JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB73877

Anti-MMP16 antibody

Be the first to review this product! Submit a review

|

(17 Publications)

Rabbit Polyclonal MMP16 antibody. Suitable for WB, IHC-P, IHC-Fr and reacts with Human, Rat samples. Cited in 17 publications. Immunogen corresponding to Synthetic Peptide within Human MMP16.

View Alternative Names

C8orf57, MMPX2, MMP16, Matrix metalloproteinase-16, MMP-16, MMP-X2, Membrane-type matrix metalloproteinase 3, Membrane-type-3 matrix metalloproteinase, MT-MMP 3, MTMMP3, MT3-MMP, MT3MMP

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP16 antibody (AB73877)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP16 antibody (AB73877)

Immunohistochemical analysis of paraffin-embedded sections Rat kindey using MMP16 antibody ab73877 at 0.5 - 1 µg/ml.

Immunohistochemistry (Frozen sections) - Anti-MMP16 antibody (AB73877)
  • IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-MMP16 antibody (AB73877)

Immunohistochemical analysis of frozen sections Rat kindey using MMP16 antibody ab73877 at 0.5 - 1 µg/ml.

Western blot - Anti-MMP16 antibody (AB73877)
  • WB

Unknown

Western blot - Anti-MMP16 antibody (AB73877)

All lanes:

Western blot - Anti-MMP16 antibody (ab73877) at 1 µg/mL

Lane 1:

Whole cell lysates prepared from Human Hela cells. at 50 µg

Lane 2:

Whole cell lysates prepared from Human Jurkat cells. at 50 µg

Lane 3:

Whole cell lysates prepared from Human Colo320 cells. at 50 µg

Secondary

All lanes:

HRP-conjugated Goat anti-rabbit IgG at 1/3000 dilution

Predicted band size: 70 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

IHC-Fr, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human MMP16. The exact immunogen used to generate this antibody is proprietary information.

P51512

style
left-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB92536

Anti-MMP2 antibody [EPR1184]

RabMAb|Recombinant

Western blot - Anti-MMP2 antibody [EPR1184] (AB92536)

  • 4
  • 6
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB92547

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB92547)

  • 5
  • 92
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "0.5-1 µg/mL", "IHCFr-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate) Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MMP16 also known as MT3-MMP (membrane-type 3 matrix metalloproteinase) is an enzyme part of the matrix metalloproteinase family. It plays a mechanical role in the degradation of extracellular matrix components. MMP16 has a molecular mass of 66 kDa and has proteolytic activity that contributes to tissue remodeling processes. This protein is a membrane-bound enzyme expressed in several tissues including the brain lungs and placenta. In the central nervous system it aids in maintaining extracellular environment by breaking down proteins.
Biological function summary

MMP16 is essential in facilitating cellular activities such as migration invasion and angiogenesis. This protein operates as part of a complex that activates other MMPs including pro-MMP2 boosting their enzymatic activity. MMP16 participates in cellular remodeling by regulating the interactions between cells and their matrix which influences cell signaling and tissue morphogenesis.

Pathways

MMP16 integrates into pathways relevant to extracellular matrix remodeling and cell signaling. It is actively involved in the Wnt and MAPK signaling pathways which influence various biological processes including development and cell proliferation. MMP16 also shows interactions with other MMP family members like MMP2 and MMP14 which collectively modulate the local microenvironment and cellular dynamics.

MMP16 is associated with cancer progression and neurodegenerative diseases. In cancer its upregulation correlates with tumor invasion and metastasis owing to its role in degradation of the basement membrane. MMP16 also contributes to neurodegenerative conditions like Alzheimer's Disease by affecting synaptic functions and neuron survival. It interacts with proteins like APP involved in Alzheimer's linking MMP16 to amyloid beta peptide processing and aggregation.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells.
See full target information MMP16
style
left-column

Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 14:1471267 PubMed39493455

2024

Identification and validation of matrix metalloproteinase hub genes as potential biomarkers for Skin Cutaneous Melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongyi Zhang,Mei Zhao,Zubing Zhou,Xiaodan Ren,Yunliang He,Tao Shen,Hongping Zeng,Kai Li,Yong Zhang

International journal of biological sciences 19:2848-2859 PubMed37324952

2023

Liver X receptors and estrogen receptor β, two players in a rare subtype of NSCLC.

Applications

Unspecified application

Species

Unspecified reactive species

Wanfu Wu,Mozhgan Sarhadi,Xiaoyu Song,Jingling Xue,Yubing Dai,Jan-Ake Gustafsson

Bioengineered 12:12767-12777 PubMed34747300

2021

Circular RNA hsa_circ_0002360 promotes non-small cell lung cancer progression through upregulating matrix metalloproteinase 16 and sponging multiple micorRNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Yunting Zhang,Shaolin Zeng,Tao Wang

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 54:e11459 PubMed34730679

2021

CircRNA has_circ_0001806 promotes hepatocellular carcinoma progression via the miR-193a-5p/MMP16 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hongmu Zhou,Ying Chen

The Biochemical journal 478:3757-3774 PubMed34569600

2021

PEPT1 is essential for the growth of pancreatic cancer cells: a viable drug target.

Applications

Unspecified application

Species

Unspecified reactive species

Bradley K Schniers,Devaraja Rajasekaran,Ksenija Korac,Tyler Sniegowski,Vadivel Ganapathy,Yangzom D Bhutia

Molecular cancer research : MCR 19:1441-1453 PubMed34158395

2021

ATE1 Inhibits Liver Cancer Progression through RGS5-Mediated Suppression of Wnt/β-Catenin Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Cong Xu,Yi-Ming Li,Bo Sun,Fang-Jing Zhong,Lian-Yue Yang

Cancer management and research 12:7087-7095 PubMed32848463

2020

microRNA-26a Directly Targeting MMP14 and MMP16 Inhibits the Cancer Cell Proliferation, Migration and Invasion in Cutaneous Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wang Zheng,Zong-Yu Li,De-Lai Zhao,Xing-Long Li,Rui Liu

Translational cancer research 9:1795-1805 PubMed35117527

2020

Exogenous HMGN2 inhibits the migration and invasion of osteosarcoma cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Enjie Xu,Heng Jiang,Tao Lin,Yichen Meng,Xiao Ma,Jia Yin,Jun Ma,Xuhui Zhou

BioMed research international 2019:5630124 PubMed31930127

2019

Hypoxia-Inducible Factor 1A Upregulates HMGN5 by Increasing the Expression of GATA1 and Plays a Role in Osteosarcoma Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Enjie Xu,Zhe Ji,Heng Jiang,Tao Lin,Jun Ma,Xuhui Zhou

Artificial cells, nanomedicine, and biotechnology 47:3306-3314 PubMed31385537

2019

Sevoflurane suppresses migration and invasion of glioma cells by regulating miR-146b-5p and MMP16.

Applications

Unspecified application

Species

Unspecified reactive species

Le Zhang,Jun Wang,Zhijie Fu,YanQiu Ai,Yanrong Li,Ying Wang,Yanping Wang
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com