Anti-MMP14 antibody - Hinge region

• WB

AbReview18509****

Western blot - Anti-MMP14 antibody - Hinge region (AB38971)

Blocked using 5% milk for 1 hour at 20°C.

All lanes:

Western blot - Anti-MMP14 antibody - Hinge region (ab38971) at 1/5000 dilution

Lane 1:

Whole cell lysate prepared from HT1080 cells at 5 µg

Lane 2:

Whole cell lysate prepared from human vascular smooth muscle cells at 5 µg

Secondary

All lanes:

HRP conjugated donkey anti-rabbit IgG at 1/2000 dilution

Predicted band size: 65 kDa

Observed band size: 20 kDa,63 kDa,65 kDa

true

Exposure time: 30s

This image is a courtesy of an anonymous Abreview

• WB

Unknown

Western blot - Anti-MMP14 antibody - Hinge region (AB38971)

All lanes:

Western blot - Anti-MMP14 antibody - Hinge region (ab38971)

Lane 1:

Cell lysates from human dermal fibroblasts <i>neonatal</i> (no treatment)

Lane 2:

Cell lysates from human dermal fibroblasts <i>neonatal</i> (treated with II1beta)

Predicted band size: 65 kDa

Observed band size: 66 kDa

false

• WB

AbReview10483****

Western blot - Anti-MMP14 antibody - Hinge region (AB38971)

All lanes:

Western blot - Anti-MMP14 antibody - Hinge region (ab38971) at 1/1000 dilution

All lanes:

HT1080 whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated donkey anti-rabbit

Predicted band size: 65 kDa

Observed band size: 64 kDa

true

Exposure time: 1min

This image is courtesy of an anonymous Abreview

• WB

CiteAb

Western blot - Anti-MMP14 antibody - Hinge region (AB38971)

MMP14 western blot using anti-MMP14 antibody - Hinge region ab38971. Publication image and figure legend from Havre, P. A., Dang, L. H., et al., 2013, BMC Cancer, PubMed 24180670.

ab38971 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab38971 please see the product overview.

Erk(1/2) activation is highest in the parental Karpas 299 cell line. Cells (8 x 106) were grown in serum free media for 48 hrs, centrifuged at low speed to remove cells and debris, then at 100,000 x g for 1 hr. Vesicles were suspended in PBS and assayed for protein. Equal amounts of protein (5 μg) were loaded in each well of a 7.5% SDS gel. Following transfer to nitrocellulose, blots were probed with anti-MT1-MMP antibody (top) or anti-phospho-p44/42 MAPK antibody (middle), stripped, and reprobed with anti-p44/42 MAPK antibody (next to bottom). The blot was also probed with anti-α5 integrin antibody (bottom). Data are representative of two independent experiments.

false