Rabbit Polyclonal MMP14 antibody. Suitable for WB and reacts with Human samples. Cited in 40 publications. Immunogen corresponding to Synthetic Peptide within Human MMP14.
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- PloS one 18:e02848752023A standardized method for plasma extracellular vesicle isolation and size distribution analysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJ Nathaniel Diehl,Amelia Ray,Lauren B Collins,Andrew Peterson,Kyle C Alexander,Jacob G Boutros,John S Ikonomidis,Adam W AkermanPubMed 37115777
- PloS one 17:e02624792022Phenotypic characterization of primary cardiac fibroblasts from patients with HFpEF.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuhua Zhang,An O Van Laer,Catalin F Baicu,Lily S Neff,Stanley Hoffman,Marc R Katz,Sanford M Zeigler,Michael R Zile,Amy D BradshawPubMed 35015787
- Communications biology 4:10912021TC10 regulates breast cancer invasion and metastasis by controlling membrane type-1 matrix metalloproteinase at invadopodia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaren Hülsemann,Colline Sanchez,Polina V Verkhusha,Vera Des Marais,Serena P H Mao,Sara K Donnelly,Jeffrey E Segall,Louis HodgsonPubMed 34531530
- Biomolecules 11:2020AG-9, an Elastin-Derived Peptide, Increases In Vitro Oral Tongue Carcinoma Cell Invasion, through an Increase in MMP-2 Secretion and MT1-MMP Expression, in a RPSA-Dependent Manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesClara Bretaudeau et. al.PubMed 33396696
- Methods in molecular biology (Clifton, N.J.) 2230:303-3232020Assessment of Osteocytes: Techniques for Studying Morphological and Molecular Changes Associated with Perilacunar/Canalicular Remodeling of the Bone Matrix.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNeha S Dole,Cristal S Yee,Charles A Schurman,Sarah L Dallas,Tamara AllistonPubMed 33197021
- Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 35:196-2102019YAP and TAZ Mediate Osteocyte Perilacunar/Canalicular Remodeling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristopher D Kegelman,Jennifer C Coulombe,Kelsey M Jordan,Daniel J Horan,Ling Qin,Alexander G Robling,Virginia L Ferguson,Teresita M Bellido,Joel D BoerckelPubMed 31610061
- Cells 8:2019Endoglin Trafficking/Exosomal Targeting in Liver Cells Depends on -Glycosylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSteffen Meurer et. al.PubMed 31466384
- Molecular therapy. Nucleic acids 16:229-2452019Bone Marrow Mesenchymal Stem Cell-Derived Exosomal MicroRNA-126-3p Inhibits Pancreatic Cancer Development by Targeting ADAM9.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDong-Mei Wu et. al.PubMed 30925451
- FASEB journal : official publication of the Federa 32:2878-28902018MMP14 is a novel target of PTH signaling in osteocytes that controls resorption by regulating soluble RANKL production.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJesus Delgado-Calle et. al.PubMed 29401593
- Cell reports 21:2585-25962017Osteocyte-Intrinsic TGF-β Signaling Regulates Bone Quality through Perilacunar/Canalicular Remodeling.Applications:IHC-PReactive species:MouseNeha S Dole,Courtney M Mazur,Claire Acevedo,Justin P Lopez,David A Monteiro,Tristan W Fowler,Bernd Gludovatz,Flynn Walsh,Jenna N Regan,Sara Messina,Daniel S Evans,Thomas F Lang,Bin Zhang,Robert O Ritchie,Khalid S Mohammad,Tamara AllistonPubMed 29186693
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human MMP14. The exact immunogen used to generate this antibody is proprietary information. Database link P50281
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/5000.00000 | Notes When used against the reduced protein this antibody identifies bands at 65 Kd and 63 Kd (the pro-form and active form) as well as activation/breakdown products. |
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Target data
Function
Endopeptidase that degrades various components of the extracellular matrix such as collagen (PubMed:8015608). Essential for pericellular collagenolysis and modeling of skeletal and extraskeletal connective tissues during development (By similarity). Activates progelatinase A/MMP2, thereby acting as a positive regulator of cell growth and migration (PubMed:22065321, PubMed:8015608). Involved in the formation of the fibrovascular tissues in association with pro-MMP2 (PubMed:12714657, PubMed:22065321). May be involved in actin cytoskeleton reorganization by cleaving PTK7 (PubMed:20837484). Acts as a regulator of Notch signaling by mediating cleavage and inhibition of DLL1 (PubMed:21572390). Cleaves ADGRB1 to release vasculostatin-40 which inhibits angiogenesis (PubMed:22330140). Acts as a negative regulator of the GDF15-GFRAL aversive response by mediating cleavage and inactivation of GFRAL (PubMed:35177851).
Alternative names
Matrix metalloproteinase-14, MMP-14, MMP-X1, Membrane-type matrix metalloproteinase 1, Membrane-type-1 matrix metalloproteinase, MT-MMP 1, MTMMP1, MT1-MMP, MT1MMP, MMP14
Recommended products
Rabbit Polyclonal MMP14 antibody. Suitable for WB and reacts with Human samples. Cited in 40 publications. Immunogen corresponding to Synthetic Peptide within Human MMP14.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human MMP14. The exact immunogen used to generate this antibody is proprietary information. Database link P50281
- Purification technique
- Affinity purification Immunogen
- Specificity
This antibody binds to MMP14, but does not cross react with the other MMP family members (MMP1, MMP2, MMP3, MMP9).
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Matrix metalloproteinase 14 (MMP14) also known as MT1-MMP is a membrane-bound enzyme involved in the breakdown of the extracellular matrix. This protease has a molecular weight of approximately 66 kDa and consists of various structural domains including a catalytic domain and a hinge region. MMP14 is expressed in many tissues including the stromal and cancer cells where it facilitates cell migration and tissue remodeling.
- Biological function summary
MMP14 influences extracellular matrix degradation enabling cellular invasion and migration. It exists as a part of macromolecular complexes at the cell surface interacting with other metalloproteinases. This interaction assists in the activation of other MMPs such as pro-MMP2 which further amplify matrix remodeling activities. The hinge region of MMP14 lends flexibility allowing it to effectively engage with substrate proteins.
- Pathways
MMP14 significantly affects cell signaling and tissue remodeling pathways. One critical pathway involves the activation of pro-MMP2 which is dependent on MMP14's proteolytic activity. MMP14 also plays a role in the regulation of cell proliferation and apoptosis through its interaction with proteins such as TIMP-2. This relationship between MMP14 and other regulatory proteins emphasizes its functional integration within major biological networks.
- Associated diseases and disorders
High MMP14 levels correlate strongly with cancer progression especially in cancers such as breast cancer and melanoma. The ability of MMP14 to degrade the extracellular matrix facilitates tumor invasion and metastasis. Through its regulatory activities MMP14 connects with other proteins like VEGF influencing angiogenesis within tumor microenvironments. Additionally MMP14 involvement in arthritic conditions highlights its role in joint degradation processes worsening the disease through augmented cartilage breakdown.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-MMP14 antibody - Hinge region (ab38971)
All lanes: Western blot - Anti-MMP14 antibody - Hinge region (ab38971)
Lane 1: Cell lysates from human dermal fibroblasts neonatal (no treatment)
Lane 2: Cell lysates from human dermal fibroblasts neonatal (treated with II1beta)
Predicted band size: 65 kDa
Observed band size: 66 kDa
This image is courtesy of an anonymous customer reviewWestern blot - Anti-MMP14 antibody - Hinge region (ab38971)
All lanes: Western blot - Anti-MMP14 antibody - Hinge region (ab38971) at 1/1000 dilution
All lanes: HT1080 whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated donkey anti-rabbit
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 65 kDa
Observed band size: 64 kDa
Exposure time: 1min
This image is a courtesy of an anonymous customer reviewWestern blot - Anti-MMP14 antibody - Hinge region (ab38971)
Blocked using 5% milk for 1 hour at 20°C.
All lanes: Western blot - Anti-MMP14 antibody - Hinge region (ab38971) at 1/5000 dilution
Lane 1: Whole cell lysate prepared from HT1080 cells at 5 µg
Lane 2: Whole cell lysate prepared from human vascular smooth muscle cells at 5 µg
SecondaryAll lanes: HRP conjugated donkey anti-rabbit IgG at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 65 kDa
Observed band size: 20 kDa, 63 kDa, 65 kDa
Exposure time: 30s
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-MMP14 antibody - Hinge region (ab38971)
MMP14 western blot using anti-MMP14 antibody - Hinge region ab38971. Publication image and figure legend from Havre, P. A., Dang, L. H., et al., 2013, BMC Cancer, PubMed 24180670.
ab38971 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab38971 please see the product overview.
Erk(1/2) activation is highest in the parental Karpas 299 cell line. Cells (8 x 106) were grown in serum free media for 48 hrs, centrifuged at low speed to remove cells and debris, then at 100,000 x g for 1 hr. Vesicles were suspended in PBS and assayed for protein. Equal amounts of protein (5 μg) were loaded in each well of a 7.5% SDS gel. Following transfer to nitrocellulose, blots were probed with anti-MT1-MMP antibody (top) or anti-phospho-p44/42 MAPK antibody (middle), stripped, and reprobed with anti-p44/42 MAPK antibody (next to bottom). The blot was also probed with anti-α5 integrin antibody (bottom). Data are representative of two independent experiments.
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