Rabbit Polyclonal MMP16 antibody. Suitable for WB, IHC-P, IHC-Fr and reacts with Human, Rat samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human MMP16.
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Publications
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- International journal of biological sciences 19:2848-28592023Liver X receptors and estrogen receptor β, two players in a rare subtype of NSCLC.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWanfu Wu,Mozhgan Sarhadi,Xiaoyu Song,Jingling Xue,Yubing Dai,Jan-Ake GustafssonPubMed 37324952
- Bioengineered 12:12767-127772021Circular RNA hsa_circ_0002360 promotes non-small cell lung cancer progression through upregulating matrix metalloproteinase 16 and sponging multiple micorRNAs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYunting Zhang,Shaolin Zeng,Tao WangPubMed 34747300
- Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 54:e114592021CircRNA has_circ_0001806 promotes hepatocellular carcinoma progression via the miR-193a-5p/MMP16 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongmu Zhou,Ying ChenPubMed 34730679
- The Biochemical journal 478:3757-37742021PEPT1 is essential for the growth of pancreatic cancer cells: a viable drug target.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBradley K Schniers,Devaraja Rajasekaran,Ksenija Korac,Tyler Sniegowski,Vadivel Ganapathy,Yangzom D BhutiaPubMed 34569600
- Molecular cancer research : MCR 19:1441-14532021ATE1 Inhibits Liver Cancer Progression through RGS5-Mediated Suppression of Wnt/β-Catenin Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCong Xu,Yi-Ming Li,Bo Sun,Fang-Jing Zhong,Lian-Yue YangPubMed 34158395
- Cancer management and research 12:7087-70952020microRNA-26a Directly Targeting MMP14 and MMP16 Inhibits the Cancer Cell Proliferation, Migration and Invasion in Cutaneous Squamous Cell Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWang Zheng,Zong-Yu Li,De-Lai Zhao,Xing-Long Li,Rui LiuPubMed 32848463
- Translational cancer research 9:1795-18052020Exogenous HMGN2 inhibits the migration and invasion of osteosarcoma cell lines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEnjie Xu,Heng Jiang,Tao Lin,Yichen Meng,Xiao Ma,Jia Yin,Jun Ma,Xuhui ZhouPubMed 35117527
- BioMed research international 2019:56301242019Hypoxia-Inducible Factor 1A Upregulates HMGN5 by Increasing the Expression of GATA1 and Plays a Role in Osteosarcoma Metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEnjie Xu et. al.PubMed 31930127
- Artificial cells, nanomedicine, and biotechnology 47:3306-33142019Sevoflurane suppresses migration and invasion of glioma cells by regulating miR-146b-5p and MMP16.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLe Zhang,Jun Wang,Zhijie Fu,YanQiu Ai,Yanrong Li,Ying Wang,Yanping WangPubMed 31385537
- FEBS letters 593:2665-26742019Derepression of matrix metalloproteinase gene transcription and an emphysema-like phenotype in transcription factor Zbtb7c knockout mouse lungs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBu-Nam Jeon,Ji-Yang Song,Jin Won Huh,Woo-Ick Yang,Man-Wook HurPubMed 31222731
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human MMP16. The exact immunogen used to generate this antibody is proprietary information. Database link P51512
Reactivity data
Select an application| WB | IHC-P | IHC-Fr | |
|---|---|---|---|
| Human | Tested | Expected | Expected |
| Rat | Expected | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 0.50000-1.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 0.50000-1.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells.
Alternative names
C8orf57, MMPX2, MMP16, Matrix metalloproteinase-16, MMP-16, MMP-X2, Membrane-type matrix metalloproteinase 3, Membrane-type-3 matrix metalloproteinase, MT-MMP 3, MTMMP3, MT3-MMP, MT3MMP
Recommended products
Rabbit Polyclonal MMP16 antibody. Suitable for WB, IHC-P, IHC-Fr and reacts with Human, Rat samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human MMP16.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human MMP16. The exact immunogen used to generate this antibody is proprietary information. Database link P51512
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MMP16 also known as MT3-MMP (membrane-type 3 matrix metalloproteinase) is an enzyme part of the matrix metalloproteinase family. It plays a mechanical role in the degradation of extracellular matrix components. MMP16 has a molecular mass of 66 kDa and has proteolytic activity that contributes to tissue remodeling processes. This protein is a membrane-bound enzyme expressed in several tissues including the brain lungs and placenta. In the central nervous system it aids in maintaining extracellular environment by breaking down proteins.
- Biological function summary
MMP16 is essential in facilitating cellular activities such as migration invasion and angiogenesis. This protein operates as part of a complex that activates other MMPs including pro-MMP2 boosting their enzymatic activity. MMP16 participates in cellular remodeling by regulating the interactions between cells and their matrix which influences cell signaling and tissue morphogenesis.
- Pathways
MMP16 integrates into pathways relevant to extracellular matrix remodeling and cell signaling. It is actively involved in the Wnt and MAPK signaling pathways which influence various biological processes including development and cell proliferation. MMP16 also shows interactions with other MMP family members like MMP2 and MMP14 which collectively modulate the local microenvironment and cellular dynamics.
- Associated diseases and disorders
MMP16 is associated with cancer progression and neurodegenerative diseases. In cancer its upregulation correlates with tumor invasion and metastasis owing to its role in degradation of the basement membrane. MMP16 also contributes to neurodegenerative conditions like Alzheimer's Disease by affecting synaptic functions and neuron survival. It interacts with proteins like APP involved in Alzheimer's linking MMP16 to amyloid beta peptide processing and aggregation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-MMP16 antibody (ab73877)
All lanes: Western blot - Anti-MMP16 antibody (ab73877) at 1 µg/mL
Lane 1: Whole cell lysates prepared from Human Hela cells. at 50 µg
Lane 2: Whole cell lysates prepared from Human Jurkat cells. at 50 µg
Lane 3: Whole cell lysates prepared from Human Colo320 cells. at 50 µg
SecondaryAll lanes: HRP-conjugated Goat anti-rabbit IgG at 1/3000 dilution
Predicted band size: 70 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP16 antibody (ab73877)
Immunohistochemical analysis of paraffin-embedded sections
Rat kindey using MMP16 antibody ab73877 at 0.5 - 1 µg/ml.
Immunohistochemistry (Frozen sections) - Anti-MMP16 antibody (ab73877)
Immunohistochemical analysis of frozen sections
Rat kindey using MMP16 antibody ab73877 at 0.5 - 1 µg/ml.
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