xiaoyue Sun,Zijun Wang,Jung-Sun Cho,Jun Ma,Stephen J Weiss

Neuro-oncology advances 4:vdac147 PubMed36212741

2022

Targeting extracellular matrix remodeling sensitizes glioblastoma to ionizing radiation.

Applications

Unspecified application

Species

Unspecified reactive species

Varsha Thakur,Vijay S Thakur,Brittany Aguila,Tatiana I Slepak,Man Wang,Wei Song,Mohini Konai,Shahriar Mobashery,Mayland Chang,Ayush B Rana,Dazhi Wang,Juliano Tiburcio de Freitas,Sakir Humayun Gultekin,Scott M Welford,Michael E Ivan,Barbara Bedogni

Developmental cell 57:480-495.e6 PubMed35150612

2022

Matrix remodeling controls a nuclear lamin A/C-emerin network that directs Wnt-regulated stem cell fate.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Tang,Lingxin Zhu,Jung-Sun Cho,Xiao-Yan Li,Stephen J Weiss

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MMP14 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP14 antibody (AB53712)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP14 antibody (AB53712)

Western blot - Anti-MMP14 antibody (AB53712)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (39)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise